Project

IGEM teams use live organism systems to do specific issues, as mechanics build a machine. Working with life still a lot of ethical questions like life representation and our teams has chosen Bioart to explore this subject. To illustrate these questions, we chose an object "made-in-nature", a lemon, and we tried to make an equivalent "made-in-IGEM". This object has the same external properties of a lemon: smell, form and a slowly changing colour from green to yellow, like a lemon when it rips.

In a collaboration with iGEM Paris-Bettencourt team in our iGEM French Meeting, we made a video to introduce the project.

Countdown

This page is under Marie's responsibility

• Deadline: 08/oct.
  • General introduction of the project.

• Deadline: 12/oct
  • Final review par Sylvie.

Inspirations

Scientific part

Our project is based on the construction of a bacterial lemon that will look like lemon but is made only of Escherichia coli modified to produce a lemon scent and to change colour from green to yellow. We will achieve our project by genetically modifying Escherichia coli to be devoid of any unpleasant odour by deleting the genes involved in E. coli's odor, by introducing genes for the production of the lemon scent and finally by adding a yellow-blue chromoprotein whose expression is controlled by a tRNA suppressor for the colour switching.

Remove the bad smell of E. coli

Our lemon should smell like a lemon. Our chassis is Escherichia coli , a bacterium known to have a foul odor. We thus have to remove the genes involved in this particular phenotype. The tnaA gene is required for the degradation of tryptophane into indole, the main molecule at the origin of E. coli smell. We use a phage transduction method to reach our goal.

Lemon Scent

The particular odor of lemon is mainly due to 3 monoterpenes: limonene, beta-pinene and geranial. We will clone the 3 monoterpene synthases responsible for the production of these 3 molecules into our bacteria to allow them to produce this fragrance. We will also improve the production of the GPP precursor using a synthetic mevalonate pathway using the construction of the team of T. S. Lee.

Lemon appearance and Ripening

Since vision is one of the most important senses in humans, we want the lemon look like as real as possible. To achieve this, we plan to make our lemon look green or yellow, as a real one. Furthermore, we also want to simulate the ripening process of the lemon by changing its color gradually from green to yellow.

Artistic Part

Lemon Shaping

We basically wanted to build up a lemon tree in which one we would have put lemons we had made. As lemons pass from green to yellow, we would have simulated the season’s cycle.

Boston Installation

Countdown

This page is under Leïla's responsibility

• Deadline: 08/oct.
  • Text
• Deadline: 12/oct
  • Final review by Solenne.