Team:Paris Saclay/Notebook/August/19
From 2014.igem.org
Contents |
Tuesday 19th August
Lab work
D - Lemon Scent
Digestion of PCR results
by Sean, Hoang Vu, Eugene
components | volumes |
---|---|
PCR purified LS | 15 μl |
FastDigest green buffer 10X | 2 μl |
PacI | 1 µl |
H2O | 2 µl |
components | volumes |
---|---|
PCR purified GS | 15 μl |
FastDigest green buffer 10X | 2 μl |
PacI | 1 µl |
H2O | 2 µl |
components | volumes |
---|---|
PCR purified PS | 15 μl |
FastDigest green buffer 10X | 2 μl |
PacI | 1 µl |
H2O | 2 µl |
components | volumes |
---|---|
PCR purified pPS1 | 30 μl |
FastDigest green buffer 10X | 4 μl |
PacI | 2 µl |
H2O | 4 µl |
Purification of PCR products of limonen synthase
We used the PCR products of limonen synthase prepared previously : August 18th
The five samples were pooled in one and then purified using this protocol : protocol