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From 2014.igem.org

Received Synzips 1 and 5 Gibsoned Synzip #1 and Synzip #2 into the CsgA-48 linker backbone Transformed Synzip #1 into Mach I cells

• Synzip #5 ← → Synzip #6
• Synzip #1 ← → Synzip #1

Transformed borrowed plasmid from Peter (CsgA, 48 linker, SpyTag) into PHL and Mach I cells