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From 2014.igem.org

We began minipreps of pHBD 1, 2, 43, and 44.

We then ran our PCRs of fragments of pHBD 26 and 28 from yesterday on a gel:

pHBD 18 and 35 were successfully PCRed, so those were gel extracted. We then Gibsonned those fragments to created pHBD 26. We then transformed and plated pHBD 26.

We finished the miniprep of pHBD 43 and sequenced pHBD 43 and 44 (both of which failed).

We made Kan, Cm, and Crb plates

Finally, we made cell stocks of pHBD 1 and 2.

Set up overnight cultures of pHBD 35 and 44 (for miniprep and cell stocks).