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Team:Paris Saclay/Notebook/August/18
From 2014.igem.org
Contents |
Monday 18th August
Lab work
D - Lemon Scent
PCR of BBa_K517003
By Hoang Vu and Eugène
We realized a PCR to amplify the β-pinene synthase gene.
| components | volumes |
|---|---|
| DNA of GS | 1 μl |
| green goTaq buffer 5X | 10 μl |
| GoTag enzyme | 0.5 µl |
| H2O | 29.5 µl |
| MgCl2 | 4 µl |
| dNTP | 1µl |
| IPS68bis | 2 µl |
| IPS69bis | 2 µl |
PCR of BBa_K762100
by Sean
Five tubes were prepared. Protocol was modified to match the one used for BBa_K517003 and p cola (PCR conducted on 8th August).
Primers used: iPS66 and iPS67.
PCR of pCola
"by Laetitia and Melanie" Five tubes were prepared. Protocol was modified to match the one used for pCola (PCR conducted on 8th August).
Primers used : iPS91bis and iPS82
