Team:Paris Saclay/Notebook/August/19

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Contents

Tuesday 19th August

Lab work

D - Lemon Scent

Digestion of PCR results

by Sean, Hoang Vu, Eugene

components volumes
PCR purified LS 15 μl
FastDigest green buffer 10X 2 μl
PacI 1 µl
H2O 2 µl
components volumes
PCR purified GS 15 μl
FastDigest green buffer 10X 2 μl
PacI 1 µl
H2O 2 µl
components volumes
PCR purified PS 15 μl
FastDigest green buffer 10X 2 μl
PacI 1 µl
H2O 2 µl


components volumes
PCR purified pPS1 30 μl
FastDigest green buffer 10X 4 μl
PacI 2 µl
H2O 4 µl


Purification of PCR products of limonen synthase

We used the PCR products of limonen synthase prepared previously : August 18th

The five samples were pooled in one and then purified using this protocol : protocol


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