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Team:Paris Saclay/Notebook/August/18
From 2014.igem.org
Contents |
Monday 18th August
Lab work
D - Lemon Scent
PCR of BBa_K517003
By Hoang Vu and Eugène
We realized a PCR to amplify the β-pinene synthase gene.
| components | volumes |
|---|---|
| DNA of GS | 1 μl |
| green goTaq buffer 5X | 10 μl |
| GoTaq enzyme | 0.5 µl |
| H2O | 29.5 µl |
| MgCl2 | 4 µl |
| dNTP | 1µl |
| IPS68bis | 2 µl |
| IPS69bis | 2 µl |
PCR of BBa_K762100
by Sean
Five tubes were prepared. Protocol was modified to match the one used for BBa_K517003 and p cola (PCR conducted on 8th August).
Primers used: iPS66 and iPS67.
PCR of pCola
by Laetitia and Melanie
We realized a PCR to amplify the geraniol synthase gene.
Five tubes were prepared. Protocol was modified to match the one used for pCola (PCR conducted on 8th August).
Primers used : iPS91bis and iPS82
