Lab Book

On this page you can find out more about how our project progressed over time. Our data was originally written in a standard lab book. We have scanned in each page of the book. Each page is listed with along with the page number, the date the page covers, a short description of work carried out that day as well as who did it.

Page No.	Date:	Description:	Carried out by:
1	30/06/14	Transformation of iGEM standardised DNA into plasmids for culturing. RBSs and protocols listed.	Martyn Bennet, Beth Hickton
2	31/06/14	Transformation of iGEM DNA into plasmids for culturing, cont. List of promoters transformed./td>	Beth Hickton, Edward Muir
3	01/07/14	List of promoters transformed, cont. Transformations were plated.	Beth Hickton, Martyn Bennet
4	02/07/14	The previous day transformations were plated.	Beth Hickton
5	02/07/14	Transformation of iGEM re-suspended promoters.	Beth Hickton
6	02/07/14	Preparation of competent E. coli cells	Beth Hickton
7	03/07/14	Promoter transformation results.	Jessica Rollit, Martyn Bennet
8	03/07/14	E. coli growth rate.	Edward Muir, Beth Hickton
9	04/07/14	Transformation of promoters and gene constructs.	Edward Muir, Beth Hickton
10	07/07/14	Putting constructs into the iGEM vector and testing for transformation efficiency.	Martyn Bennet, Edward Muir, Beth Hickton
11	08/07/14	Repeat construct addition into iGEM vector and testing transformation efficiency.	Edward Muir, Beth Hickton, Martyn Bennet
12	08/07/14	Transforming constructs into Top10 and DH5.	Beth Hickton, Martyn Bennet, Edward Muir
13	09/07/14	Results of Construction of iGEM vector.	Jessica Rollit, Beth Hickton
14	10/07/14	A range of mini-preps	Jessica Rollit, Beth Hickton
15	11/07/14	Measuring the length of DNA.	Edward Muir
16	11/07/14	Measuring the length of DNA, cont.	Edward Muir
17	11/07/14	Mini-prepping picked cultures containing promoters.	Callum Bailey, Elize Hernandez
18	11/07/14	Parts sent for sequencing	Callum Bailey, Elize Hernandez
19	11/07/14	Linearizing DNA.	Edward Muir
20	11/07/14	Creating glycerol stocks.	Beth Hickton, Max Smart
21	14/07/14	GFP and RFP resuspension and transformation.	Edward Muir, Martyn Bennet, Beth Hickton
22	14/07/14	GFP and RFP resuspension and transformation, cont.	Beth Hickton, Edward Muir
23	16/07/14	Mini-prepping GFP and RFP.	Jessica Rollit, Edward Muir, Fran Penrose
24	16/07/14	GFP and RFP digestion/ligation to PSB1C3.	Beth Hickton, Edward Muir
25	16/07/14	Plasmid digestions.	Edward Muir, Callum Bailey, Benjamin Miller
26	16/07/14	RFP/GFP ligations.	Edward Muir
27	16/07/14	RFP/GFP ligations, cont.	Edward Muir
28	17/07/14	Transformation of GFP/RFP with terminator.	Edward Muir
29	18/07/14	Making LB agar for promoters experiment.	Katie Pearce
30	18/07/14	Promoter experiment – quantities.	Edward Muir
31	18/07/14	Promoter experiment – quantities, cont.	Edward Muir
32	18/07/14	Promoter experiment – quantities, cont. 2.	Edward Muir
33	21/07/14	Promoter experiment preliminary timetable.	Edward Muir
34	22/07/14	Electrophoresis of RFP and GFP (with terminators).	Edward Muir
35	22/07/14	Preparation of competent E. coli cells.	Beth Hickton, Peter Reader
36	23/07/14	GFP/RFP + terminator liquid broths mini-prepped.	Beth Hickton, Peter Reader, Jessica Rollit
37	23/07/14	Potassium Chromate preparation.	Peter Reader, Benjamin Miller, Beth Hickton
38	23/07/14	Transformation of other promoters.	Edward Muir
39	24/07/14	Moving GFP+T and RFP+T onto AMP backbones.	Edward Muir, Katie Pearce
40	24/07/14	Ligation of GFP+T and RFP+T onto AMP backbones.	Edward Muir, Beth Hickton
41	24/07/14	Potassium Dichromate colorimetry and spectrophotometry.	Beth Hickton, Edward Muir
42	28/07/14	Plating Anderson RBSs.	Beth Hickton, Edward Muir, Elize Hernandez
43	28/07/14	Mini-prep of reporters, promoters and RBSs.	Beth Hickton, Elize Hernandez, Edward Muir
44	29/07/14	COSHH Form created for Nitroglycerine experiments.	Beth Hickton, Peter Reader, Benjamin Miller
45	30/07/14	Cr(VI) / Ligand experimentation.	Peter Reader
46	31/07/14	Mini-prep of constructs.	Edward Muir, Elize Hernandez, Beth Hickton
47	31/07/14	Digestion and ligation of promoter/RBS combos.	Edward Muir, Callum Bailey
48	31/07/14	Oligo preparation.	Edward Muir, Callum Bailey
49	31/07/14	Ligation of promoter and RBs digestions.	Edward Muir, Callum Bailey, Benjamin Miller
50	01/08/14	Digestion and Ligation of constructs 004 and 007.	Edward Muir, Jessica Rollit, Beth Hickton
51	01/08/14	Digestion and Ligation of constructs 004 and 007, cont.	Edward Muir
52	05/08/14	Colonies picked and mini-prepped.	Callum Bailey
53	05/08/14	Colonies picked and mini-prepped, cont.	Callum Bailey
54	06/08/14	Testing promoter/RBS combination efficiency.	Callum Bailey, Edward Muir
55	06/08/14	Testing promoter/RBS combination efficiency, cont.	Callum Bailey, Edward Muir
56	07/08/14	Picking colonies from promoter/RBS combination transformations.	Edward Muir, Callum Bailey
57	07/08/14	T. Cam experiment.	Edward Muir, Callum Bailey
58	11/08/14	Promoter/RBS combinations again.	Edward Muir, Callum Bailey
59	11/08/14	Promoter/RBS combinations again, cont.	Edward Muir, Callum Bailey
60	11/08/14	Inoculation of enzymes for construct 001 and 003.	Edward Muir, Callum Bailey
61	12/08/14	Inoculation of enzymes for construct 001 and 003, cont.	Edward Muir, Callum Bailey
62	12/08/14	Transformation of a sample promoter/RBS combination.	Callum Bailey
63	12/08/14	T. Cam experiment for solvents and ether.	Edward Muir
64	13/08/14	Combination transformation results and construct testing.	Edward Muir
65	13/08/14	Putting oligos into GFP and RFP.	Edward Muir

These lab book photos are a record of how we produced our synthetic bacterium. All experiments after this point are recorded in their relevant lab reports.

Exeter | ERASE

Team:Exeter/LabBook

From 2014.igem.org

Lab Book