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Team:Exeter/invivoactivity
From 2014.igem.org
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Measuring in vivo degredation using Raman spectroscopy
Introduction
Aim
The aim of this experiment was to measure the degradation rate of trinitrotoluene (TNT) and nitroglycerin (NG) in vivo using Raman spectroscopy. The degradation rate of enzymes Nem-A and Xen-B when transcribed by an E. coli bacterium will be measured. In addition to the degradation rates, a value for the partition coefficient for TNT and NG between water and the cell membrane was determined.
TNT Degradation
During the NemA and XenB-catalysed degradation of TNT, a series of nitrite groups as well aromatic ring reduction leads to formation of amino-dimethyl-tetranitrobiphenyl. During this process a hydride-Meisenheimer complex metabolite is formed. This metabolite has a distinct dark-brown colour [Vorbeck et.al 1994]
References
Vorbeck, Claudia; Lenke, Hiltrud; Fischer, Peter; Hans-Joachim, Knackmuss (1994) Identification of a Hydride-MeisenheimerComplex as a Metabolite of 2,4,6-Trinitrotoluene by a Mycobacterium StrainNavigation
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