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From 2014.igem.org

We began minipreps on the following: Our retransformations of pHBD 5 and 35 Our gibsons of pHBD 39, 40, 41, 42, and 44. We finished minipreps of all of them.

We also innoculated 5 ml of LB crb with 3 lof culture for pHBD 5, 39, 40, 41, 42, and 44. We placed one tube in each of the 25 C and 30 C shaker for over the weekend. We had read that it takes 12-24 hours for the chromoproteins to be expressed, so we wanted to see if the longer time period would allow us to view the colors.

Activity Log Ran a gel of the PCR products from yesterday F24 does not seem to be the same size as the other other bands, when it should be, due to the indiscernible difference in band size of the 3 vectors Ran a gel again with our PCRed F24s and the original F24 plasmid The bands are still not the same size Will send our F24 along with the original F24 to be sequenced Used HBD5 (fwd) and HBD7 (rev) Examined the cell growth from the biocompatibility assays