Friday 11th July

Lab work

Fill in the WIKI (Notebook and Protocols)

by Juliette & Romain

A - The chassis coli Odor free

Preparation of competent cells E.coli

by Maher and Sean

Competent strains used:

• DH5α
• MG1655Z1

Sean did the first strain, Maher did the second one.

Protocol

Electrophoresis

by Fabio and Marie

1. Cm FNR-RBS-AmilCP term (1)
2. Cm FNR-RBS-AmilCP term (2)
3. B0015 (1)
4. B0015 (2)
5. PCola-GES 10^-2 (1)
6. PCola-GES 10^-2 (1)
7. PCola-GES 10^-2 (2)
8. PCola-GES 10^-2 (2)
9. PJBG-6409 10^-2 (1)
10. PJBG-6409 10^-2 (2)
11. PJBG-6409 10^-2 (2)

Protocol

Members present:

• Instructors and advisors: Alice, Solenne and Sylvie.
• Students: Arnaud, Fabio, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain and Sean.

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