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Team:Exeter
From 2014.igem.org
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Exeter iGEM 2014
Our project has two aims: To create an organism capable of effectively degrading environmental contaminants; and to create and improve synthetic tools for the iGEM database.
Our first goal is to create a bacterial strain capable of degrading environmental contaminants such as TNT and Nitroglycerin. We are investigating two enzymes that can potentially perform this function, and will study their characteristics both in vivo and in vitro.
Our second aim is to study some of the most commonly used regulators (promoters and ribosome binding sequences) found in the iGEM Registry of Standard Parts to assess how reliable they are, by combining them in different contexts. The reliability of such parts is essential when designing and optimizing biological systems.
In addition to this we are also testing the fluorescent protein iLOV, originally added to the Registry by the Glasgow 2011 Team. iLOV has the potential to become an alternative reporter to the commonly used GFP (and derivatives) in E. coli, but a lack of characterization has hindered its use in iGEM.
These aspects of our project will come together to create an effective, synthetic, modular system capable of effective bioremediation.
Exeter | ERASE
ERASE-The Game!
