Team:Heidelberg/AwesomeSheet/LIGHTrun

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– LIGHTrun

Awesome Sheet PA Gels Dnmt1 Methylation Assay Blots Waste SURPREMErun Strains Stocks Samples Purifications Plate Reader Marker LIGHTRun Enzymes Competent cells Induction Growth 3AL AGels AssmProd Assembly GoldenGate Parts Submission Parts PCR products PCRs Plasmids Plates Primer Res SL Trafos

ABCDEFGHIJKL
1
BarcodeDatumTubePrimerH2OErgebnisAnalyser
2
IDV/NameV/µLIDNameV/µlName
3
76DB2508-20O55-9pSB1A3-Smt3-2ZSortA-H6 (ccdB)2.001VF22.55.50ok
4
76DB2608-20O55-10pSB1A3-Smt3-2ZSortA-H6 (ccdB)2.001VF22.55.50mixture
5
76DB2708-21O55-9pSB1A3-Smt3-2ZSortA-H6 (ccdB)2.002VR2.55.50bad sequencing
6
76DB2808-21O110-2pSBX1K3-2Zv2Npu:(LambdaLys-H6)1.501VF22.56.00several deletions
7
76DB298/25/2014O125pSB1C3-Smt3-2ZSortA-H6 (ccdB)2.001VF22.55.50negative, contains GFP
8
76DB308/25/2014O125pSB1C3-Smt3-2ZSortA-H6 (ccdB)2.002VR2.55.50negative, contains GFP
9
76DB318/25/2014O125-2pSB1C3-Smt3-2ZSortA-H6 (ccdB)1.501VF22.56.00negative, contains GFP
10
76DB328/25/2014O125-2pSB1C3-Smt3-2ZSortA-H6 (ccdB)1.502VR2.56.00negative, contains GFP
11
76DB338/25/2014O125-3pSB1C3-Smt3-2ZSortA-H6 (ccdB)1.201VF22.56.30negative, contains GFP
12
76DB348/25/2014O125-3pSB1C3-Smt3-2ZSortA-H6 (ccdB)1.202VR2.56.30negative, contains GFP
13
76DB358/25/2014O78pSBX1K3-T7RBS_BBv22.001VF22.55.50seems ok; some bases badly called
14
76DB368/26/2014O128pSBX1K3-T7RBS-H6-sfGFP-H61.831VF22.55.67perfect read, mutation in vf2 binding site??, insertion bbetween rbs and atg
15
76DB378/26/2014O128pSBX1K3-T7RBS-H6-sfGFP-H61.832VR2.55.67perfect read, mutation in vf2 binding site??, insertion bbetween rbs and atg
16
76DB38O55-11pSB1A3-Smt3-2ZSortA-H6 (ccdB)10.921VF22.5-3.42single base insertion in Smt3/ccdb CPEC homology region
17
76DB39O55-12pSB1A3-Smt3-2ZSortA-H6 (ccdB)1.851VF22.55.65single base deletion in Smt3/ccdb CPEC homology region
18
76DB40O55-13pSB1A3-Smt3-2ZSortA-H6 (ccdB)1.541VF22.55.96ok
19
76DB41O55-14pSB1A3-Smt3-2ZSortA-H6 (ccdB)1.921VF22.55.58ok
20
76DB42O55-15pSB1A3-Smt3-2ZSortA-H6 (ccdB)1.631VF22.55.87ok
21
76DB43O55-16pSB1A3-Smt3-2ZSortA-H6 (ccdB)2.091VF22.55.41ok
22
76DB44O55-17pSB1A3-Smt3-2ZSortA-H6 (ccdB)1.271VF22.56.23single base deletion in backbone/Smt3 CPEC homology region
23
76DB45O55-18pSB1A3-Smt3-2ZSortA-H6 (ccdB)1.181VF22.56.32ok
24
76DB46O55-19pSB1A3-Smt3-2ZSortA-H6 (ccdB)1.861VF22.55.64ok
25
76DB47O55-20pSB1A3-Smt3-2ZSortA-H6 (ccdB)1.981VF22.55.52ok
26
76DB48O55-21pSB1A3-Smt3-2ZSortA-H6 (ccdB)1.881VF22.55.62ok
27
76DB49O55-22pSB1A3-Smt3-2ZSortA-H6 (ccdB)1.921VF22.55.58ok
28
76DB50O55-23pSB1A3-Smt3-2ZSortA-H6 (ccdB)1.781VF22.55.72ok
29
76DB51O125-4pSB1C3-Smt3-2ZSortA-H6 (ccdB)3.141VF22.54.36negative, contains GFP
30
76DB52O125-5pSB1C3-Smt3-2ZSortA-H6 (ccdB)3.761VF22.53.74negative, contains GFP
31
76DB53O125-6pSB1C3-Smt3-2ZSortA-H6 (ccdB)4.341VF22.53.16negative, contains GFP
32
76DB54O125-7pSB1C3-Smt3-2ZSortA-H6 (ccdB)4.621VF22.52.88negative, contains GFP
33
76DB55O125-8pSB1C3-Smt3-2ZSortA-H6 (ccdB)5.851VF22.51.65negative, contains GFP
34
76DB56O125-9pSB1C3-Smt3-2ZSortA-H6 (ccdB)2.721VF22.54.78negative, contains GFP
35
76DB57O125-4pSB1C3-Smt3-2ZSortA-H6 (ccdB)3.142VR2.54.36negative, contains GFP
36
76DB58O125-5pSB1C3-Smt3-2ZSortA-H6 (ccdB)3.762VR2.53.74negative, contains GFP
37
76DB59O125-6pSB1C3-Smt3-2ZSortA-H6 (ccdB)4.342VR2.53.16negative, contains GFP
38
76DB60O125-7pSB1C3-Smt3-2ZSortA-H6 (ccdB)4.622VR2.52.88negative, contains GFP
39
76DB61O125-8pSB1C3-Smt3-2ZSortA-H6 (ccdB)5.852VR2.51.65negative, contains GFP
40
76DB62O125-9pSB1C3-Smt3-2ZSortA-H6 (ccdB)2.722VR2.54.78negative, contains GFP
41
76DB738/28/2014O124pSBX1K3-2Zv2Npu:Xyla(H6)destrCext2.001VF22.55.50insertions
42
76DB748/28/2014O124-3pSBX1K3-2Zv2Npu:Xyla(H6)destrCext2.001VF22.55.50ok
43
76DB758/28/2014O131pSBX4C5-2Zv2Npu:LaLys+ccdb2.001VF22.55.50gfp...............................
44
76DB768/28/2014O131-2pSBX4C5-2Zv2Npu:LaLys+ccdb2.001VF22.55.50ccdb missing
45
76DB778/28/2014O110-3pSBX1K3-2Zv2Npu:(LambdaLys-H6)2.001VF22.55.50a deletion
46
76DB788/28/2014O124pSBX1K3-2Zv2Npu:Xyla(H6)destrCext2.002VR2.55.50insertions
47
76DB798/28/2014O124-3pSBX1K3-2Zv2Npu:Xyla(H6)destrCext2.002VR2.55.50ok
48
76DB808/28/2014O131pSBX4C5-2Zv2Npu:LaLys+ccdb2.002VR2.55.50gfp...............................
49
76DB818/28/2014O131-2pSBX4C5-2Zv2Npu:LaLys+ccdb2.002VR2.55.50ccdb missing
50
76DB828/28/2014O110-3pSBX1K3-2Zv2Npu:(LambdaLys-H6)2.002VR2.55.50a deletion
51
76DB838/28/2014O132pSB1C3-Plac-GFP-IntN-MTS2.321VF22.55.18is actually psbx ssp h6 gfp
52
76DB848/28/2014O132pSB1C3-Plac-GFP-IntN-MTS2.322VR2.55.18is actually psbx ssp h6 gfp
53
76DB898/28/2014O129pSBX1K3-sfGFPN-NpuDnaEN_NpuDnaEC-sfGFPC#DIV/0!1VF22.5#DIV/0!ok
54
76DB908/28/2014O129pSBX1K3-sfGFPN-NpuDnaEN_NpuDnaEC-sfGFPC#DIV/0!2VR2.5#DIV/0!ok
55
#N/A#N/A#N/A2.5#N/A
56
#N/A#N/A#N/A2.5#N/A
57
#N/A#N/A#REF!#REF!2.5#N/A
58
76DB86#N/A#N/A#REF!#REF!2.5#N/A
59
76DB87#N/A#N/A#REF!#REF!2.5#N/A
60
76DB88#N/A#N/A#REF!#REF!2.5#N/A
61
76BD938/29/2014O111-3pSBX1K3-2Zv2Npu:(LambdaLys-Rigid)4.001VF22.53.50ok
62
76BD948/29/2014O111-3pSBX1K3-2Zv2Npu:(LambdaLys-Rigid)4.002VR2.53.50ok
63
76BD958/29/2014O111-4pSBX1K3-2Zv2Npu:(LambdaLys-Rigid)4.501VF22.53.00ok
64
76BD968/29/2014O111-4pSBX1K3-2Zv2Npu:(LambdaLys-Rigid)4.502VR2.53.00ok
65
76BD978/29/2014O110-4pSBX1K3-2Zv2Npu:(LambdaLys-H6)5.001VF22.52.50ok
66
76BD988/29/2014O110-4pSBX1K3-2Zv2Npu:(LambdaLys-H6)5.002VR2.52.50ok
67
76DB63O55-9pSB1A3-Smt3-2ZSortA-H6 (ccdB)2.00#N/A2.55.50ok
68
76DB64O55-13pSB1A3-Smt3-2ZSortA-H6 (ccdB)1.54#N/A2.55.96ok
69
76DB65O55-14pSB1A3-Smt3-2ZSortA-H6 (ccdB)1.92#N/A2.55.58ok
70
76DB66O55-15pSB1A3-Smt3-2ZSortA-H6 (ccdB)1.63#N/A2.55.87bad sequencing
71
76DB67O55-16pSB1A3-Smt3-2ZSortA-H6 (ccdB)2.09#N/A2.55.41ok
72
76DB68O55-18pSB1A3-Smt3-2ZSortA-H6 (ccdB)1.18#N/A2.56.32ok (one T not called)
73
76DB69O55-19pSB1A3-Smt3-2ZSortA-H6 (ccdB)1.86#N/A2.55.64ok
74
76DB70O55-20pSB1A3-Smt3-2ZSortA-H6 (ccdB)1.98#N/A2.55.52ok
75
76DB71O55-21pSB1A3-Smt3-2ZSortA-H6 (ccdB)1.88#N/A2.55.62mixture
76
76DB72O55-22pSB1A3-Smt3-2ZSortA-H6 (ccdB)1.92#N/A2.55.58ok
77
76DB34O55-23pSB1A3-Smt3-2ZSortA-H6 (ccdB)1.78#N/A2.55.72ok
78
76DC229/1/2014O106pSBX1K3-T7RBS-H6-NpuDnaE^C SN->AG-sfGFP^C-H62.141VF22.55.36ok
79
76DB999/1/2014O140pSB1C3-T7RBS_+His_SspDnaBC_Ext1.721VF22.55.78it has a his tag (the two plates were mixed up) -> juhu! intein protease ready
80
76DC009/1/2014O140pSB1C3-T7RBS_+His_SspDnaBC_Ext1.722VR2.55.78
81
76DC019/1/2014O131-4pSBX4C5-2Zv2Npu:LaLys+ccdb1.601VF22.55.90ok
82
76DC029/1/2014O131-4pSBX4C5-2Zv2Npu:LaLys+ccdb1.602VR2.55.90ok
83
76DC039/1/2014O141-2pSBX1K3-2Zv2Npu:LaLys+ccdb1.601VF22.55.90ok
84
76DC049/1/2014O141-2pSBX1K3-2Zv2Npu:LaLys+ccdb1.602VR2.55.90ok
85
76DC059/1/2014O131-7pSBX4C5-2Zv2Npu:LaLys+ccdb1.601VF22.55.90ok
86
76DC069/1/2014O131-7pSBX4C5-2Zv2Npu:LaLys+ccdb1.602VR2.55.90ok
87
76DC079/1/2014O131-8pSBX4C5-2Zv2Npu:LaLys+ccdb1.601VF22.55.90ok
88
76DC089/1/2014O131-8pSBX4C5-2Zv2Npu:LaLys+ccdb1.602VR2.55.90ok
89
76DC099/1/2014O141-6pSBX1K3-2Zv2Npu:LaLys+ccdb1.601VF22.55.90mutation in ccdb (R->H)
90
76DC109/1/2014O141-6pSBX1K3-2Zv2Npu:LaLys+ccdb1.602VR2.55.90mutation in ccdb (R->H)
91
76DC119/1/2014O108-2pSBX1K3-T7RBS-H6-sfGFP^N-C->G NpuDnaE^N-H61.501VF22.56.00mixture with T7RBS T6delJan
92
76DC129/1/2014O108-2pSBX1K3-T7RBS-H6-sfGFP^N-C->G NpuDnaE^N-H61.502VR2.56.00mixture with T7RBS T6delJan
93
76DC139/1/2014O108-4pSBX1K3-T7RBS-H6-sfGFP^N-C->G NpuDnaE^N-H60.901VF22.56.60okJan
94
76DC149/1/2014O108-4pSBX1K3-T7RBS-H6-sfGFP^N-C->G NpuDnaE^N-H60.902VR2.56.60okJan
95
76DC159/1/2014O128-2pSBX1K3-T7RBS-H6-sfGFP-H63.382VR2.54.12deletion in first stop codon, VF2C7T
Jan (without his brain)
96
76DC169/1/2014O128-3pSBX1K3-T7RBS-H6-sfGFP-H62.272VR2.55.23ok, VF2C7TJan
97
76DC179/1/2014O137-4pSBX4C5-1Gate-NpuDnaE(N)-H60.641VF22.56.86okJan
98
76DC189/1/2014O137-4pSBX4C5-1Gate-NpuDnaE(N)-H60.642VR2.56.86okJan
99
76DC209/1/2014O138-4pSBX4C5-H6-1GateNpuDnaE(C)0.871VF22.56.63okJan
100
76DC219/1/2014O138-410.872VR2.56.63okJan
101
76DC239/1/2014O113-3pSBX1K3-2Zv2Npu:Xyla_circ_noHis5.001VF22.52.50ok
102
76DC249/1/2014O113-3pSBX1K3-2Zv2Npu:Xyla_circ_noHis5.002VR2.52.50ok
103
76DB879/1/2014O113-4pSBX1K3-2Zv2Npu:Xyla_circ_noHis5.001VF22.52.50ok
104
76DB889/1/2014O113-4pSBX1K3-2Zv2Npu:Xyla_circ_noHis5.002VR2.52.50ok
105
76DB859/4/2014O159-3pSB1C3-K808000_T7RBS_+His_SspDnaBC_Ext2.391VF22.55.11ok
106
76DB869/4/2014O159-3pSB1C3-K808000_T7RBS_+His_SspDnaBC_Ext2.392VR2.55.11ok, juhu, one part of intein protease ready, you should ring bell
107
76DB919/8/2014O154-3pSBX4C5-H6-NpuC-mCherryC-H60.841VF22.56.66ok
108
76DB929/8/2014O154-3pSBX4C5-H6-NpuC-mCherryC-H60.842VR2.56.66ok
109
28HD7309-24O244pSBX4C5-2Zv2Npu:LaLys+bad10.902VR2.56.60ok
110
28HD7409-24O245pSBX4C5-2Zv2Npu:LaLys+may11.172VR2.56.33ok
111
28HD7509-24O246pSBX4C5-2Zv2Npu:LaLys+sgt10.972VR2.56.53kacke
112
28HD7609-24O247pSBX4C5-2Zv2Npu:LaLys+sgt20.922VR2.56.58kacke
113
28HD7709-24O248pSBX4C5-2Zv2Npu:LaLys+sho10.722VR2.56.78ok
114
28HD7809-24O249pSBX4C5-2Zv2Npu:LaLys+sho21.072VR2.56.43ok
115
28HD799/25/2014O251pSBX1K3-T7RBS-Smt3-2ZSortA:(G4-mDNMT1(731-1602)-0917/1r)-H65.0028
DNMT1_P4_F_deg.seq.731-1602
2.52.5096bp deletion
116
28HD809/25/2014O251-2pSBX1K3-T7RBS-Smt3-2ZSortA:(G4-mDNMT1(731-1602)-0917/1r)-H65.0028
DNMT1_P4_F_deg.seq.731-1602
2.52.5096bp deletion
117
28HD819/25/2014O252pSBX1K3-T7RBS-Smt3-2ZSortA:(G4-mDNMT1(731-1602)-0915/6f-FLAG)-H65.0028
DNMT1_P4_F_deg.seq.731-1602
2.52.50mixture
118
28HD829/25/2014O252-2pSBX1K3-T7RBS-Smt3-2ZSortA:(G4-mDNMT1(731-1602)-0915/6f-FLAG)-H65.0028
DNMT1_P4_F_deg.seq.731-1602
2.52.50probably ok, but short read; possibly G->V mutation in linker
119
28HD839/25/2014O253pSBX1K3-T7RBS-Smt3-2ZSortA:(G4-mDNMT1(731-1602)-0915/4r-FLAG)-H65.0028
DNMT1_P4_F_deg.seq.731-1602
2.52.50oktrafo'd
120
28HD849/25/2014O253-2pSBX1K3-T7RBS-Smt3-2ZSortA:(G4-mDNMT1(731-1602)-0915/4r-FLAG)-H65.0028
DNMT1_P4_F_deg.seq.731-1602
2.52.5063bp deletion
121
28HD859/25/2014O236pSBX1K3-T7RBS-H6-Smt3-0ZSortA5.0028
DNMT1_P4_F_deg.seq.731-1602
2.52.50wrong primer
122
28HD869/25/2014O236-2pSBX1K3-T7RBS-H6-Smt3-0ZSortA5.0028
DNMT1_P4_F_deg.seq.731-1602
2.52.50wrong primer
123
28HD879/25/2014O238pSBX1K3-T7RBS-H6-0ZSortA5.0028
DNMT1_P4_F_deg.seq.731-1602
2.52.50wrong primer
124
28HD889/25/2014O238-2pSBX1K3-T7RBS-H6-0ZSortA5.0028
DNMT1_P4_F_deg.seq.731-1602
2.52.50wrong primer
125
28HD899/25/2014O125-11pSB1C3-Smt3-2ZSortA-H6 (ccdB)5.001VF22.52.50ok
126
28HD909/25/2014O125-11pSB1C3-Smt3-2ZSortA-H6 (ccdB)5.002VR2.52.50ok
127
28HD919/25/2014O265pSBX1K3-T7RBS-Smt3-2ZSortA:(G2-mDNMT1(731-1602)-0917/1r)-H67.5028
DNMT1_P4_F_deg.seq.731-1602
2.50.00single base deletion
128
28HD929/25/2014O265-2pSBX1K3-T7RBS-Smt3-2ZSortA:(G2-mDNMT1(731-1602)-0917/1r)-H67.5028
DNMT1_P4_F_deg.seq.731-1602
2.50.00okto trafo
129
28HD949/25/2014O250pSBX1K3-T7RBS-FLAG-2ZNpu:(CWE-mDNMT1(731-1602)-0917/2r-H6SGRGT)7.5028
DNMT1_P4_F_deg.seq.731-1602
2.50.00oktrafo'd
130
28HD959/25/2014O250-2pSBX1K3-T7RBS-FLAG-2ZNpu:(CWE-mDNMT1(731-1602)-0917/2r-H6SGRGT)7.5028
DNMT1_P4_F_deg.seq.731-1602
2.50.00single silent mutation
131
28HD969/25/2014O254pSBX1K3-T7RBS-FLAG-2ZNpu:(CWE-mDNMT1(731-1602)-0915/3f-H6SGRGT)7.5028
DNMT1_P4_F_deg.seq.731-1602
2.50.00sequence ok; signal intensity decreased after DNMT1(trafo'd)
132
28HD939/25/2014O254-2pSBX1K3-T7RBS-FLAG-2ZNpu:(CWE-mDNMT1(731-1602)-0915/3f-H6SGRGT)7.5028
DNMT1_P4_F_deg.seq.731-1602
2.50.00okto trafo
133
28HD979/26/2014O236pSBX1K3-T7RBS-H6-Smt3-0ZSortA5.001VF22.52.50ok; start of the read (backbone) looks a bit strange
134
28HD989/26/2014O236pSBX1K3-T7RBS-H6-Smt3-0ZSortA5.002VR2.52.50ok
135
28HD999/26/2014O238pSBX1K3-T7RBS-H6-0ZSortA5.001VF22.52.50ok
136
28HE009/26/2014O238pSBX1K3-T7RBS-H6-0ZSortA5.002VR2.52.50ok
137
28HE019/26/2014O239-3pSBX1K3-T7RBS-2ZSortA:eGFP-H67.501VF22.50.00ok
138
28HE029/26/2014O239-2pSBX1K3-T7RBS-2ZSortA:eGFP-H67.502VR2.50.00ok
139
28HE039/26/2014O262pSBX1K3-T7RBS-FLAG-2ZNpu:(CWE-mDNMT1(731-1602)-mRFPexp-H5SGRGT)3.0025
DNMT1_P1_R_deg.seq.731-1602
2.54.50ok
140
28HE049/26/2014O262pSBX1K3-T7RBS-FLAG-2ZNpu:(CWE-mDNMT1(731-1602)-mRFPexp-H5SGRGT)3.0028
DNMT1_P4_F_deg.seq.731-1602
2.54.50ok
141
28HE499/29/2014O284pSBX1K3-2Zv2Npu:LaLys+bad12.502VR2.55.00no insert
142
28HE509/29/2014O285pSBX1K3-2Zv2Npu:LaLys+may12.502VR2.55.00ok
143
28HE519/29/2014O286pSBX1K3-2Zv2Npu:LaLys+sgt12.502VR2.55.00no insert
144
28HE529/29/2014O287pSBX1K3-2Zv2Npu:LaLys+sgt22.502VR2.55.00ok
145
28HE539/29/2014O288pSBX1K3-2Zv2Npu:LaLys+sho12.502VR2.55.00ok
146
28HE549/29/2014O289pSBX1K3-2Zv2Npu:LaLys+sho22.502VR2.55.00ok
147
28HE559/29/2014O290pSBX1K3-2Zv2Npu:LaLys+flexlinker2.502VR2.55.00ok
148
28HE569/29/2014O291pSBX1K3-2Zv2Npu:LaLys+bad22.502VR2.55.00bad
149
28HE579/29/2014O292pSBX1K3-2Zv2Npu:LaLys+bad32.502VR2.55.00bad
150
28HE589/29/2014O293pSBX1K3-2Zv2Npu:LaLys+bad42.502VR2.55.00bad
151
28HE599/29/2014O294pSBX1K3-2Zv2Npu:LaLys+may22.502VR2.55.00no insert
152
28HE609/29/2014O295pSBX1K3-2Zv2Npu:LaLys+may32.502VR2.55.00half insert
153
28HE619/29/2014O296-2pSBX1K3-2Zv2Npu:LaLys+ord12.502VR2.55.00ok
154
28HE629/29/2014O297pSBX1K3-2Zv2Npu:LaLys+ord22.502VR2.55.00deletions
155
28HE639/29/2014O298pSBX1K3-2Zv2Npu:LaLys+ord32.502VR2.55.00ok
156
28HE649/29/2014O299pSBX1K3-2Zv2Npu:LaLys+ord42.502VR2.55.00half insert
157
28HE659/29/2014O300pSBX1K3-2Zv2Npu:LaLys+ord52.502VR2.55.00no insert
158
28HE669/29/2014O301pSBX1K3-2Zv2Npu:LaLys+ord62.502VR2.55.00no insert
159
28HE679/29/2014O302pSBX1K3-2Zv2Npu:LaLys+sgt32.502VR2.55.00no insert
160
28HE689/29/2014O303pSBX1K3-2Zv2Npu:LaLys+sgt42.502VR2.55.00no insert
161
28HE699/29/2014O304pSBX1K3-2Zv2Npu:LaLys+sho32.502VR2.55.00no insert
162
28HE709/29/2014O305pSBX1K3-2Zv2Npu:LaLys+rigid_RGT2.502VR2.55.00insertions
163
28HE499/29/2014#N/A#N/A#REF!#REF!2.5#N/A
164
28HE509/29/2014#N/A#N/A#REF!#REF!2.5#N/A
165
28HE519/29/2014#N/A#N/A#REF!#REF!2.5#N/A
166
28HE529/29/2014#N/A#N/A#REF!#REF!2.5#N/A
167
28HE539/29/2014#N/A#N/A#REF!#REF!2.5#N/A
168
28HE549/29/2014#N/A#N/A#REF!#REF!2.5#N/A
169
28HE559/29/2014#N/A#N/A#REF!#REF!2.5#N/A
170
28HE569/29/2014#N/A#N/A#REF!#REF!2.5#N/A
171
28HE579/29/2014#N/A#N/A#REF!#REF!2.5#N/A
172
28HE589/29/2014#N/A#N/A#REF!#REF!2.5#N/A
173
28HE599/29/2014#N/A#N/A#REF!#REF!2.5#N/A
174
28HE609/29/2014#N/A#N/A#REF!#REF!2.5#N/A
175
28HE619/29/2014#N/A#N/A#REF!#REF!2.5#N/A
176
28HE629/29/2014#N/A#N/A#REF!#REF!2.5#N/A
177
28HE639/29/2014#N/A#N/A#REF!#REF!2.5#N/A
178
28HE649/29/2014#N/A#N/A#REF!#REF!2.5#N/A
179
28HE659/29/2014#N/A#N/A#REF!#REF!2.5#N/A
180
28HE669/29/2014#N/A#N/A#REF!#REF!2.5#N/A
181
28HE679/29/2014#N/A#N/A#REF!#REF!2.5#N/A
182
28HE689/29/2014#N/A#N/A#REF!#REF!2.5#N/A
183
28HE699/29/2014#N/A#N/A#REF!#REF!2.5#N/A
184
28HE709/29/2014#N/A#N/A#REF!#REF!2.5#N/A
185
28HE719/29/2014O273pSBX1K3-H6-DnaXC*-mRFPc-H6#N/A#REF!#REF!2.5#N/Ais pSBX1K3-H6-DnaXC-mRFPc-H6, mutation H->Q ?, short readJakob
186
28HE729/29/2014O273-2pSBX1K3-H6-DnaXC*-mRFPc-H6#N/A#REF!#REF!2.5#N/Abad read, end of mRFP okay
187
28HE449/29/2014O272pSBX1K3-H6-DnaXC-mRFPc-H6#N/A#REF!#REF!2.5#N/Aactually pSBX1K3-H6-DnaXC*-mRFPc-H6, construct or sequencing changed?
188
28HE459/29/2014O272-2pSBX1K3-H6-DnaXC-mRFPc-H6#N/A#REF!#REF!2.5#N/Aactually pSBX1K3-H6-DnaXN-sfGFP-H6,do not use frameshift
189
28HE469/29/2014O275pSBX1K3-SspDnaXc*-sfGFP^C-H6#N/A#REF!#REF!2.5#N/Aturned out to be SspDnaXN-sfGFPN, one additional His
190
28HE479/29/2014O275-2pSBX1K3-SspDnaXc*-sfGFP^C-H6#N/A#REF!#REF!2.5#N/Aturned out to be SspDnaXN-sfGFPN, frame shift in front of His
191
28HE489/29/2014O316-6pSBX4C5-H6-1GateNpu*(C)_v2#N/A1VF22.5#N/AokJan
192
28HE059/30/2014O227-5pSBX4C5-0GateTerDnaE3-S11C_mRFP169-225#N/A#REF!#REF!2.5#N/Agood, ok
193
28HE069/30/2014O308pSBX4C5-0GateSspDnaX-S11C*_mRFP*169-225#N/A#REF!#REF!2.5#N/ANpuDnaEC sequence in front of mRFPc not use
194
28HE079/30/2014O309-2pSBX4C5-0GateSspDnaX-S11N*_mRFP*1-168#N/A#REF!#REF!2.5#N/Ano DnaXN*
195
28HE089/30/2014O310pSBX4C5-0GateSspGyrB-S11C*_mRFP*169-2254.09#REF!#REF!2.53.41ok good, but not 310, DnaXC* inside (O308)
196
28HE099/30/2014O311pSBX4C5-0GateSspGyrB-S11N*_mRFP*1-168#DIV/0!#REF!#REF!2.5#DIV/0!ok good, but not 312 inside, DnaXN* inside (O309-2)
197
28HE109/30/2014O312pSBX4C5-0GateTerDnaE3-S11C*_mRFP*169-2253.91#REF!#REF!2.53.59ok good, but GyrBC* (O310) inside
198
28HE119/30/2014O313pSBX4C5-0GateTerDnaE3-S11N*_mRFP*1-1683.46#REF!#REF!2.54.04ok good, but GyrBN* (O311) inside
199
28HE419/30/2014O132-4pSB1C3-Plac-GFP-IntN-MTS3.381VF22.54.12
200
28HE429/30/2014O132-4pSB1C3-Plac-GFP-IntN-MTS3.912VR2.53.59
201
28HE409/30/2014O132-5pSB1C3-Plac-GFP-IntN-MTS3.381VF22.54.12
202
28HE399/30/2014O132-5pSB1C3-Plac-GFP-IntN-MTS3.912VR2.53.59
203
28HE239/30/2014O263pSB1C3-EX-BsaI-A-NN-IasB-OmpA#DIV/0!1VF22.5#DIV/0!
204
28HE249/30/2014O263pSB1C3-EX-BsaI-A-NN-IasB-OmpA3.912VR2.53.59
205
28HE259/30/2014O263-2pSB1C3-EX-BsaI-A-NN-IasB-OmpA#DIV/0!1VF22.5#DIV/0!ok
206
28HE269/30/2014O263-2pSB1C3-EX-BsaI-A-NN-IasB-OmpA#DIV/0!2VR2.5#DIV/0!ok
207
28HE1210/1/2014O117-6pSB1C3-BBa_K741002#DIV/0!1VF22.5#DIV/0!
208
28HE1310/1/2014O117-6pSB1C3-BBa_K741002#DIV/0!2VR2.5#DIV/0!
209
28HE2710/1/2014O364pSBX4C5-1Gate-NpuDnaE(N)-H6-H6-1GateNpuDnaE(C)2.461VF22.55.04ok, but only mRFP sequenced
210
28HE2810/1/2014O364pSBX4C5-1Gate-NpuDnaE(N)-H6-H6-1GateNpuDnaE(C)#DIV/0!2VR2.5#DIV/0!ok, but only mRFP sequenced
211
28HE2910/1/2014O365pSBX4C5-1GateNpuDnaE(N)*-H6-1GateNpuDnaE(C)*-H62.161VF22.55.34ok, but only mRFP sequenced
212
28HE3010/1/2014O365pSBX4C5-1GateNpuDnaE(N)*-H6-1GateNpuDnaE(C)*-H62.162VR2.55.34ok, but only mRFP sequenced
213
28HE3110/1/2014O366pSBX4C5-1GateNpuDnaE(N)*-H6-H6-1GateNpu*(C)_v22.241VF22.55.26ok, but only mRFP sequenced
214
28HE3210/1/2014O366pSBX4C5-1GateNpuDnaE(N)*-H6-H6-1GateNpu*(C)_v22.242VR2.55.26ok, but only mRFP sequenced
215
28HE1410/2/2014O280-3pSBX1K3-LOV-12-DnaXC-sfGFPc0.892VR2.56.61ok, good
216
28HE1810/2/2014O280-3pSBX1K3-LOV-12-DnaXC-sfGFPc0.891VF22.56.61ok, good
217
28HE1510/2/2014O267-3pSBX1K3-H6-mRFPn-DnaXN-H61.202VR2.56.30ok, good
218
28HE1610/2/2014O268-3pSBX1K3-H6-mRFPn-DnaXN*-H60.872VR2.56.63ok, good
219
28HE1710/2/2014O273-3pSBX1K3-H6-DnaXC*-mRFPc-H6#DIV/0!1VF22.5#DIV/0!many mutations
220
28HE1910/2/2014O272pSBX1K3-H6-DnaXC-mRFPc-H6#DIV/0!1VF22.5#DIV/0!ok good, but actually O273
221
28HE2010/2/2014O275-3pSBX1K3-SspDnaXc*-sfGFP^C-H6#DIV/0!1VF22.5#DIV/0!no actually DnaXN-GFPn
222
28HE2110/2/2014O310pSBX4C5-0GateSspGyrB-S11C*_mRFP*169-2253.381VF22.54.12ok, good but is actually DnaXC*-mRFPc (O308)
223
28HE3510/2/2014O311pSBX4C5-0GateSspGyrB-S11N*_mRFP*1-1683.912VR2.53.59ok, good, but actually DnaXN*-mRFPn (O309)
224
28HE3610/2/2014O312pSBX4C5-0GateTerDnaE3-S11C*_mRFP*169-225#DIV/0!1VF22.5#DIV/0!ok , good, but actually GyrBC*-mRFPc (O310)
225
28HE3710/2/2014O313pSBX4C5-0GateTerDnaE3-S11N*_mRFP*1-168#DIV/0!2VR2.5#DIV/0!ok, good, but actually GyrBN*-mRFPn (O311)
226
28HE3310/2/2014O314pSBX4C5-0GateNpuDnaE_C*_mRFP*169-225#DIV/0!1VF22.5#DIV/0!not use, actually DnaXN- GFPn
227
28HE3410/2/2014O314-2pSBX4C5-0GateNpuDnaE_C*_mRFP*169-225#DIV/0!1VF22.5#DIV/0!not use, actually DnaXN- GFPn
228
28HE4310/2/2014O346-3pSB1C3-EX-BsaI-CBD#DIV/0!1VF22.5#DIV/0!no.....GG could not work
229
#N/A#N/A#REF!#REF!2.5#N/A
230
O346-4#N/A#N/A#REF!#REF!2.5#N/A
231
O346-5#N/A#N/A#REF!#REF!2.5#N/A
232
O346-6#N/A#N/A#REF!#REF!2.5#N/A
233
O346-7#N/A#N/A#REF!#REF!2.5#N/A
234
O346-8#N/A#N/A#REF!#REF!2.5#N/A
235
O346-9#N/A#N/A#REF!#REF!2.5#N/A
236
O346-10#N/A#N/A#REF!#REF!2.5#N/A
237
O346-11#N/A#N/A#REF!#REF!2.5#N/A
238
O346-12#N/A#N/A#REF!#REF!2.5#N/A
239
O346-13#N/A#N/A#REF!#REF!2.5#N/A
240
O346-14#N/A#N/A#REF!#REF!2.5#N/A
241
O346-15#N/A#N/A#REF!#REF!2.5#N/A
242
O346-16#N/A#N/A#REF!#REF!2.5#N/A
243
O346-17#N/A#N/A#REF!#REF!2.5#N/A
244
O346-18#N/A#N/A#REF!#REF!2.5#N/A
245
O346-19#N/A#N/A#REF!#REF!2.5#N/A
246
O346-20#N/A#N/A#REF!#REF!2.5#N/A
247
O346-21#N/A#N/A#REF!#REF!2.5#N/A
248
O346-22#N/A#N/A#REF!#REF!2.5#N/A
249
O346-23#N/A#N/A#REF!#REF!2.5#N/A
250
O346-24#N/A#N/A#REF!#REF!2.5#N/A
251
O346-25#N/A#N/A#REF!#REF!2.5#N/A
252
O346-26#N/A#N/A#REF!#REF!2.5#N/A
253
O346-27#N/A#N/A#REF!#REF!2.5#N/A
254
O346-28#N/A#N/A#REF!#REF!2.5#N/A
255
O346-29#N/A#N/A#REF!#REF!2.5#N/A
256
O346-30#N/A#N/A#REF!#REF!2.5#N/A
257
O346-31#N/A#N/A#REF!#REF!2.5#N/A
258
O346-32#N/A#N/A#REF!#REF!2.5#N/A
259
O346-33#N/A#N/A#REF!#REF!2.5#N/A
260
O346-34#N/A#N/A#REF!#REF!2.5#N/A
261
O346-35#N/A#N/A#REF!#REF!2.5#N/A
262
O346-36#N/A#N/A#REF!#REF!2.5#N/A
263
O346-37#N/A#N/A#REF!#REF!2.5#N/A
264
O346-38#N/A#N/A#REF!#REF!2.5#N/A
265
O346-39#N/A#N/A#REF!#REF!2.5#N/A
266
O346-40#N/A#N/A#REF!#REF!2.5#N/A
267
O346-41#N/A#N/A#REF!#REF!2.5#N/A
268
O346-42#N/A#N/A#REF!#REF!2.5#N/A
269
O346-43#N/A#N/A#REF!#REF!2.5#N/A
270
O346-44#N/A#N/A#REF!#REF!2.5#N/A
271
O346-45#N/A#N/A#REF!#REF!2.5#N/A
272
O346-46#N/A#N/A#REF!#REF!2.5#N/A
273
O346-47#N/A#N/A#REF!#REF!2.5#N/A
274
O346-48#N/A#N/A#REF!#REF!2.5#N/A
275
O346-49#N/A#N/A#REF!#REF!2.5#N/A
276
O346-50#N/A#N/A#REF!#REF!2.5#N/A
277
O346-51#N/A#N/A#REF!#REF!2.5#N/A
278
O346-52#N/A#N/A#REF!#REF!2.5#N/A
279
O346-53#N/A#N/A#REF!#REF!2.5#N/A
280
O346-54#N/A#N/A#REF!#REF!2.5#N/A
281
O346-55#N/A#N/A#REF!#REF!2.5#N/A
282
O346-56#N/A#N/A#REF!#REF!2.5#N/A
283
O346-57#N/A#N/A#REF!#REF!2.5#N/A
284
O346-58#N/A#N/A#REF!#REF!2.5#N/A
285
O346-59#N/A#N/A#REF!#REF!2.5#N/A
286
O346-60#N/A#N/A#REF!#REF!2.5#N/A
287
O346-61#N/A#N/A#REF!#REF!2.5#N/A
288
O346-62#N/A#N/A#REF!#REF!2.5#N/A
289
O346-63#N/A#N/A#REF!#REF!2.5#N/A
290
O346-64#N/A#N/A#REF!#REF!2.5#N/A
291
O346-65#N/A#N/A#REF!#REF!2.5#N/A
292
O346-66#N/A#N/A#REF!#REF!2.5#N/A
293
O346-67#N/A#N/A#REF!#REF!2.5#N/A
294
O346-68#N/A#N/A#REF!#REF!2.5#N/A
295
O346-69#N/A#N/A#REF!#REF!2.5#N/A
296
O346-70#N/A#N/A#REF!#REF!2.5#N/A
297
O346-71#N/A#N/A#REF!#REF!2.5#N/A
298
O346-72#N/A#N/A#REF!#REF!2.5#N/A
299
O346-73#N/A#N/A#REF!#REF!2.5#N/A
300
O346-74#N/A#N/A#REF!#REF!2.5#N/A
301
O346-75#N/A#N/A#REF!#REF!2.5#N/A
302
O346-76#N/A#N/A#REF!#REF!2.5#N/A
303
O346-77#N/A#N/A#REF!#REF!2.5#N/A
304
O346-78#N/A#N/A#REF!#REF!2.5#N/A
305
O346-79#N/A#N/A#REF!#REF!2.5#N/A
306
O346-80#N/A#N/A#REF!#REF!2.5#N/A
307
O346-81#N/A#N/A#REF!#REF!2.5#N/A
308
O346-82#N/A#N/A#REF!#REF!2.5#N/A
309
O346-83#N/A#N/A#REF!#REF!2.5#N/A
310
O346-84#N/A#N/A#REF!#REF!2.5#N/A
311
O346-85#N/A#N/A#REF!#REF!2.5#N/A
312
O346-86#N/A#N/A#REF!#REF!2.5#N/A
313
O346-87#N/A#N/A#REF!#REF!2.5#N/A
314
O346-88#N/A#N/A#REF!#REF!2.5#N/A
315
O346-89#N/A#N/A#REF!#REF!2.5#N/A
316
O346-90#N/A#N/A#REF!#REF!2.5#N/A
317
O346-91#N/A#N/A#REF!#REF!2.5#N/A
318
O346-92#N/A#N/A#REF!#REF!2.5#N/A
319
O346-93#N/A#N/A#REF!#REF!2.5#N/A
320
O346-94#N/A#N/A#REF!#REF!2.5#N/A
321
O346-95#N/A#N/A#REF!#REF!2.5#N/A
322
O346-96#N/A#N/A#REF!#REF!2.5#N/A
323
O346-97#N/A#N/A#REF!#REF!2.5#N/A
324
O346-98#N/A#N/A#REF!#REF!2.5#N/A
325
O346-99#N/A#N/A#REF!#REF!2.5#N/A
326
O346-100
#N/A#N/A#REF!#REF!2.5#N/A
327
O346-101
#N/A#N/A#REF!#REF!2.5#N/A
328
O346-102
#N/A#N/A#REF!#REF!2.5#N/A
329
O346-103
#N/A#N/A#REF!#REF!2.5#N/A
330
O346-104
#N/A#N/A#REF!#REF!2.5#N/A
331
O346-105
#N/A#N/A#REF!#REF!2.5#N/A

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