Thursday 21st August

Lab work

B - Construction of the fusion protein (color)

Transformation of DH5α with chromoprotein

by Sean

protocol

D - Lemon Scent

Transformation of DH5α with CAD

by Sean

protocol

Electro elution of pPS1 digested by Pac1

by Huang vu and laetitia

We extracted the DNA from the agarose band saves yesterday

protocol of electro elution

Gel electrophoresis

1. ladder SM0403 5µl
2. BBa_K762100 10µl
3. BBa_K517003 10µl
4. p cola 10µl
5. pPSI 10µl

Extraction of pPSI

By Mélanie

From preculture of bacteria with pPSI we extract the plasmid

protocol

Extraction of pGMETeasy with LS GS and PS

By Mélanie

Following the cloning made by Laeticia in pGMETeasy, i extract the plasmid (protocol)

and I do some stock of each stain

Ligation of CAD in pMCS5

by Eugene

We have made an electrophoresis of purified CAD and pMCS5.

Dephosphorylation of pMCS5

30 min incubation in 37°c

15min of inactivation 65°c

Ligation

night at 16°c

protocol

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