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Team:Paris Saclay/Notebook/August/22
From 2014.igem.org
Contents |
Friday 22nd August
Lab work
D - Lemon Scent
pPSI digestion
| components | volumes |
|---|---|
| pPSI | 40μl |
| FastDigest green buffer 10X | 5μl |
| PacI | 2µl |
| H2O | 2µl |
pPSI dephosphorylation
After 2 hours, add 1.5µL of Alkaline Phosphatase (AP) and let it 1 hour.
Then, inactivation of AP at 65°C during 15 minutes.
We've made a electrophoresis to check the digestion.
(photo)
Ligation of LS/GS/PS in pPSI
For each synthase, put:
| components | volumes |
|---|---|
| synthase | 10μl |
| pPSI | 2μl |
| buffer | 2µl |
| H2O | 5µl |
| ligase | 1µl |
And let it in the freezer during 3 hours.
