Wednesday 27th August

Lab Work

B - Construction of the fusion protein (color)

PCR clean-up of colonies containing pGEMTeasy+chromoprotein

by Sean

Three colonies were selected from the gel prepared earlier by Laetitia, namely #3,#4, and #6. The three gel samples underwent PCR clean-up.

Gel electrophoresis of colonies containing pGEMTeasy+chromoprotein

by Sean

Gel containing the said colonies underwent PCR clean-up. 1µl of each result was used for the electrophoresis. Nothing appeared under UV.

D - lemon scent

Plasmide extraction

By Mélanie

Extraction of pPS5 with the phenol protocol (as previously described)

PCR of Limonene synthase (BBa762100)

by Mélanie

5 tubes to do a gradient

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