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From 2014.igem.org

Sequencing results came back viable for…. SynZIP1_C SynZIP4_A,B,C SynZIP5 (there is a deletion in flag tag, but the insert is there) SpyTag A

Received HBD 108-111 (Primers to take the flag tag out of SynZIP backbones)

Transformed verified plasmids into LSR10 cells and in PHL cells Plated LSR10 and PHL cells

Received pKD46- pBAD from Peter/Bom (it’s a plasmid) pBAD is the name of the promoter Entire plasmid is temperature sensitive and cells with the plasmid must be grown up at 30 degrees