Team:Heidelberg/AwesomeSheet/Stocks

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– Stocks

ABCDEFGHIJKLMNOPRSTUVW
1
All glyceol stocks are in box 1 in the -50^°C
OrganismOrigin
Related Prep
Related Sequenzing
Related Sequenzing (origin)
Related Induction
2
IDPersonDateGrowth
Storage Buffer
IDSpeciesStrainPlasmidsPID1PID2PID3ResistancesCommentsIDResultIDResultResult1Result 2
3
1Jan06-051C1
25% glycerol in LB
1E. coliTOP10#N/A#N/A
4
2Jan06-052C1
25% glycerol in LB
2E. coliDH10beta2#N/A
5
3Jan06-053C1
25% glycerol in LB
3E. coliTurbos#N/A
6
4Jan06-055C1
25% glycerol in LB
5E. coliOne Shot#N/A
7
5Jan06-054C1
25% glycerol in LB
4E. coliMG1655#N/A
8
6Jan06-056C1
25% glycerol in LB
6E. coliBI21(DE3)#N/A
9
7
Silvan, Jakob and Max W.
06-0743C1
25% glycerol in LB
3E. coliTurbosD2014-3-17EO7pSB1C3-BBa_I150167CmBF8247was previously thought to be BBa_C0012#N/A#N/A
10
8
Silvan, Jakob and Max W.
06-0742C1
25% glycerol in LB
3E. coliTurbosD2014-3-4GO6pSB1C3-BBa_R00106CmBF8249ok#N/A#N/A
11
9
Silvan, Jakob and Max W.
06-0710C1
25% glycerol in LB
3E. coliTurbosO12-2O12pUC1912Amp
12
10
Silvan, Jakob and Max W.
06-0740C1
25% glycerol in LB
3E. coliTurbosD2014-2-2NO4pSB1C3-BBa_C00124CmBF8250was prevoiously thought to be BBa_I15016#N/A#N/A
13
11Jakob, Jan06-0837C2
25% glycerol in LB
3E. coliTurbos
D2014-4-19D
O1BBa_J61002-BBa_J231101AmpBF8244ok#N/A#N/A
14
12Jakob, Jan06-0838C2
25% glycerol in LB
3E. coliTurbosD2014-4-21BO2BBa_J61002-BBa_J231182AmpBF8245ok#N/A#N/A
15
13Jakob, Jan06-0839C2
25% glycerol in LB
3E. coliTurbos
D2014-4-17D
O3BBa_J61002-BBa_J231003AmpBF8246ok#N/A#N/A
16
14Jakob, Jan06-0841C1
25% glycerol in LB
3E. coliTurbosD2014-2-24BO5pSB1C3-BBa_E02405CmBF8248
insertion before prefix leading to additional EcoRI site
#N/A#N/A
17
15Jakob, Jan06-0844C1
25% glycerol in LB
3E. coliTurbos
D2014-3-17G
O8pSB1C3-BBa_I150178CmBF8251ok#N/A#N/A
18
16Jakob06-1210
25% glycerol in LB
5E. coliOne ShotO13pSB1C3-pKH6insert13Cm
BF8252, BF8253
ok, ok
19
17Jakob06-1211
25% glycerol in LB
5E. coliOne ShotO14pSB1C3-pKH7insert14Cm
BF8253, BF8254
ok, ok
20
18Jakob06-1212
25% glycerol in LB
5E. coliOne ShotO15pSB1C3-pKH18insert15Cm
BF8254, BF8255
negativ, araC, negativ, Shewanella violacea
21
19Silvan06-2152C1
25% glycerol in LB
O16pGBMCS-SortA16AmpII2247ok
22
20Silvan06-2153C1
25% glycerol in LB
O17pHYRSF117KanII2232ok
23
21Silvan06-2154C1
25% glycerol in LB
O18pHYRSF1-0218KanII2233ok
24
22Silvan06-2155C1
25% glycerol in LB
O19pJJDuet3019KanII2234
Primer hat an 2 Stellen gebunden, sieht aber ok aus
25
23Silvan06-2156C1
25% glycerol in LB
O20pSFBAD0920AmpII2235ok
26
24Maggie, Silvan06-2557C1
25% glycerol in LB
3E. coliTurbosO22-0O22pFastBac-mDNMT122Amp
27
25Maggie, Silvan06-2558C1
25% glycerol in LB
3E. coliTurbosO23-0O23pRSFDuet-1-mDNMT1(731-1602)23Kan
II2225, II2227, II2228, II2229
ok, ok, ok, ok
28
26Maggie, Silvan06-2559C1
25% glycerol in LB
3E. coliTurbosO24-0O24pIT7724AmpII2236ok
29
27Maggie, Silvan06-2560C1
25% glycerol in LB
3E. coliTurbosO25-0O25pIT2925AmpII2237ok
30
28Maggie, Silvan06-2561C1
25% glycerol in LB
3E. coliTurbosO26-0O26pIT2126AmpII2238ok
31
29Maggie, Silvan06-2562C1
25% glycerol in LB
3E. coliTurbosO27-0O27pCL2027KanII2239ok
32
30Maggie, Silvan06-2563C1
25% glycerol in LB
3E. coliTurbosO28-0O28pAU0728AmpII2240ok
33
31Maggie, Silvan06-2564C1
25% glycerol in LB
3E. coliTurbosO29-0O29pVS4129AmpII2241ok
34
32Maggie, Silvan06-2565C1
25% glycerol in LB
3E. coliTurbosO30-0O30pVS0730KanII2242ok
35
33Silvan07-0366C1
25% glycerol in LB
7E. coli
Rosetta (DE3)
O31pRARE31CmII2224ok, aber falsche Richtung
36
34Jan07-06153C1
25% glycerol in LB
1E. coliTOP10S38O38pSB1AK8-BBa_I712074-BBa_I1501638Amp, Kan#N/A#N/A
37
35Jan07-06154C1
25% glycerol in LB
1E. coliTOP10S39O39pSB1AK8-BBa_I712074-BBa_I1501739Amp, Kan#N/A#N/A
38
36Jan07-06155C1
25% glycerol in LB
1E. coliTOP10S40O40pSB1AK8-BBa_I712074-BBa_E024040Amp, Kan#N/A#N/A
39
37Jan07-06156C2
25% glycerol in LB
1E. coliTOP10S41O41pSB1AK8-BBa_I712074-S-BBa_J7039041Amp, Kan#N/A#N/A
40
38Jan07-06142C1
25% glycerol in LB
3E. coliTurbosD2013-5-7JO37pSB1A2-BBa_S0352037Amp#N/A#N/A
41
39Caro7/8/2014157C2
25% glycerol in LB
3E. coliTurbosA32#2O32-2pSB1C3-2ZNpuH632Cm
II2245, II2246
insert in the prefix, single insertion in the ccdb part single deletion in the N Intein part, insert in the prefix, single insertion in the ccdb part single deletion in the N Intein part
#N/A#N/A
42
40Silvan7/4/2014131C1
25% glycerol in LB
7E. coli
Rosetta (DE3)
O23O23-2pRSFDuet-1-mDNMT1(731-1602), pRARE2331KanCm
II2225, II2227, II2228, II2229
ok, ok, ok, ok
43
41TODO!
25% glycerol in LB
3E. coliTurbos55Amp
< insert growth id
44
42Caro7/8/2014158C1
25% glycerol in LB
3E. coliTurbosA33O33pSB1C3-2ZNpu33Cm
II2248, II2249
insertion in the prefix, additional His after Flag, bad sequencing
#N/A#N/A
45
42bCaro7/8/2014158C2
25% glycerol in LB
3E. coliTurbosA33O33-2pSB1C3-2ZNpu33CmdiscardII2252
additional His after flag, insertion in the prefix
#N/A#N/A
46
42cCaro7/8/2014158C3
25% glycerol in LB
3E. coliTurbosA33O33-3pSB1C3-2ZNpu33CmdiscardII2253
additional His after flag, insertion in the prefix
#N/A#N/A
47
43Caro7/8/2014134C1
25% glycerol in LB
7E. coli
Rosetta (DE3)
O30pVS07, pRARE3031Kan, Cm#N/AII2242ok
48
44Caro7/8/2014137C1
25% glycerol in LB
7E. coli
Rosetta (DE3)
O29pVS41, pRARE2931Amp, Cm#N/AII2241ok
49
45Caro7/8/2014162C1
25% glycerol in LB
7E. coli
Rosetta (DE3)
O29
O29,30,31-3
pVS41, pVS07, pRARE293031Amp, Kan, CmII2241ok
50
46Silvan07-08164C1
25% glycerol in LB
1E. coliTOP10D2014-3-12IO43pSB1C3-BBa_I74690743Cm
II2250, II2251
, #N/A#N/A
51
47Jan07-03129C3
25% glycerol in LB
3E. coliTurbosA35O35-2pSB1C3-2ZSsp35Cm
II2230, II2243
ok, ok#N/A#N/A
52
48Jan07-1166C4
25% glycerol in LB
7E. coli
Rosetta (DE3)
pRARE31Cm#N/A
53
49Jan07-06133C1
25% glycerol in LB
7E. coli
Rosetta (DE3)
O17pHYRSF1, pRARE1731Kan, Cm#N/AII2232ok
54
50Jan07-03130C1
25% glycerol in LB
3E. coliTurbosD2013-5-6NO36pSB1AK8-BBa_I71207436AmpT7 promII2226
RFC23 (missing the final G in the prefix and the primary T in the suffix)
#N/A#N/A
55
51Jan07-11170C1-1
25% glycerol in LB
7E. coli
Rosetta (DE3)
O39pSB1AK8-BBa_I712074-BBa_I15017, pRARE3931Amp, Kan, Cm#N/A
56
52Jan07-11171C1-1
25% glycerol in LB
7E. coli
Rosetta (DE3)
O40pSB1AK8-BBa_I712074-BBa_E0240, pRARE4031Amp, Kan, Cm#N/A
57
53Jan07-11172C1-1
25% glycerol in LB
7E. coli
Rosetta (DE3)
O41pSB1AK8-BBa_I712074-S-BBa_J70390, pRARE4131Amp, Kan, Cm#N/A
58
54Silvan7/12/2014178C1
25% glycerol in LB
3E. coliTurbosS47#3O47pSB1AK8-T7-2ZNpu47Amp
II2263, II2265
, #N/A#N/A
59
55Silvan7/12/2014180C1
25% glycerol in LB
1E. coliTOP10O36pSB1AK8-BBa_I71207436Amp#N/AII2226
RFC23 (missing the final G in the prefix and the primary T in the suffix)
60
56Silvan7/21/2014211C2
25% glycerol in LB
3E. coliTurbosA55O55pSB1A3-Smt3-2ZSortA-H6 (ccdB)55Amp
II2267, II2268
, #N/A#N/A
61
57Jan7/24/2014209C2
25% glycerol in LB
5E. coliOne ShotA55O55-2pSB1A3-Smt3-2ZSortA-H6 (ccdB)55AmpII2269#N/A#N/A
62
58Jan7/24/2014211C1
25% glycerol in LB
3E. coliTurbosA55O55-3pSB1A3-Smt3-2ZSortA-H6 (ccdB)55AmpII2270#N/A#N/A
63
59Jan7/24/2014212C1
25% glycerol in LB
3E. coliTurbosA55#2O55-4pSB1A3-Smt3-2ZSortA-H6 (ccdB)55AmpII2271#N/A#N/A
64
60Jan7/24/2014221C1
25% glycerol in LB
1E. coliTOP10O53O53-2pSB1A2-BBa_I1350753Amp
65
61Jan7/24/2014218C1
25% glycerol in LB
1E. coliTOP10O50O50-2pSB4A5-BBa_J0445050Amp
66
62Jan7/24/2014217C1
25% glycerol in LB
1E. coliTOP10O49O49-3pSB1A3-BBa_J0445049Amp
67
63Jan7/24/2014219C1
25% glycerol in LB
1E. coliTOP10O51O51-2pSB4C5-BBa_J0445051Cm
68
64Jan7/24/2014220C1
25% glycerol in LB
1E. coliTOP10O52O52-2pSB4K5-BBa_J0445052Kan
69
65Jan7/24/2014222C1
25% glycerol in LB
1E. coliTOP10O54O54-2pSB1K3-BBa_J0445054Kan
70
66Jan7/24/2014227C1
25% glycerol in LB
3E. coliTurbosO64-2O64-4pSB1T3-BBa_J0445064Tet
71
67Jan7/24/2014224C1
25% glycerol in LB
3E. coliTurbosD2014-4-8DO66pSB3T5-BBa_J0445066Tet#N/A#N/A
72
68Jan07-26234C1
25% glycerol in LB
3E. coliTurbosL57#5O57-2pSBX1A3-BBa_I1350757Amp
FG1165, FG1198
sequenzing starts ok, then mixture?, ok#N/A#N/A
73
69Jan07-26235C1
25% glycerol in LB
3E. coliTurbosL58#3O58-3pSBX1C3-BBa_I1350758Cm#N/A#N/A
74
70Jan07-26236C1
25% glycerol in LB
3E. coliTurbosL59#5O59-3pSBX1K3-BBa_I1350759Kan
FG1167, FG1177, FG1199
frameshift, two fucking huge insertions, ok,longest,
#N/A#N/A
75
71Jan07-26238C1
25% glycerol in LB
3E. coliTurbosL61#3O61pSBX4A5-BBa_I1350761AmpFG1169starts ok then mixture#N/A#N/A
76
72Jan07-26239C1
25% glycerol in LB
3E. coliTurbosL62#3O62pSBX4C5-BBa_I1350762CmFG1170ok#N/A#N/A
77
73Jan07-26240C1
25% glycerol in LB
3E. coliTurbosL63#3O63pSBX4K5-BBa_I1350763KanFG1187bad sequencing#N/A#N/A
78
74Jan07-26234C2
25% glycerol in LB
3E. coliTurbosL57#5O57-3pSBX1A3-BBa_I1350757AmpFG1173bad sequencing#N/A#N/A
79
75Jan07-26235C2
25% glycerol in LB
3E. coliTurbosL58#3O58-4pSBX1C3-BBa_I1350758CmFG1175ok#N/A#N/A
80
76Jan07-26236C2
25% glycerol in LB
3E. coliTurbosL59#5O59-4pSBX1K3-BBa_I1350759KanFG1178ok#N/A#N/A
81
77Jan07-26238C2
25% glycerol in LB
3E. coliTurbosL61#3O61-2pSBX4A5-BBa_I1350761AmpFG1181bad sequencing#N/A#N/A
82
78Jan07-26239C2
25% glycerol in LB
3E. coliTurbosL62#3O62-2pSBX4C5-BBa_I1350762Cm#N/A#N/A
83
79Jan07-26240C2
25% glycerol in LB
3E. coliTurbosL63#3O63-2pSBX4K5-BBa_I1350763KanFG1171no sequenzing#N/A#N/A
84
80Jan07-26234C3
25% glycerol in LB
3E. coliTurbosL57#5O57-4pSBX1A3-BBa_I1350757AmpFG1174bad sequencing#N/A#N/A
85
81Jan07-26235C3
25% glycerol in LB
3E. coliTurbosL58#3O58-5pSBX1C3-BBa_I1350758CmFG1166bad sequenzing#N/A#N/A
86
82Jan07-26236C3
25% glycerol in LB
3E. coliTurbosL59#5O59-5pSBX1K3-BBa_I1350759KanFG1179ok, short read#N/A#N/A
87
83Jan07-26238C3
25% glycerol in LB
3E. coliTurbosL61#3O61-3pSBX4A5-BBa_I1350761AmpFG1182bad sequencing#N/A#N/A
88
84Jan07-26239C3
25% glycerol in LB
3E. coliTurbosL62#3O62-3pSBX4C5-BBa_I1350762Cm#N/A#N/A
89
85Jan07-26240C3
25% glycerol in LB
3E. coliTurbosL63#3O63-3pSBX4K5-BBa_I1350763KanFG1188bad sequencing#N/A#N/A
90
86Silvan07-31263C1-2
25% glycerol in LB
6E. coliBI21(DE3)O48pSB1AK8-T7-2ZNpu:(GFP-H6)48Amp#N/A
II2264, II2266
,
91
87Charlotte07-31279C1
25% glycerol in LB
3E. coliTurbosS80O80pSBX4C5-2ZSspH6:GFP80Cm#N/A#N/A
92
88Charlotte07-31280C1
25% glycerol in LB
3E. coliTurbosS81pSBX4C5-2ZNpuH6:GFP81Cm#N/A#N/A#N/A
93
89Caro08-02297C1
25% glycerol in LB
1E. coliTOP10S77O77pSBX1C3-T7RBS_BBv277Cm#N/A#N/A
94
90Caro08-02289C1
25% glycerol in LB
3E. coliTurbosS78O78pSBX1K3-T7RBS_BBv278Kan76DB35seems ok; some bases badly called#N/A#N/A
95
91Caro08-02289C2
25% glycerol in LB
3E. coliTurbosS78O78-2pSBX1K3-T7RBS_BBv278Kan#N/A#N/A
96
92Caro08-02289C3
25% glycerol in LB
3E. coliTurbosS78O78-3pSBX1K3-T7RBS_BBv278Kan#N/A#N/A
97
93Caro08-02285C1
25% glycerol in LB
3E. coliTurbosS72#2O72pSBX4A5-T7RBS-IGEMHD_v372AmpFG1191good sequencing, ok#N/A#N/A
98
94Caro08-02294C1
25% glycerol in LB
1E. coliTOP10S72#2O72-2pSBX4A5-T7RBS-IGEMHD_v372Amp#N/A#N/A
99
95Caro08-02292C1
25% glycerol in LB
1E. coliTOP10S70#2O70pSBX1K3-T7RBS-IGEMHD_v370Kan#N/A#N/A
100
96Caro08-02292C2
25% glycerol in LB
1E. coliTOP10S70#2O70-2pSBX1K3-T7RBS-IGEMHD_v370Kan#N/A#N/A
101
97Charlotte08-02299C1
25% glycerol in LB
6E. coliBI21(DE3)O80pSBX4C5-2ZSspH6:GFP80Cm#N/A
102
98Anna08-03242C13
25% glycerol in LB
3E. coliTurbosL60#3O60-3pSBX1T3-BBa_I1350760TetFG1180ok#N/A#N/A
103
99Anna08-03327C1
25% glycerol in LB
6E. coliBI21(DE3)O62O62-5pSBX4C5-BBa_I1350762Cmkeine Expression, wurde vernichtetFG1170ok
104
100Anna08-03327C2
25% glycerol in LB
6E. coliBI21(DE3)O62O62-6pSBX4C5-BBa_I1350762CmFunktionierender ExpressionsvektorFG1184ok, longestFG1170ok
105
101Anna08-03327C3
25% glycerol in LB
6E. coliBI21(DE3)O62O62-7pSBX4C5-BBa_I1350762Cmkeine Expression, wurde vernichtetFG1170ok
106
102Anna08-03327C4
25% glycerol in LB
6E. coliBI21(DE3)O62O62-8pSBX4C5-BBa_I1350762Cmkeine Expression, wurde vernichtetFG1170ok
107
103Anna08-03327C5
25% glycerol in LB
6E. coliBI21(DE3)O62O62-9pSBX4C5-BBa_I1350762CmFunktionierender ExpressionsvektorFG1185okFG1170ok
108
104Anna08-03327C6
25% glycerol in LB
6E. coliBI21(DE3)O62O62-10pSBX4C5-BBa_I1350762CmFunktionierender ExpressionsvektorFG1186okFG1170ok
109
105Jan08-12225C1
25% glycerol in LB
3E. coliTurbosD2014-4-8BpSB1T3-BBa_J0445064Tet#N/A#N/A#N/A
110
106Silvan08-18448C1
25% glycerol in LB
3E. coliTurbosO55-5pSB1A3-Smt3-2ZSortA-H6 (ccdB)55AmpII2281
111
107Silvan08-18448C2
25% glycerol in LB
3E. coliTurbosO55-6pSB1A3-Smt3-2ZSortA-H6 (ccdB)55AmpII2282
112
108Lisa08-05348C2
25% glycerol in LB
1E. coliTOP10O45-5O45-8pSB1C3-2ZNpuH6:GFP45Cm
II2257, II2256
no sequencing, we realized that this is npu (O45), has a mutation A1197del
113
109Lisa08-05349C1
25% glycerol in LB
1E. coliTOP10O36O36-3pSB1AK8-BBa_I71207436Amp, KanII2226
RFC23 (missing the final G in the prefix and the primary T in the suffix)
114
110Lisa08-05347C2
25% glycerol in LB
1E. coliTOP10O44-4O44-8pSB1C3-2ZSspH6:GFP44Cm
II2255, II2254
no sequencing, we realized that "ist glaube ich Ssp_DnaE. hat funktioniert", besides that ok
115
111Jan08-05356C1
25% glycerol in LB
6E. coliBI21(DE3)O17pHYRSF117Kanwas standing on the bench for a day#N/AII2232ok
116
112Jan08-05357C1
25% glycerol in LB
6E. coliBI21(DE3)O18pHYRSF1-0218Kanwas standing on the bench for a day#N/AII2233ok
117
113Jan08-05358C1
25% glycerol in LB
6E. coliBI21(DE3)O29pVS4129Ampwas standing on the bench for a day#N/AII2241ok
118
114Jan08-05359C1
25% glycerol in LB
6E. coliBI21(DE3)O30pVS0730Kanwas standing on the bench for a day#N/AII2242ok
119
115Jan08-05360C1
25% glycerol in LB
6E. coliBI21(DE3)O29pVS41, pHYRSF12917Amp, Kanwas standing on the bench for a day#N/AII2241ok
120
116Jan08-05361C1
25% glycerol in LB
6E. coliBI21(DE3)O29pVS41, pVS072930Amp, Kanwas standing on the bench for a day#N/AII2241ok
121
117
25% glycerol in LB
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
122
118
25% glycerol in LB
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
123
119
25% glycerol in LB
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
124
120
25% glycerol in LB
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
125
121
25% glycerol in LB
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
126
122
25% glycerol in LB
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
127
123
25% glycerol in LB
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
128
124
25% glycerol in LB
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
129
125
25% glycerol in LB
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
130
126
25% glycerol in LB
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
131
127
25% glycerol in LB
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
132
128
25% glycerol in LB
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
133
129
25% glycerol in LB
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
134
130
25% glycerol in LB
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
135
131
25% glycerol in LB
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
136
132
25% glycerol in LB
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
137
133Anna08-07384C1
25% glycerol in LB
6E. coliBI21(DE3)O59-3O59-6pSBX1K3-BBa_I1350759Kan
FG1167, FG1177, FG1199
frameshift, two fucking huge insertions, ok,longest,
138
134Anna08-07384C2
25% glycerol in LB
6E. coliBI21(DE3)O59-3O59-7pSBX1K3-BBa_I1350759Kannegativ
FG1167, FG1177, FG1199
frameshift, two fucking huge insertions, ok,longest,
139
135Anna08-07384C3
25% glycerol in LB
6E. coliBI21(DE3)O59-3O59-8pSBX1K3-BBa_I1350759Kan
FG1167, FG1177, FG1199
frameshift, two fucking huge insertions, ok,longest,
140
136Anna08-07385C1
25% glycerol in LB
6E. coliBI21(DE3)O59-5O59-9pSBX1K3-BBa_I1350759KanFG1179ok, short read
141
137Anna08-07385C2
25% glycerol in LB
6E. coliBI21(DE3)O59-5O59-10pSBX1K3-BBa_I1350759KanFG1179ok, short read
142
138Anna08-07385C3
25% glycerol in LB
6E. coliBI21(DE3)O59-5O59-11pSBX1K3-BBa_I1350759KanFG1179ok, short read
143
139Anna08-07386C1
25% glycerol in LB
6E. coliBI21(DE3)O61-4pSBX4A5-BBa_I1350761Ampnicht exprimierend#N/AFG1183ok
144
140Anna08-07386C2
25% glycerol in LB
6E. coliBI21(DE3)O61-4O61-5pSBX4A5-BBa_I1350761AmpFG1183ok
145
141Anna08-07386C3
25% glycerol in LB
6E. coliBI21(DE3)O61-4O61-6pSBX4A5-BBa_I1350761AmpFG1183ok
146
142Anna08-07387C1
25% glycerol in LB
6E. coliBI21(DE3)O63-4O63-5pSBX4K5-BBa_I1350763Kannicht exprimierendFG1189ok
147
143Anna08-07387C2
25% glycerol in LB
6E. coliBI21(DE3)O63-4pSBX4K5-BBa_I1350763Kan#N/AFG1189ok
148
144Anna08-07387C3
25% glycerol in LB
6E. coliBI21(DE3)O63-4O63-6pSBX4K5-BBa_I1350763KanFG1189ok
149
14808-10300C4
25% glycerol in LB
6E. coliBI21(DE3)O80-2O80-3pSBX4C5-2ZSspH6:GFP80Cm
FG1203, FG1208
ok, ok
150
14908-10301C3
25% glycerol in LB
6E. coliBI21(DE3)O81O81-2pSBX4C5-2ZNpuH6:GFP81Cm
FG1204, FG1209
pSBX4C5-2ZSspH6:GFP, pSBX4C5-2ZSspH6:GFP
151
15008-10372C4
25% glycerol in LB
6E. coliBI21(DE3)S86O86pSB1AK8-BBa_I712074-2ZSspH6:GFP86Amp, Kan
FG1205, FG1210
is just pSB1AK8-BBa_I712074, is just pSB1AK8-BBa_I712074
#N/A#N/A
152
15108-10351C7
25% glycerol in LB
6E. coliBI21(DE3)S87O87pSB1AK8-BBa_I712074-2ZNpuH6:GFP87Amp, Kan
FG1206, FG1211
mixture of pSB1AK8-T7-2ZNpu:(GFP-H6) and unknown, mixture of pSB1AK8-T7-2ZNpu:(GFP-H6) and unknown
#N/A#N/A
153
15208-13423C2
25% glycerol in LB
3E. coliTurbosO102-2O102-3pSBX1K3-T7RBS-LambdaLys-IGEMHD_v3102Kan
FG1200, II2274
ok, ok
154
15308-13423C3
25% glycerol in LB
3E. coliTurbosO102-2O102-4pSBX1K3-T7RBS-LambdaLys-IGEMHD_v3102Kan
FG1201, II2275
ok, ok
155
15408-13423C4
25% glycerol in LB
3E. coliTurbosO102-2O102-5pSBX1K3-T7RBS-LambdaLys-IGEMHD_v3102Kan
156
15508-13424C1
25% glycerol in LB
3E. coliTurbosA102O102-6pSBX1K3-T7RBS-LambdaLys-IGEMHD_v3102Kan#N/A#N/A
157
15608-13424C2
25% glycerol in LB
3E. coliTurbosA102O102-7pSBX1K3-T7RBS-LambdaLys-IGEMHD_v3102Kan
FG1202, II2276
ok, ok#N/A#N/A
158
15708-14433C1
25% glycerol in LB
3E. coliTurbosA103O103pSBX1K3-2Zv2Npu103Kancharlies zeug, falsch
FG1213, FG1214
wrong, charlie's stuff, wrong, charlie's stuff#N/A#N/A
159
15808-14434C1
25% glycerol in LB
3E. coliTurbosD2014-4-5CO104pSB1C3-BBa_I746908104CmsfGFP
II2277, II2278
ok, ok#N/A#N/A
160
15908-14433C4
25% glycerol in LB
3E. coliTurbosA103O103-2pSBX1K3-2Zv2Npu103Kan
II2279, II2280
ok (silent mutation in selection marker), ok (silent mutation in selection marker)
#N/A#N/A
161
16008-16436C1
25% glycerol in LB
6E. coliBI21(DE3)O102-3pSBX1K3-T7RBS-LambdaLys-IGEMHD_v3102Kan#N/A
FG1200, II2274
ok, ok
162
161Caro08-16Bsu1-1
25% glycerol in LB
8
Bacillus subtilis
168#N/A
163
16208-16442C2
25% glycerol in LB
3E. coliTurbosO73pSBX4C5-T7RBS-IGEMHD_v373Cm#N/AFG1192good sequencing, ok
164
16308-16442C2-1
25% glycerol in LB
3E. coliTurbosO73pSBX4C5-T7RBS-IGEMHD_v373Cm#N/AFG1192good sequencing, ok
165
16408-17438C8
25% glycerol in LB
3E. coliTurbosS98O98-3pSBX4C5-2Zv2Npu98Cmdiscard#N/A#N/A
166
16508-17438C9
25% glycerol in LB
3E. coliTurbosS98O98-4pSBX4C5-2Zv2Npu98CmII2273ok#N/A#N/A
167
16608-17441C1
25% glycerol in LB
3E. coliTurbosO71-2pSBX1T3-T7RBS-IGEMHD_v371Tet#N/AFG1190good sequencing, ok
168
16708-17441C1-1
25% glycerol in LB
3E. coliTurbosO71-2pSBX1T3-T7RBS-IGEMHD_v371Tet#N/AFG1190good sequencing, ok
169
16808-17443C1
25% glycerol in LB
3E. coliTurbosA103#2O103-3pSBX1K3-2Zv2Npu103Kantatsächlich richtig#N/A#N/A
170
1698/17/2014444C1
25% glycerol in LB
3E. coliTurbosG110O110pSBX1K3-2Zv2Npu:(LambdaLys-H6)110Kan#N/A#N/A
171
17008-17445C1
25% glycerol in LB
3E. coliTurbosG111O111pSBX1K3-2Zv2Npu:(LambdaLys-Rigid)111Kan#N/A#N/A
172
17108-17446C2
25% glycerol in LB
3E. coliTurbosG112O112pSBX1K3-2Zv2Npu:Xyla_BsaI_circ_His112Kan#N/A#N/A
173
17208-17447C1
25% glycerol in LB
3E. coliTurbosG113O113pSBX1K3-2Zv2Npu:Xyla_circ_noHis113Kan#N/A#N/A
174
173Silvan08-17448C3
25% glycerol in LB
3E. coliTurbosO55-7pSB1A3-Smt3-2ZSortA-H6 (ccdB)55AmpII2283
175
174Silvan08-17448C4
25% glycerol in LB
3E. coliTurbosO55-8pSB1A3-Smt3-2ZSortA-H6 (ccdB)55AmpII2284
176
175Silvan08-18453C3
25% glycerol in LB
3E. coliTurbosS116O116pSBX1K3-T7RBS-Smt3-2ZSortA-H6 (ccdB)116KanII2285#N/A#N/A
177
176Silvan08-18454C1
25% glycerol in LB
3E. coliTurbosS116#2O116-2pSBX1K3-T7RBS-Smt3-2ZSortA-H6 (ccdB)116KanII2286single base deletion#N/A#N/A
178
177Silvan08-18455C1
25% glycerol in LB
3E. coliTurbosS116#3O116-3pSBX1K3-T7RBS-Smt3-2ZSortA-H6 (ccdB)116KanII2287two-base deletion and point mutation#N/A#N/A
179
178Silvan08-18455C3
25% glycerol in LB
3E. coliTurbosS116#3O116-4pSBX1K3-T7RBS-Smt3-2ZSortA-H6 (ccdB)116Kan#N/A#N/A
180
179Lisa08-19458C1
25% glycerol in LB
3E. coliTurbosD2014-2-7EO109pSB1C3-BBa_K808000109CmII2288ok#N/A#N/A
181
180Lisa08-19458C2
25% glycerol in LB
3E. coliTurbosD2014-2-7EO109-2pSB1C3-BBa_K808000109Cmdiscard#N/A#N/A
182
181Lisa08-19458C3
25% glycerol in LB
3E. coliTurbosD2014-2-7EO109-3pSB1C3-BBa_K808000109Cmdiscard#N/A#N/A
183
182Lisa08-19467C1
25% glycerol in LB
3E. coliTurbos
D2013-2-17M
O117pSB1C3-BBa_K741002117Cmdiscard#N/A#N/A
184
183Lisa08-19467C2
25% glycerol in LB
3E. coliTurbos
D2013-2-17M
O117-2pSB1C3-BBa_K741002117CmII2289ok#N/A#N/A
185
184Lisa08-19467C3
25% glycerol in LB
3E. coliTurbos
D2013-2-17M
O117-3pSB1C3-BBa_K741002117Cmdiscard#N/A#N/A
186
185Anna08-20383C4
25% glycerol in LB
6E. coliBI21(DE3)O58-4pSBX1C3-BBa_I1350758Cm#N/AFG1175ok
187
186Anna08-20383C5
25% glycerol in LB
6E. coliBI21(DE3)O58-4pSBX1C3-BBa_I1350758Cm#N/AFG1175ok
188
187Anna08-20383C6
25% glycerol in LB
6E. coliBI21(DE3)O58-4pSBX1C3-BBa_I1350758Cm#N/AFG1175ok
189
188Anna08-20383C7
25% glycerol in LB
6E. coliBI21(DE3)O58-4pSBX1C3-BBa_I1350758Cm#N/AFG1175ok
190
189Anna08-20383C8
25% glycerol in LB
6E. coliBI21(DE3)O58-4pSBX1C3-BBa_I1350758Cm#N/AFG1175ok
191
190Anna08-20418C6
25% glycerol in LB
6E. coliBI21(DE3)O60-4pSBX1T3-BBa_I1350760Tet#N/A
192
191Anna08-20418C7
25% glycerol in LB
6E. coliBI21(DE3)O60-4pSBX1T3-BBa_I1350760Tet#N/A
193
192Anna08-20418C8
25% glycerol in LB
6E. coliBI21(DE3)O60-4pSBX1T3-BBa_I1350760Tet#N/A
194
193Anna08-20418C9
25% glycerol in LB
6E. coliBI21(DE3)O60-4pSBX1T3-BBa_I1350760Tet#N/A
195
194Anna08-20418C10
25% glycerol in LB
6E. coliBI21(DE3)O60-4pSBX1T3-BBa_I1350760Tet#N/A
196
195Anna08-20417C3
25% glycerol in LB
6E. coliBI21(DE3)O60-3pSBX1T3-BBa_I1350760Tetder einzige der richtig funktioniert#N/AFG1180ok
197
196MaxW08-21476C1
25% glycerol in LB
3E. coliTurbosG110#2O110-2pSBX1K3-2Zv2Npu:(LambdaLys-H6)110Kantaugt nix76DB28several deletions#N/A#N/A
198
197MaxW08-21476C2
25% glycerol in LB
3E. coliTurbosG110#2O110-3pSBX1K3-2Zv2Npu:(LambdaLys-H6)110Kan
76DB77, 76DB82
a deletion, a deletion#N/A#N/A
199
198MaxW08-21476C3
25% glycerol in LB
3E. coliTurbosG110#2pSBX1K3-2Zv2Npu:(LambdaLys-H6)110Kan#N/A#N/A#N/A
200
199Lisa08-22485C1
25% glycerol in LB
3E. coliTurbosA122O122pSBX1K3-1GateNpuDnaE(N)122Kan#N/A#N/A
201
200Lisa08-23486C1
25% glycerol in LB
3E. coliTurbosA123O123pSBX1K3-1GateNpuDnaE(C)123Kan
II2290, II2291
mutation A220G K74E in the mRFP CDS, besides ok, mutation A220G K74E in the mRFP CDS, besides ok
#N/A#N/A
202
201Lisa08-24486C2
25% glycerol in LB
3E. coliTurbosA123O123-2pSBX1K3-1GateNpuDnaE(C)123Kan#N/A#N/A
203
202MaxW8/23/2014501C1
25% glycerol in LB
3E. coliTurbosG111#2pSBX1K3-2Zv2Npu:(LambdaLys-Rigid)111Kan#N/A#N/A#N/A
204
203MaxW8/23/2014501C2
25% glycerol in LB
3E. coliTurbosG111#2pSBX1K3-2Zv2Npu:(LambdaLys-Rigid)111Kan#N/A#N/A#N/A
205
204MaxW8/23/2014501C3
25% glycerol in LB
3E. coliTurbosG111#2O111-2pSBX1K3-2Zv2Npu:(LambdaLys-Rigid)111Kan
II2294, II2295
EINE, 1, EINE fucking mutation........., EINE, 1, EINE fucking mutation.........
#N/A#N/A
206
205MaxW8/23/2014503C5
25% glycerol in LB
3E. coliTurbosG113#2O113-2pSBX1K3-2Zv2Npu:Xyla_circ_noHis113Kan
II2298, II2299
fürn arsch, fürn arsch#N/A#N/A
207
206MaxW8/23/2014502C6
25% glycerol in LB
3E. coliTurbosG112#2O112-2pSBX1K3-2Zv2Npu:Xyla_BsaI_circ_His112Kan
II2296, II2297
ok, ok#N/A#N/A
208
207MaxW8/23/2014502C7
25% glycerol in LB
3E. coliTurbosG112#2O112-3pSBX1K3-2Zv2Npu:Xyla_BsaI_circ_His112Kandiscard#N/A#N/A
209
208MaxW8/23/2014502C8
25% glycerol in LB
3E. coliTurbosG112#2O112-4pSBX1K3-2Zv2Npu:Xyla_BsaI_circ_His112Kandiscard#N/A#N/A
210
209MaxW8/23/2014504C6
25% glycerol in LB
3E. coliTurbosG124O124pSBX1K3-2Zv2Npu:Xyla(H6)destrCext124Kan
76DB73, 76DB78
insertions, insertions#N/A#N/A
211
210MaxW8/23/2014504C7
25% glycerol in LB
3E. coliTurbosG124O124-2pSBX1K3-2Zv2Npu:Xyla(H6)destrCext124Kan
II2300, II2301
fürn arsch, fürn arsch#N/A#N/A
212
211MaxW8/23/2014504C8
25% glycerol in LB
3E. coliTurbosG124O124-3pSBX1K3-2Zv2Npu:Xyla(H6)destrCext124Kan
76DB74, 76DB79
ok, ok#N/A#N/A
213
212MaxW8/23/2014504C9
25% glycerol in LB
3E. coliTurbosG124O124-4pSBX1K3-2Zv2Npu:Xyla(H6)destrCext124Kan#N/A#N/A
214
213MaxW8/23/2014512C5
25% glycerol in LB
3E. coliTurbosL125O125pSB1C3-Smt3-2ZSortA-H6 (ccdB)125Cm
76DB29, 76DB30
negative, contains GFP, negative, contains GFP
#N/A#N/A
215
214MaxW8/23/2014512C6
25% glycerol in LB
3E. coliTurbosL125O125-2pSB1C3-Smt3-2ZSortA-H6 (ccdB)125Cm
76DB31, 76DB32
negative, contains GFP, negative, contains GFP
#N/A#N/A
216
215MaxW8/23/2014512C7
25% glycerol in LB
3E. coliTurbosL125O125-3pSB1C3-Smt3-2ZSortA-H6 (ccdB)125Cm
76DB33, 76DB34
negative, contains GFP, negative, contains GFP
#N/A#N/A
217
216Charlotte8/24/2014500C2
25% glycerol in LB
6E. coliBI21(DE3)S86#5pSB1AK8-BBa_I712074-2ZSspH6:GFP86Amp, Kan#N/A#N/A#N/A
218
217Charlotte8/24/2014506C2
25% glycerol in LB
6E. coliBI21(DE3)S86#3pSB1AK8-BBa_I712074-2ZSspH6:GFP86Amp, Kan#N/A#N/A#N/A
219
218Charlotte8/24/2014510C2
25% glycerol in LB
6E. coliBI21(DE3)S86#5O86-3pSB1AK8-BBa_I712074-2ZSspH6:GFP86Amp, Kan
II2310, II2311
ok, ok#N/A#N/A
220
219Caro8/24/2014527C2
25% glycerol in LB
3E. coliTurbosA105O105pSBX1K3-T7RBS-H6-NpuDnaE^C-sfGFP^C-H6105Kandiscard#N/A#N/A
221
220Caro8/24/2014527C4
25% glycerol in LB
3E. coliTurbosA105O105-2pSBX1K3-T7RBS-H6-NpuDnaE^C-sfGFP^C-H6105Kan
II2312, II2313
ok, ok, C->T in VF2 site#N/A#N/A
222
221Caro8/24/2014528C1
25% glycerol in LB
3E. coliTurbosA106O106pSBX1K3-T7RBS-H6-NpuDnaE^C SN->AG-sfGFP^C-H6106Kan76DC22ok#N/A#N/A
223
222Caro8/24/2014528C3
25% glycerol in LB
3E. coliTurbosA106O106-2pSBX1K3-T7RBS-H6-NpuDnaE^C SN->AG-sfGFP^C-H6106Kan
II2314, II2315
mixture A64->T mutation, mixture A64->Tmutation
#N/A#N/A
224
223Caro8/24/2014529C1
25% glycerol in LB
3E. coliTurbosA107O107pSBX1K3-T7RBS-H6-sfGFP^N-NpuDnaE^N-H6107Kan
II2316, II2317
ok, good, ok, C->T in VF2 site#N/A#N/A
225
224Caro8/24/2014529C2
25% glycerol in LB
3E. coliTurbosA107O107-2pSBX1K3-T7RBS-H6-sfGFP^N-NpuDnaE^N-H6107Kandiscard#N/A#N/A
226
225Caro8/24/2014530C3
25% glycerol in LB
3E. coliTurbosA108O108pSBX1K3-T7RBS-H6-sfGFP^N-C->G NpuDnaE^N-H6108Kandiscard
II2318, II2319
1bp deletion before final H6, 1bp deletion in suffix, 1bp deletion before final H6, 1bp deletion in suffix
#N/A#N/A
227
226Caro8/24/2014530C2
25% glycerol in LB
3E. coliTurbosA108O108-2pSBX1K3-T7RBS-H6-sfGFP^N-C->G NpuDnaE^N-H6108Kan
76DC11, 76DC12
mixture with T7RBS T6del, mixture with T7RBS T6del
#N/A#N/A
228
227Silvan08-27544C1
25% glycerol in LB
3E. coliTurbosL125#2O125-4pSB1C3-Smt3-2ZSortA-H6 (ccdB)125Cm
76DB51, 76DB57
negative, contains GFP, negative, contains GFP
#N/A#N/A
229
228Silvan08-27544C2
25% glycerol in LB
3E. coliTurbosL125#2O125-5pSB1C3-Smt3-2ZSortA-H6 (ccdB)125Cm
76DB52, 76DB58
negative, contains GFP, negative, contains GFP
#N/A#N/A
230
229Silvan08-27544C3
25% glycerol in LB
3E. coliTurbosL125#2O125-6pSB1C3-Smt3-2ZSortA-H6 (ccdB)125Cm
76DB53, 76DB59
negative, contains GFP, negative, contains GFP
#N/A#N/A
231
230Silvan08-27544C4
25% glycerol in LB
3E. coliTurbosL125#2O125-7pSB1C3-Smt3-2ZSortA-H6 (ccdB)125Cm
76DB54, 76DB60
negative, contains GFP, negative, contains GFP
#N/A#N/A
232
231Silvan08-27544C5
25% glycerol in LB
3E. coliTurbosL125#2O125-8pSB1C3-Smt3-2ZSortA-H6 (ccdB)125Cm
76DB55, 76DB61
negative, contains GFP, negative, contains GFP
#N/A#N/A
233
232Silvan08-27544C6
25% glycerol in LB
3E. coliTurbosL125#2O125-9pSB1C3-Smt3-2ZSortA-H6 (ccdB)125Cm
76DB56, 76DB62
negative, contains GFP, negative, contains GFP
#N/A#N/A
234
233Jakob08-27525C1-1
25% glycerol in LB
3E. coliTurbosS94#4O94-9pSBX4C5-2GateNpuDnaE(N)94Cm#N/A#N/A
235
234Jakob08-27525C2-1
25% glycerol in LB
3E. coliTurbosS94#4O94-10pSBX4C5-2GateNpuDnaE(N)94Cm#N/A#N/A
236
235Jakob08-27526C1-1
25% glycerol in LB
3E. coliTurbosS95#4O95-9pSBX4C5-2GateNpuDnaE(C)95Cm#N/A#N/A
237
236Jakob08-27526C2-1
25% glycerol in LB
3E. coliTurbosS95#4O95-10pSBX4C5-2GateNpuDnaE(C)95Cm#N/A#N/A
238
237MaxW08-28543C3
25% glycerol in LB
3E. coliTurbosG131pSBX4C5-2Zv2Npu:LaLys+ccdb131Cm#N/A#N/A#N/A
239
238MaxW08-28543C5
25% glycerol in LB
3E. coliTurbosG131O131pSBX4C5-2Zv2Npu:LaLys+ccdb131Cm
76DB75, 76DB80
gfp..............................., gfp...............................
#N/A#N/A
240
239MaxW08-28543C7
25% glycerol in LB
3E. coliTurbosG131O131-2pSBX4C5-2Zv2Npu:LaLys+ccdb131Cm
76DB76, 76DB81
ccdb missing, ccdb missing#N/A#N/A
241
240Lisa08-28547C1
25% glycerol in LB
3E. coliTurbosA132O132pSB1C3-Plac-GFP-IntN-MTS132Cmdiscard
76DB83, 76DB84
is actually psbx ssp h6 gfp, is actually psbx ssp h6 gfp
#N/A#N/A
242
241Lisa08-28547C2
25% glycerol in LB
3E. coliTurbosA132O132-2pSB1C3-Plac-GFP-IntN-MTS132Cmdiscard#N/A#N/A
243
242Lisa08-28547C3
25% glycerol in LB
3E. coliTurbosA132O132-3pSB1C3-Plac-GFP-IntN-MTS132Cmdiscard#N/A#N/A
244
243Caro08-28552C2
25% glycerol in LB
6E. coliBI21(DE3)S129#3O129-2pSBX1K3-sfGFPN-NpuDnaEN_NpuDnaEC-sfGFPC129#N/A#N/A#N/A
245
244Caro08-28552C3
25% glycerol in LB
6E. coliBI21(DE3)S129#3O129-3pSBX1K3-sfGFPN-NpuDnaEN_NpuDnaEC-sfGFPC129#N/A#N/A#N/A
246
245Consti08-30562C1
25% glycerol in LB
3E. coliTurbosG113#3O113-3pSBX1K3-2Zv2Npu:Xyla_circ_noHis113Kan
76DC23, 76DC24
ok, ok#N/A#N/A
247
246Consti08-30562C4
25% glycerol in LB
3E. coliTurbosG113#3O113-4pSBX1K3-2Zv2Npu:Xyla_circ_noHis113Kan
76DB87, 76DB88
ok, ok#N/A#N/A
248
247Consti08-30563C1
25% glycerol in LB
6E. coliBI21(DE3)O112-2pSBX1K3-2Zv2Npu:Xyla_BsaI_circ_His112Kan#N/A
II2296, II2297
ok, ok
249
248Consti08-30563C2
25% glycerol in LB
6E. coliBI21(DE3)O112-2pSBX1K3-2Zv2Npu:Xyla_BsaI_circ_His112Kan#N/A
II2296, II2297
ok, ok
250
249MaxW09-01583C1
25% glycerol in LB
3E. coliTurbosL131#2O131-3pSBX4C5-2Zv2Npu:LaLys+ccdb131Cmred#N/A#N/A
251
250MaxW09-01583C2
25% glycerol in LB
3E. coliTurbosL131#2O131-4pSBX4C5-2Zv2Npu:LaLys+ccdb131Cm
76DC01, 76DC02
ok, ok#N/A#N/A
252
251MaxW09-01583C4
25% glycerol in LB
3E. coliTurbosL131#2O131-5pSBX4C5-2Zv2Npu:LaLys+ccdb131Cm#N/A#N/A
253
252MaxW09-01583C6
25% glycerol in LB
3E. coliTurbosL131#2O131-6pSBX4C5-2Zv2Npu:LaLys+ccdb131Cm#N/A#N/A
254
253MaxW09-01584C1
25% glycerol in LB
3E. coliTurbosL141O141pSBX1K3-2Zv2Npu:LaLys+ccdb141Kan#N/A#N/A
255
254MaxW09-01584C3
25% glycerol in LB
3E. coliTurbosL141O141-2pSBX1K3-2Zv2Npu:LaLys+ccdb141Kan
76DC03, 76DC04
ok, ok#N/A#N/A
256
255MaxW09-01584C5
25% glycerol in LB
3E. coliTurbosL141O141-3pSBX1K3-2Zv2Npu:LaLys+ccdb141Kan#N/A#N/A
257
256MaxW09-01585C4
25% glycerol in LB
3E. coliTurbosG131#2pSBX4C5-2Zv2Npu:LaLys+ccdb131Cmred#N/A#N/A#N/A
258
257MaxW09-01585C5
25% glycerol in LB
3E. coliTurbosG131#2O131-7pSBX4C5-2Zv2Npu:LaLys+ccdb131Cm
76DC05, 76DC06
ok, ok#N/A#N/A
259
258MaxW09-01585C6
25% glycerol in LB
3E. coliTurbosG131#2O131-8pSBX4C5-2Zv2Npu:LaLys+ccdb131Cm
76DC07, 76DC08
ok, ok#N/A#N/A
260
259MaxW09-01585C7
25% glycerol in LB
3E. coliTurbosG131#2O131-9pSBX4C5-2Zv2Npu:LaLys+ccdb131Cm#N/A#N/A
261
260MaxW09-01585C9
25% glycerol in LB
3E. coliTurbosG131#2O131-10pSBX4C5-2Zv2Npu:LaLys+ccdb131Cm#N/A#N/A
262
261MaxW09-01586C5
25% glycerol in LB
3E. coliTurbosG141O141-4pSBX1K3-2Zv2Npu:LaLys+ccdb141Kan#N/A#N/A
263
262MaxW09-01586C8
25% glycerol in LB
3E. coliTurbosG141O141-5pSBX1K3-2Zv2Npu:LaLys+ccdb141Kan#N/A#N/A
264
263MaxW09-01586C9
25% glycerol in LB
3E. coliTurbosG141O141-6pSBX1K3-2Zv2Npu:LaLys+ccdb141Kan
76DC09, 76DC10
mutation in ccdb (R->H), mutation in ccdb (R->H)
#N/A#N/A
265
264Jakob09-01564C3
25% glycerol in LB
3E. coliTurbosG137O137-4pSBX4C5-1Gate-NpuDnaE(N)-H6137Cm
76DC17, 76DC18
ok, ok#N/A#N/A
266
265Jakob09-01565C3
25% glycerol in LB
3E. coliTurbosG138O138-4pSBX4C5-H6-1GateNpuDnaE(C)138Cm
76DC20, 76DC21
ok, ok#N/A#N/A
267
266Anna09-01595C1
25% glycerol in LB
3E. coliTurbosS133O133pSB1AK8-BBa_I712074-BBa_I13507133Amp, Kan#N/A#N/A
268
267Anna09-01595C2
25% glycerol in LB
3E. coliTurbosS133O133-3pSB1AK8-BBa_I712074-BBa_I13507133Amp, Kan#N/A#N/A
269
268Anna09-01595C3
25% glycerol in LB
3E. coliTurbosS133O133-4pSB1AK8-BBa_I712074-BBa_I13507133Amp, Kan#N/A#N/A
270
269Anna09-01573C4
25% glycerol in LB
6E. coliBI21(DE3)O57-5pSBX1A3-BBa_I1350757Amp#N/A
271
270Anna09-01573C5
25% glycerol in LB
6E. coliBI21(DE3)O57-5pSBX1A3-BBa_I1350757Amp#N/A
272
271Anna09-01573C6
25% glycerol in LB
6E. coliBI21(DE3)O57-5pSBX1A3-BBa_I1350757Amp#N/A
273
272Anna09-01573C7
25% glycerol in LB
6E. coliBI21(DE3)O57-5pSBX1A3-BBa_I1350757Amp#N/A
274
273Lisa09-02567C2
25% glycerol in LB
3E. coliTurbosS140O140pSB1C3-T7RBS_SspDnaBC_Ext140Cm
76DB99, 76DC00
it has a his tag (the two plates were mixed up) -> juhu! intein protease ready,
#N/A#N/A
275
274Lisa09-02567C3
25% glycerol in LB
3E. coliTurbosS140O140-2pSB1C3-T7RBS_SspDnaBC_Ext140Cm#N/A#N/A
276
275Caro09-02601C1
25% glycerol in LB
6E. coliBI21(DE3)S130#5O130pSBX1K3-sfGFPN-NpuDnaEN-C->G_NpuDnaEC-sfGFPC-SN->AG130#N/A
BI2119, BI2120
, #N/A#N/A
277
276Caro09-02602C1
25% glycerol in LB
3E. coliTurbosS130#5O130-2pSBX1K3-sfGFPN-NpuDnaEN-C->G_NpuDnaEC-sfGFPC-SN->AG130#N/A#N/A#N/A
278
277MaxW09-02596C1
25% glycerol in LB
6E. coliBI21(DE3)O111-3pSBX1K3-2Zv2Npu:(LambdaLys-Rigid)111Kan#N/A
76BD93, 76BD94
ok, ok
279
278MaxW09-02596C2
25% glycerol in LB
6E. coliBI21(DE3)O111-3pSBX1K3-2Zv2Npu:(LambdaLys-Rigid)111Kan#N/A
76BD93, 76BD94
ok, ok
280
279MaxW09-02597C1
25% glycerol in LB
6E. coliBI21(DE3)O110-4pSBX1K3-2Zv2Npu:(LambdaLys-H6)110Kan#N/A
76BD97, 76BD98
ok, ok
281
280MaxW09-02597C2
25% glycerol in LB
6E. coliBI21(DE3)O110-4pSBX1K3-2Zv2Npu:(LambdaLys-H6)110Kan#N/A
76BD97, 76BD98
ok, ok
282
281MaxW09-02598C1
25% glycerol in LB
6E. coliBI21(DE3)O124-3pSBX1K3-2Zv2Npu:Xyla(H6)destrCext124Kan#N/A
76DB74, 76DB79
ok, ok
283
282MaxW09-02598C2
25% glycerol in LB
6E. coliBI21(DE3)O124-3pSBX1K3-2Zv2Npu:Xyla(H6)destrCext124Kan#N/A
76DB74, 76DB79
ok, ok
284
283MaxW09-04610C1
25% glycerol in LB
3E. coliTurbosA160O160pSBX1K3-T7RBS-FLAG-Smt3-2Zv2Npu160Kan
BI2115, BI2116
mutations in terminator(g->a), in mrfp (still red anyway), in smt3 (Q->R), mutations in terminator(g->a), in mrfp (still red anyway), in smt3 (Q->R)
#N/A#N/A
285
284MaxW09-04610C2
25% glycerol in LB
3E. coliTurbosA160O160-2pSBX1K3-T7RBS-FLAG-Smt3-2Zv2Npu160Kan
BI2117, BI2118
bad sequencing, auch eine in mrfp#N/A#N/A
286
285MaxW09-04610C3
25% glycerol in LB
3E. coliTurbosA160O160-3pSBX1K3-T7RBS-FLAG-Smt3-2Zv2Npu160Kan
BI2147, BI2148
insertion in smt3, silent in mfrp#N/A#N/A
287
286MaxW09-04610C4
25% glycerol in LB
3E. coliTurbosA160O160-4pSBX1K3-T7RBS-FLAG-Smt3-2Zv2Npu160Kan
BI2149, BI2150, AF9230
ok, ok (silent in mrfp), #N/A#N/A
288
287MaxW09-04611C1
25% glycerol in LB
6E. coliBI21(DE3)O113-3pSBX1K3-2Zv2Npu:Xyla_circ_noHis113Kan#N/A
76DC23, 76DC24
ok, ok
289
288MaxW09-04611C2
25% glycerol in LB
6E. coliBI21(DE3)O113-3pSBX1K3-2Zv2Npu:Xyla_circ_noHis113Kan#N/A
76DC23, 76DC24
ok, ok
290
289Lisa09-04609C1
25% glycerol in LB
3E. coliTurbosS159O159pSB1C3-K808000_T7RBS_+His_SspDnaBC_Ext159Cm#N/A#N/A
291
290Lisa09-04609C3
25% glycerol in LB
3E. coliTurbosS159O159-2pSB1C3-K808000_T7RBS_+His_SspDnaBC_Ext159Cm#N/A#N/A
292
291Lisa09-04609C5
25% glycerol in LB
3E. coliTurbosS159O159-3pSB1C3-K808000_T7RBS_+His_SspDnaBC_Ext159Cm
76DB85, 76DB86
ok, ok, juhu, one part of intein protease ready, you should ring bell
#N/A#N/A
293
292Caro9/4/2014612C1
25% glycerol in LB
3E. coliTurbosG145O145pSBX4C5-H6-mRFPn1-154-NpuN-H6145Cm
BI2121, BI2122
, #N/A#N/A
294
293Caro09-04612C2
25% glycerol in LB
3E. coliTurbosG145O145-2pSBX4C5-H6-mRFPn1-154-NpuN-H6145Cm#N/A#N/A
295
294Caro9/4/2014613C2
25% glycerol in LB
3E. coliTurbosG146O146pSBX4C5-H6-NpuC-mRFP155-225-H6146Cm
BI2123, BI2124
, #N/A#N/A
296
295Caro09-04613C3
25% glycerol in LB
3E. coliTurbosG146O146-2pSBX4C5-H6-NpuC-mRFP155-225-H6146Cm#N/A#N/A
297
296Caro9/4/2014614C1
25% glycerol in LB
3E. coliTurbosG147O147pSBX4C5-H6-mRFP1-168-NpuN-H6147Cm
BI2125, BI2126
, #N/A#N/A
298
297Caro09-04614C2
25% glycerol in LB
3E. coliTurbosG147O147-2pSBX4C5-H6-mRFP1-168-NpuN-H6147Cm#N/A#N/A
299
298Caro9/4/2014615C2
25% glycerol in LB
3E. coliTurbosG148O148pSBX4C5-H6-NpuC-mRFP169-225-H6148Cm
BI2127, BI2128
, #N/A#N/A
300
299Caro09-04615C3
25% glycerol in LB
3E. coliTurbosG148O148-2pSBX4C5-H6-NpuC-mRFP169-225-H6148Cm#N/A#N/A
301
300Caro9/4/2014616C2
25% glycerol in LB
3E. coliTurbosG149O149pSBX4C5-H6-RenLucN-NpuN-H6149Cm
BI2129, BI2130
, #N/A#N/A
302
301Caro09-04616C3
25% glycerol in LB
3E. coliTurbosG149O149-2pSBX4C5-H6-RenLucN-NpuN-H6149Cm#N/A#N/A
303
302Caro9/4/2014617C2
25% glycerol in LB
3E. coliTurbosG150O150pSBX4C5-H6-NpuC-RenLucC-H6150Cm
BI2131, BI2132
, #N/A#N/A
304
303Caro09-04617C4
25% glycerol in LB
3E. coliTurbosG150O150-2pSBX4C5-H6-NpuC-RenLucC-H6150Cm#N/A#N/A
305
304Caro9/4/2014618C2
25% glycerol in LB
3E. coliTurbosG151O151pSBX4C5-H6-FireLucN-NpuN-H6151Cm
BI2133, BI2134
, #N/A#N/A
306
305Caro09-04618C3
25% glycerol in LB
3E. coliTurbosG151O151-2pSBX4C5-H6-FireLucN-NpuN-H6151Cm#N/A#N/A
307
306Caro9/4/2014619C2
25% glycerol in LB
3E. coliTurbosG152O152pSBX4C5-H6-NpuC-FireLucC-H6152Cm
BI2135, BI2136
, #N/A#N/A
308
307Caro09-04619C3
25% glycerol in LB
3E. coliTurbosG152O152-2pSBX4C5-H6-NpuC-FireLucC-H6152Cm#N/A#N/A
309
308Caro9/4/2014620C2
25% glycerol in LB
3E. coliTurbosG153O153pSBX4C5-H6-mCherryN-NpuN-H6153Cm
BI2137, BI2138
, #N/A#N/A
310
309Caro09-04620C3
25% glycerol in LB
3E. coliTurbosG153O153-2pSBX4C5-H6-mCherryN-NpuN-H6153Cm#N/A#N/A
311
310Caro9/4/2014621C2
25% glycerol in LB
3E. coliTurbosG154O154pSBX4C5-H6-NpuC-mCherryC-H6154Cm
BI2139, BI2140
, #N/A#N/A
312
311Caro09-04621C3
25% glycerol in LB
3E. coliTurbosG154O154-2pSBX4C5-H6-NpuC-mCherryC-H6154Cm#N/A#N/A
313
312Caro9/4/2014622C1
25% glycerol in LB
3E. coliTurbosG155O155pSBX4C5-H6-GFPn1-64-NpuN-H6155Cm
BI2141, BI2142
, #N/A#N/A
314
313Caro09-04622C2
25% glycerol in LB
3E. coliTurbosG155O155-2pSBX4C5-H6-GFPn1-64-NpuN-H6155Cm#N/A#N/A
315
314Caro9/4/2014624C2
25% glycerol in LB
3E. coliTurbosG157O157pSBX4C5-H6-GFPn1-157-NpuN-H6157Cm
BI2143, BI2144
, #N/A#N/A
316
315Caro09-04624C2
25% glycerol in LB
3E. coliTurbosG157O157pSBX4C5-H6-GFPn1-157-NpuN-H6157Cm
BI2143, BI2144
, #N/A#N/A
317
316Caro9/4/2014625C1
25% glycerol in LB
3E. coliTurbosG158O158pSBX4C5-H6-GFPc158-238-NpuC-H6158Cm#N/A#N/A
318
317Caro09-04625C2
25% glycerol in LB
3E. coliTurbosG158O158-2pSBX4C5-H6-GFPc158-238-NpuC-H6158Cm#N/A#N/A
319
318CARO9/7/2014660C1
25% glycerol in LB
6E. coliBI21(DE3)O105-2pSBX1K3-T7RBS-H6-NpuDnaE^C-sfGFP^C-H6105Kan#N/A
II2312, II2313
ok, ok, C->T in VF2 site
320
319CARO9/7/2014661C1
25% glycerol in LB
6E. coliBI21(DE3)O106pSBX1K3-T7RBS-H6-NpuDnaE^C SN->AG-sfGFP^C-H6106Kan#N/A76DC22ok
321
320CARO9/7/2014662C1
25% glycerol in LB
6E. coliBI21(DE3)O107pSBX1K3-T7RBS-H6-sfGFP^N-NpuDnaE^N-H6107Kan#N/A
II2316, II2317
ok, good, ok, C->T in VF2 site
322
321CARO9/7/2014663C1
25% glycerol in LB
6E. coliBI21(DE3)O108-2pSBX1K3-T7RBS-H6-sfGFP^N-C->G NpuDnaE^N-H6108Kan#N/A
76DC11, 76DC12
mixture with T7RBS T6del, mixture with T7RBS T6del
323
322
25% glycerol in LB
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
324
323MaxW09-08646C1
25% glycerol in LB
3E. coliTurbosA163O163pSBX4C5-2GateSspDnaX-S11C163Cm
BI2145, BI2171
ok, ok#N/A#N/A
325
324MaxW09-08646C2
25% glycerol in LB
3E. coliTurbosA163O163-2pSBX4C5-2GateSspDnaX-S11C163Cm#N/A#N/A
326
325MaxW09-08646C3
25% glycerol in LB
3E. coliTurbosA163pSBX4C5-2GateSspDnaX-S11C163Cm#N/A#N/A#N/A
327
326MaxW09-08647C1
25% glycerol in LB
3E. coliTurbosA164O164pSBX4C5-2GateSspDnaX-S11C HN->AG164Cm
BI2172, BI2173
ok, ok#N/A#N/A
328
327MaxW09-08647C2
25% glycerol in LB
3E. coliTurbosA164O164-2pSBX4C5-2GateSspDnaX-S11C HN->AG164Cm#N/A#N/A
329
328MaxW09-08647C3
25% glycerol in LB
3E. coliTurbosA164pSBX4C5-2GateSspDnaX-S11C HN->AG164Cm#N/A#N/A#N/A
330
329MaxW09-08648C1
25% glycerol in LB
3E. coliTurbosA165O165pSBX4C5-2GateSspDnaX-S11N165Cm
BI2174, BI2175, BI1917
part of intein+mrfp not sequenced, part of intein+mrfp not sequenced, ok
#N/A#N/A
331
330MaxW09-08648C2
25% glycerol in LB
3E. coliTurbosA165O165-2pSBX4C5-2GateSspDnaX-S11N165Cm#N/A#N/A
332
331MaxW09-08648C3
25% glycerol in LB
3E. coliTurbosA165pSBX4C5-2GateSspDnaX-S11N165Cm#N/A#N/A#N/A
333
332MaxW09-08649C1
25% glycerol in LB
3E. coliTurbosA166O166pSBX4C5-2GateSspDnaX-S11N C->G166Cm
BI2176, BI2177, BI1918
part of intein+mrfp not sequenced, part of intein+mrfp not sequenced, ok
#N/A#N/A
334
333MaxW09-08649C2
25% glycerol in LB
3E. coliTurbosA166O166-2pSBX4C5-2GateSspDnaX-S11N C->G166Cm#N/A#N/A
335
334MaxW09-08649C3
25% glycerol in LB
3E. coliTurbosA166pSBX4C5-2GateSspDnaX-S11N C->G166Cm#N/A#N/A#N/A
336
335MaxW09-08650C1
25% glycerol in LB
3E. coliTurbosA167O167pSBX4C5-2GateSspGyrB-S11C167Cm
BI2178, BI2179
ok, ok#N/A#N/A
337
336MaxW09-08650C2
25% glycerol in LB
3E. coliTurbosA167O167-2pSBX4C5-2GateSspGyrB-S11C167Cm#N/A#N/A
338
337MaxW09-08650C3
25% glycerol in LB
3E. coliTurbosA167pSBX4C5-2GateSspGyrB-S11C167Cm#N/A#N/A#N/A
339
338MaxW09-08651C1
25% glycerol in LB
3E. coliTurbosA168O168pSBX4C5-2GateSspGyrB-S11C SN->AG168Cm
BI2180, BI2181
ok, ok#N/A#N/A
340
339MaxW09-08651C2
25% glycerol in LB
3E. coliTurbosA168O168-2pSBX4C5-2GateSspGyrB-S11C SN->AG168Cm#N/A#N/A
341
340MaxW09-08651C3
25% glycerol in LB
3E. coliTurbosA168pSBX4C5-2GateSspGyrB-S11C SN->AG168Cm#N/A#N/A#N/A
342
341MaxW09-08652C1
25% glycerol in LB
3E. coliTurbosA169O169pSBX4C5-2GateSspGyrB-S11N169Cm
BI2182, BI2183, BI1919
part of intein+mrfp not sequenced, part of intein+mrfp not sequenced, mutation in ccdb c->a (?), ok, only intein sequenzed
#N/A#N/A
343
342MaxW09-08652C2
25% glycerol in LB
3E. coliTurbosA169O169-2pSBX4C5-2GateSspGyrB-S11N169Cm
BI2198, BI2199
ok, ok (mut in ccdb E87*)#N/A#N/A
344
343MaxW09-08652C3
25% glycerol in LB
3E. coliTurbosA169pSBX4C5-2GateSspGyrB-S11N169Cm#N/A#N/A#N/A
345
344MaxW09-08654C1
25% glycerol in LB
3E. coliTurbosA171O171pSBX4C5-2GateTerDnaE3-S11C171Cm
BI2184, BI2185
ok, ok#N/A#N/A
346
345MaxW09-08654C2
25% glycerol in LB
3E. coliTurbosA171O171-2pSBX4C5-2GateTerDnaE3-S11C171Cm#N/A#N/A
347
346MaxW09-08654C3
25% glycerol in LB
3E. coliTurbosA171pSBX4C5-2GateTerDnaE3-S11C171Cm#N/A#N/A#N/A
348
347MaxW09-08655C2
25% glycerol in LB
3E. coliTurbosA172O172pSBX4C5-2GateTerDnaE3-S11C SN->AG172Cm
BI2186, BI2187
ok, ok#N/A#N/A
349
348MaxW09-08655C3
25% glycerol in LB
3E. coliTurbosA172O172-2pSBX4C5-2GateTerDnaE3-S11C SN->AG172Cm#N/A#N/A
350
349MaxW09-08656C1
25% glycerol in LB
3E. coliTurbosA173O173pSBX4C5-2GateTerDnaE3-S11N173Cm
BI2188, BI2189
mutation in lacO-reg g->t, part of mrfp+intein not sequenced, part of mrfp+intein not sequenced
#N/A#N/A
351
350MaxW09-08656C2
25% glycerol in LB
3E. coliTurbosA173O173-2pSBX4C5-2GateTerDnaE3-S11N173Cm
BI2200, BI2201, BI1921
ok, ok, ok#N/A#N/A
352
351MaxW09-08656C3
25% glycerol in LB
3E. coliTurbosA173pSBX4C5-2GateTerDnaE3-S11N173Cm#N/A#N/A#N/A
353
352MaxW09-08657C1
25% glycerol in LB
3E. coliTurbosA174O174pSBX4C5-2GateTerDnaE3-S11N C->G174Cm
BI2190, BI2191
no mutations, begins in ccdb --> not fully seq, mut. in ccdb ga->tc
#N/A#N/A
354
353MaxW09-08657C2
25% glycerol in LB
3E. coliTurbosA174O174-2pSBX4C5-2GateTerDnaE3-S11N C->G174Cm
BI2202, BI2203, BI1922
ok, ok, ok#N/A#N/A
355
354MaxW09-08657C3
25% glycerol in LB
3E. coliTurbosA174pSBX4C5-2GateTerDnaE3-S11N C->G174Cm#N/A#N/A#N/A
356
355MaxW09-08658C1
25% glycerol in LB
3E. coliTurbosA143O143pSBX4C5-2GateNpuDnaE(N)*143Cm
BI2192, BI2193
part of intein/mrfp not sequenced, part of intein/mrfp not sequenced
#N/A#N/A
357
356MaxW09-08658C2
25% glycerol in LB
3E. coliTurbosA143O143-2pSBX4C5-2GateNpuDnaE(N)*143Cm
BI2204, BI2205, BI1923
ok, ok, ok#N/A#N/A
358
357MaxW09-08658C3
25% glycerol in LB
3E. coliTurbosA143pSBX4C5-2GateNpuDnaE(N)*143Cm#N/A#N/A#N/A
359
358MaxW09-08659C1
25% glycerol in LB
3E. coliTurbosA144O144pSBX4C5-2GateNpuDnaE(C)*144Cm#N/A#N/A
360
359MaxW09-08659C2
25% glycerol in LB
3E. coliTurbosA144O144-2pSBX4C5-2GateNpuDnaE(C)*144Cm
BI2206, BI2207
ok, ok#N/A#N/A
361
360MaxW09-08659C3
25% glycerol in LB
3E. coliTurbosA144pSBX4C5-2GateNpuDnaE(C)*144Cm#N/A#N/A#N/A
362
361Caro09-08540C4
25% glycerol in LB
6E. coliBI21(DE3)O128pSBX1K3-T7RBS-H6-sfGFP-H6128Kan#N/A
76DB36, 76DB37
perfect read, mutation in vf2 binding site??, insertion bbetween rbs and atg, perfect read, mutation in vf2 binding site??, insertion bbetween rbs and atg
363
362Caro09-08667C1
25% glycerol in LB
3E. coliTurbosO122-3pSBX1K3-1GateNpuDnaE(N)122Kan#N/A
II2320, II2321
ok, perfect, ok, perfect
364
363Caro09-10668C1
25% glycerol in LB
3E. coliTurbosO123-5pSBX1K3-1GateNpuDnaE(C)123Kan#N/A
II2322, II2323
ok, perfect, ok, perfect
365
364Caro09-10688C1
25% glycerol in LB
3E. coliTurbosG179O180pSBX1K3-T7RBS-H6-sfGFP_T65C-H6180Kan
BI1915, BI1916
deletion in 1st Stop codon, deletion in 1st Stop codon
#N/A#N/A
366
365Caro09-10688C2
25% glycerol in LB
3E. coliTurbosG179pSBX1K3-T7RBS-H6-sfGFP_T65C-H6180Kan#N/A#N/A#N/A
367
366Caro09-10688C3
25% glycerol in LB
3E. coliTurbosG179pSBX1K3-T7RBS-H6-sfGFP_T65C-H6180Kan#N/A#N/A#N/A
368
367MaxW09-10684C6
25% glycerol in LB
3E. coliTurbosA170#2O170pSBX4C5-2GateSspGyrB-S11N C->G170Cm
BI2208, BI2209, BI1920
ok, ok, ok#N/A#N/A
369
368MaxW09-10684C7
25% glycerol in LB
3E. coliTurbosA170#2O170-2pSBX4C5-2GateSspGyrB-S11N C->G170Cmdiscard#N/A#N/A
370
369MaxW09-10684C8
25% glycerol in LB
3E. coliTurbosA170#2O170-3pSBX4C5-2GateSspGyrB-S11N C->G170Cmdiscard#N/A#N/A
371
370MaxW09-10685C6
25% glycerol in LB
3E. coliTurbosA135b#2O135pSBX4C5-2GateGp41-1(N)135Cm
BI2210, BI2211, BI1924
ok, ok, ok#N/A#N/A
372
371MaxW09-10685C7
25% glycerol in LB
3E. coliTurbosA135b#2O135-2pSBX4C5-2GateGp41-1(N)135Cmdiscard#N/A#N/A
373
372MaxW09-10685C8
25% glycerol in LB
3E. coliTurbosA135b#2O135-3pSBX4C5-2GateGp41-1(N)135Cmdiscard#N/A#N/A
374
373MaxW09-10686C6
25% glycerol in LB
3E. coliTurbosG179O136pSBX4C5-2GateGp41-1(C)136Cm
BI2212, BI2213
ok, ok#N/A#N/A
375
374MaxW09-10686C7
25% glycerol in LB
3E. coliTurbosG179O136-2pSBX4C5-2GateGp41-1(C)136Cmdiscard#N/A#N/A
376
375MaxW09-10686C8
25% glycerol in LB
3E. coliTurbosG179O136-3pSBX4C5-2GateGp41-1(C)136Cmdiscard#N/A#N/A
377
376Consti09-11692C1
25% glycerol in LB
3E. coliTurbosS181O181pSBX1K3-Xylanase181KanBI1947ok#N/A#N/A
378
377Consti09-11692C2
25% glycerol in LB
3E. coliTurbosS181O181-2pSBX1K3-Xylanase181Kandiscard#N/A#N/A
379
378Consti09-11692C3
25% glycerol in LB
3E. coliTurbosS181O181-3pSBX1K3-Xylanase181KanBI12214ok#N/A#N/A
380
379Consti09-11692C4
25% glycerol in LB
3E. coliTurbosS181O181-4pSBX1K3-Xylanase181Kandiscard#N/A#N/A
381
380Consti09-11692C5
25% glycerol in LB
3E. coliTurbosS181O181-5pSBX1K3-Xylanase181Kandiscard#N/A#N/A
382
381Silvan09-11544C8-1
25% glycerol in H2O
3E. coliTurbosL125#2O125-10pSB1C3-Smt3-2ZSortA-H6 (ccdB)125Cm
BI1925, BI1932
, #N/A#N/A
383
382Silvan09-11676C1
25% glycerol in H2O
3E. coliTurbosA178O178pSB1C3-H6-0ZSortA178Cm
BI1929, BI1936, AG3767
ok, no result, ok#N/A#N/A
384
383Silvan09-11676C2
25% glycerol in H2O
3E. coliTurbosA178O178-2pSB1C3-H6-0ZSortA178Cm
BI1930, BI1937, AG3768
ok, no result, ok#N/A#N/A
385
384Silvan09-11676C3
25% glycerol in H2O
3E. coliTurbosA178O178-3pSB1C3-H6-0ZSortA178Cm
BI1931, BI1938, AG3769
H9R mutation, no result, ok#N/A#N/A
386
385Silvan09-11675C1-1
25% glycerol in H2O
3E. coliTurbosA177O177pSB1C3-2ZSortA-H6177Cm
BI1926, BI1933
, #N/A#N/A
387
386Silvan09-11675C2-1
25% glycerol in H2O
3E. coliTurbosA177O177-2pSB1C3-2ZSortA-H6177Cm
BI1927, BI1934
, #N/A#N/A
388
387Silvan09-11675C3-1
25% glycerol in H2O
3E. coliTurbosA177O177-3pSB1C3-2ZSortA-H6177Cm
BI1928, BI1935
, #N/A#N/A
389
388MaxW09-12705C1
25% glycerol in H2O
3E. coliTurbosG188O188pSBX1K3-LiLaLy(H6)188Kan
BI1939, BI1940
, luciferase??#N/A#N/A
390
389MaxW09-12705C2
25% glycerol in H2O
3E. coliTurbosG188O188-2pSBX1K3-LiLaLy(H6)188Kan#N/A#N/A
391
390MaxW09-12705C3
25% glycerol in H2O
3E. coliTurbosG188O188-3pSBX1K3-LiLaLy(H6)188Kan
BI1941, BI1942
, luciferase??#N/A#N/A
392
391MaxW09-12706C1
25% glycerol in H2O
3E. coliTurbosG189O189pSBX4C5-2Zv2Npu:LaLys+flexlinker189Cm
BI1943, BI1944
, is pSBX1K3-LiLaLy(H6)#N/A#N/A
393
392MaxW09-12706C2
25% glycerol in H2O
3E. coliTurbosG189O189-2pSBX4C5-2Zv2Npu:LaLys+flexlinker189Cm
BI1945, BI1946
, is pSBX1K3-LiLaLy(H6)#N/A#N/A
394
393MaxW09-12706C3
25% glycerol in H2O
3E. coliTurbosG189O189-3pSBX4C5-2Zv2Npu:LaLys+flexlinker189Cm#N/A#N/A
395
394Lisa09-12710C1
25% glycerol in H2O
3E. coliTurbosS161#5O161pSB1C3-BBa_K741002-Ter_F_KpnI-Ext_IntN_BamHI161CmBI1952hä?#N/A#N/A
396
395Lisa09-12710C2
25% glycerol in H2O
3E. coliTurbosS161#5O161-2pSB1C3-BBa_K741002-Ter_F_KpnI-Ext_IntN_BamHI161Cm#N/A#N/A
397
396Lisa09-12710C3
25% glycerol in H2O
3E. coliTurbosS161#5O161-3pSB1C3-BBa_K741002-Ter_F_KpnI-Ext_IntN_BamHI161Cm#N/A#N/A
398
397Caro09-12694C1
25% glycerol in H2O
3E. coliTurbosS182O182pSBX4C5-mRFPn1-154_mRFPc155-255_coex182Cm
BI1948, BI1949
ok, ok#N/A#N/A
399
398Caro09-12694C2
25% glycerol in H2O
3E. coliTurbosS182O182-2pSBX4C5-mRFPn1-154_mRFPc155-255_coex182Cm
BI1950, BI1951
ok, ok#N/A#N/A
400
399Caro09-12693C1
25% glycerol in H2O
6E. coliBI21(DE3)O180pSBX1K3-T7RBS-H6-sfGFP_T65C-H6180Kan#N/A
BI1915, BI1916
deletion in 1st Stop codon, deletion in 1st Stop codon
401
400Caro09-13700C1
25% glycerol in H2O
6E. coliBI21(DE3)O65-8pSB1C3-BBa_J0445065Cm#N/A
402
401Caro09-13701C1
25% glycerol in H2O
6E. coliBI21(DE3)O88pSB1C3-BBa_J5200888Cm#N/AFG1194ok
403
402Caro09-13702C1
25% glycerol in H2O
6E. coliBI21(DE3)O89pSB1AK8-BBa_I71201989Amp, Kan#N/AFG1195ok, good
404
403Caro09-13703C1
25% glycerol in H2O
6E. coliBI21(DE3)O90pSB1C3-BBa_J0670290Cm#N/AFG1196ok, good
405
404Caro09-13704C1
25% glycerol in H2O
6E. coliBI21(DE3)O91-2pSB1A2-BBa_E004091Amp#N/A
406
405Caro722C2???
25% glycerol in H2O
#N/A#N/A#N/AG201#N/A#N/A#N/A#N/A#N/A#N/A#N/A
407
406Caro722C3???
25% glycerol in H2O
#N/A#N/A#N/AG201#N/A#N/A#N/A#N/A#N/A#N/A#N/A
408
407Lisa09-15734C1
25% glycerol in H2O
3E. coliTurbosS207O207pSB1C3-EX-BsaI-CC-O-IasB-S207Cm#N/A#N/A
409
408Lisa09-15734C3
25% glycerol in H2O
3E. coliTurbosS207O207-2pSB1C3-EX-BsaI-CC-O-IasB-S207Cm
BI1953, BI1954
is O208-2......oo, 3.G afterprefix to AC, is O208-2......oo, 3.G afterprefix to AC
#N/A#N/A
410
409Lisa09-15735C1
25% glycerol in H2O
3E. coliTurbosS208O208pSB1C3-EX-BsaI-A-NN-IasB-S208Cm#N/A#N/A
411
410Lisa09-15735C3
25% glycerol in H2O
3E. coliTurbosS208O208-2pSB1C3-EX-BsaI-A-NN-IasB-S208Cm
BI1955, BI1956
is O207-2....:(, 3.G afterprefix to AC, is O207-2....:(, 3.G afterprefix to AC
#N/A#N/A
412
411Caro736C1
25% glycerol in H2O
O182-3pSBX4C5-mRFPn1-154_mRFPc155-255_coex182Cm#N/A
BI1972, BI1973
ok, ok
413
412Caro738C1
25% glycerol in H2O
6E. coliBI21(DE3)O183pSBX4C5-mRFPn1-168-mRFPc169-255_coex183Cm#N/A
BI1964, BI1965
ok, ok
414
413Caro740C1
25% glycerol in H2O
6E. coliBI21(DE3)O186pSBX4C5mCherryN_mCherryC_coex186Cm#N/A
BI1966, BI1967
ok, ok
415
414Caro744C2
25% glycerol in H2O
6E. coliBI21(DE3)O187pSBX4C5-GFPn1-157_GFPc158-255_coex187Cm#N/A
BI1968, BI1969
ok, ok
416
415Caro09-15781C1
25% glycerol in H2O
6E. coliBI21(DE3)O206pSB4C5-GFP_contr206Cm#N/A
BI1959, BI1960
ok, ok
417
416Caro09-15784C1
25% glycerol in H2O
6E. coliBI21(DE3)O205pSB4C5-mCherry_contr205Cm#N/A
BI1961, BI1962
ok, ok
418
417Charlotte09-16733C2
25% glycerol in H2O
6E. coliBI21(DE3)O189pSBX1K3-LiLaLy(H6)188Kan#N/A
BI1943, BI1944
, is pSBX1K3-LiLaLy(H6)
419
418MaxW09-17794C1
25% glycerol in H2O
3E. coliTurbosG189#2O189-4pSBX4C5-2Zv2Npu:LaLys+flexlinker189Cm
BI1974, BI1975
ok, bad seq#N/A#N/A
420
419MaxW09-17794C2
25% glycerol in H2O
3E. coliTurbosG189#2O189-5pSBX4C5-2Zv2Npu:LaLys+flexlinker189Cm
BI1976, BI1977
ok, ok#N/A#N/A
421
420MaxW09-17794C3
25% glycerol in H2O
3E. coliTurbosG189#2O189-6pSBX4C5-2Zv2Npu:LaLys+flexlinker189Cm#N/A#N/A
422
421MaxW09-17795C1
25% glycerol in H2O
3E. coliTurbosG216O216pSBX1K3-XylaH6BB216Kan#N/A#N/A
423
422MaxW09-17795C2
25% glycerol in H2O
3E. coliTurbosG216O216-2pSBX1K3-XylaH6BB216KanBI1978ok#N/A#N/A
424
423MaxW09-17795C3
25% glycerol in H2O
3E. coliTurbosG216O216-3pSBX1K3-XylaH6BB216Kan#N/A#N/A
425
424MaxW09-17796C1
25% glycerol in H2O
3E. coliTurbosG217O217pSBX1K3-XylaH6extBB217Kan
BI1979, BF8279
bad seq, bad seq#N/A#N/A
426
425MaxW09-17796C2
25% glycerol in H2O
3E. coliTurbosG217O217-2pSBX1K3-XylaH6extBB217KanBF8280ok#N/A#N/A
427
426MaxW09-17796C3
25% glycerol in H2O
3E. coliTurbosG217O217-3pSBX1K3-XylaH6extBB217Kan#N/A#N/A
428
427Caro09-17768C1
25% glycerol in H2O
1E. coliTOP10G192#2O192pSBX4C5-1GateNpuDnaE(N)*-H6192Cmto be discardedBI1980
looks like pSBX4C5-1GateNpuDnaEC_SN->AG_H6
#N/A#N/A
429
428Caro09-17769C1
25% glycerol in H2O
1E. coliTOP10G193#2O193pSBX4C5-1GateSspDnaX-S11(C)-H6193CmBI1982ok#N/A#N/A
430
429Caro09-17770C1
25% glycerol in H2O
1E. coliTOP10G194#2O194pSBX4C5-1GateSspDnaX-S11C HN->AG_H6194CmBI1984ok#N/A#N/A
431
430Caro09-17771C1
25% glycerol in H2O
1E. coliTOP10G195#2O195pSBX4C5-1GateSspDnaX-S11N_H6195CmBI1986ok#N/A#N/A
432
431Caro09-17772C1
25% glycerol in H2O
1E. coliTOP10G196#2O196pSBX4C5-1GateSspDnaX-S11N C->G_H6196CmBI1988ok#N/A#N/A
433
432Caro09-17773C1
25% glycerol in H2O
1E. coliTOP10G197#2O197pSBX4C5-1GateSspGyrB-S11C_H6197CmBI1990ok#N/A#N/A
434
433Caro09-17774C1
25% glycerol in H2O
1E. coliTOP10G198#2O198pSBX4C5-1GateSspGyrB-S11C SN->AG_H6198CmBI1992ok#N/A#N/A
435
434Caro09-17775C1
25% glycerol in H2O
1E. coliTOP10G199#2O199pSBX4C5-1GateSspGyrB-S11N_H6199CmBI1994ok#N/A#N/A
436
435Caro09-17776C1
25% glycerol in H2O
1E. coliTOP10G200#2O200pSBX4C5-1GateSspGyrB-S11N C->G_H6200CmBI1996ok#N/A#N/A
437
436Caro09-17777C1
25% glycerol in H2O
1E. coliTOP10G201#2O201pSBX4C5-1GateTerDnaE3-S11C_H6201CmBI1998ok#N/A#N/A
438
437Caro09-17778C1
25% glycerol in H2O
1E. coliTOP10G202#2O202pSBX4C5-1GateTerDnaE3-S11C SN->AG_H6202CmBI2000ok#N/A#N/A
439
438Caro09-17779C1
25% glycerol in H2O
1E. coliTOP10G203#2O203pSBX4C5-1GateTerDnaE3-S11(N)-H6203CmBI2002ok#N/A#N/A
440
439Caro09-17780C1
25% glycerol in H2O
1E. coliTOP10G204#2O204pSBX4C5-1GateTerDnaE3-S11(N)*-H6204CmBI2004ok#N/A#N/A
441
440Caro09-17811C1
25% glycerol in H2O
1E. coliTOP10G191#2O191pSBX4C5-1GateNpuDnaE(C)*-H6191CmBI2006
looks like pSBX4C5-1GateNpuDnaEN_C->G_H6
#N/A#N/A
442
441Caro09-17800C1
25% glycerol in H2O
6E. coliBI21(DE3)O146-3pSBX4C5-H6-NpuC-mRFP155-225-H6146Cm#N/A
BI2152, BI2164
ok,
443
442Caro09-17801C1
25% glycerol in H2O
6E. coliBI21(DE3)O147-3pSBX4C5-H6-mRFP1-168-NpuN-H6147Cm#N/A
BI2153, BI2165
kok,
444
443Caro09-17802C1
25% glycerol in H2O
6E. coliBI21(DE3)O148-3pSBX4C5-H6-NpuC-mRFP169-225-H6148Cm#N/ABI2154ok
445
444Caro09-17803C1
25% glycerol in H2O
6E. coliBI21(DE3)O149-3pSBX4C5-H6-RenLucN-NpuN-H6149Cm#N/A
BI2155, BI2166
ok, one read bad, silent mutation in ligation renlucN to NpuN,
446
445Caro09-17804C1
25% glycerol in H2O
6E. coliBI21(DE3)O151-3pSBX4C5-H6-FireLucN-NpuN-H6151Cm#N/A
BI2157, BI2167
ok,
447
446Caro09-17805C1
25% glycerol in H2O
6E. coliBI21(DE3)O152-3pSBX4C5-H6-NpuC-FireLucC-H6152Cm#N/ABI2158ok
448
447Caro09-17806C1
25% glycerol in H2O
6E. coliBI21(DE3)O153-3pSBX4C5-H6-mCherryN-NpuN-H6153Cm#N/A
BI2159, BI2168
ok,
449
448Caro09-17807C1
25% glycerol in H2O
6E. coliBI21(DE3)O154-3pSBX4C5-H6-NpuC-mCherryC-H6154Cm#N/A
76DB91, 76DB92
ok, ok
450
449Caro09-17808C1
25% glycerol in H2O
6E. coliBI21(DE3)O155-3pSBX4C5-H6-GFPn1-64-NpuN-H6155Cm#N/A
BI2160, BI2169
ok,
451
450Caro09-17809C1
25% glycerol in H2O
6E. coliBI21(DE3)O157-3pSBX4C5-H6-GFPn1-157-NpuN-H6157Cm#N/A
BI2161, BI2170
ok,
452
451Caro09-17810C1
25% glycerol in H2O
6E. coliBI21(DE3)O158-3pSBX4C5-H6-GFPc158-238-NpuC-H6158Cm#N/ABI2162ok, one shift after "stem loop"
453
452Caro09-17811C2
25% glycerol in H2O
1E. coliTOP10G191#2O191-2pSBX4C5-1GateNpuDnaE(C)*-H6191CmBI2008
looks like pSBX4C5-1GateNpuDnaEN_C->G_H6
#N/A#N/A
454
453Caro09-18800C1
25% glycerol in H2O
6E. coliBI21(DE3)O146-3pSBX4C5-H6-NpuC-mRFP155-225-H6146Cm#N/A
BI2152, BI2164
ok,
455
454Caro09-18801C1
25% glycerol in H2O
6E. coliBI21(DE3)O147-3pSBX4C5-H6-mRFP1-168-NpuN-H6147Cm#N/A
BI2153, BI2165
kok,
456
455Caro09-18802C1
25% glycerol in H2O
6E. coliBI21(DE3)O148-3pSBX4C5-H6-NpuC-mRFP169-225-H6148Cm#N/ABI2154ok
457
456Caro09-18803C1
25% glycerol in H2O
6E. coliBI21(DE3)O149-3pSBX4C5-H6-RenLucN-NpuN-H6149Cm#N/A
BI2155, BI2166
ok, one read bad, silent mutation in ligation renlucN to NpuN,
458
457Caro09-18804C1
25% glycerol in H2O
6E. coliBI21(DE3)O151-3pSBX4C5-H6-FireLucN-NpuN-H6151Cm#N/A
BI2157, BI2167
ok,
459
458Caro09-18805C1
25% glycerol in H2O
6E. coliBI21(DE3)O152-3pSBX4C5-H6-NpuC-FireLucC-H6152Cm#N/ABI2158ok
460
459Caro09-18806C1
25% glycerol in H2O
6E. coliBI21(DE3)O153-3pSBX4C5-H6-mCherryN-NpuN-H6153Cm#N/A
BI2159, BI2168
ok,
461
460Caro09-18807C1
25% glycerol in H2O
6E. coliBI21(DE3)O154-3pSBX4C5-H6-NpuC-mCherryC-H6154Cm#N/A
76DB91, 76DB92
ok, ok
462
461Caro09-18808C1
25% glycerol in H2O
6E. coliBI21(DE3)O155-3pSBX4C5-H6-GFPn1-64-NpuN-H6155Cm#N/A
BI2160, BI2169
ok,
463
462Caro09-18809C1
25% glycerol in H2O
6E. coliBI21(DE3)O157-3pSBX4C5-H6-GFPn1-157-NpuN-H6157Cm#N/A
BI2161, BI2170
ok,
464
463Caro09-18810C1
25% glycerol in H2O
6E. coliBI21(DE3)O158-3pSBX4C5-H6-GFPc158-238-NpuC-H6158Cm#N/ABI2162ok, one shift after "stem loop"
465
464Maggie09-18819C1
25% glycerol in H2O
3E. coliTurbosG209O209
pSBX1K3-T7RBS-FLAG-Smt3-2ZNpu:(CWE-mDNMT1(731-1602)-CGCA=BsmBI-ccdB-BsmBI=TGCT-H5SGRGK)
209Kan#N/A#N/A
466
465Maggie09-18819C2
25% glycerol in H2O
3E. coliTurbosG209O209-2
pSBX1K3-T7RBS-FLAG-Smt3-2ZNpu:(CWE-mDNMT1(731-1602)-CGCA=BsmBI-ccdB-BsmBI=TGCT-H5SGRGK)
209Kan#N/A#N/A
467
466Maggie09-18819C3
25% glycerol in H2O
3E. coliTurbosG209O209-3
pSBX1K3-T7RBS-FLAG-Smt3-2ZNpu:(CWE-mDNMT1(731-1602)-CGCA=BsmBI-ccdB-BsmBI=TGCT-H5SGRGK)
209Kan#N/A#N/A
468
467Maggie09-18819C4
25% glycerol in H2O
3E. coliTurbosG209O209-4
pSBX1K3-T7RBS-FLAG-Smt3-2ZNpu:(CWE-mDNMT1(731-1602)-CGCA=BsmBI-ccdB-BsmBI=TGCT-H5SGRGK)
209Kan
BI2012, IH6892
ok, ccdB missing#N/A#N/A
469
468Maggie09-18819C5
25% glycerol in H2O
3E. coliTurbosG209O209-5
pSBX1K3-T7RBS-FLAG-Smt3-2ZNpu:(CWE-mDNMT1(731-1602)-CGCA=BsmBI-ccdB-BsmBI=TGCT-H5SGRGK)
209Kan#N/A#N/A
470
469Maggie09-18819C6
25% glycerol in H2O
3E. coliTurbosG209O209-6
pSBX1K3-T7RBS-FLAG-Smt3-2ZNpu:(CWE-mDNMT1(731-1602)-CGCA=BsmBI-ccdB-BsmBI=TGCT-H5SGRGK)
209Kan
BI2013, IH6893
really is pSBX1K3-T7RBS-FLAG-Smt3-2Zv2Npu (source vector), really is pSBX1K3-T7RBS-FLAG-Smt3-2Zv2Npu (source vector)
#N/A#N/A
471
470Maggie09-18819C7
25% glycerol in H2O
3E. coliTurbosG209O209-7
pSBX1K3-T7RBS-FLAG-Smt3-2ZNpu:(CWE-mDNMT1(731-1602)-CGCA=BsmBI-ccdB-BsmBI=TGCT-H5SGRGK)
209Kan#N/A#N/A
472
471Maggie09-18819C8
25% glycerol in H2O
3E. coliTurbosG209O209-8
pSBX1K3-T7RBS-FLAG-Smt3-2ZNpu:(CWE-mDNMT1(731-1602)-CGCA=BsmBI-ccdB-BsmBI=TGCT-H5SGRGK)
209Kan
BI2014, IH6894
no result (concentration probably was too low), no result (concentration probably was too low)
#N/A#N/A
473
472Silvan09-18818C1
25% glycerol in H2O
3E. coliTurbosA176#2pSB1C3-H6-Smt3-0ZSortA176Cm#N/A#N/A#N/A
474
473Silvan09-18818C2
25% glycerol in H2O
3E. coliTurbosA176#2O176pSB1C3-H6-Smt3-0ZSortA176Cm
AG3764, IH6895
ok, ok#N/A#N/A
475
474Silvan09-18818C3
25% glycerol in H2O
3E. coliTurbosA176#2O176-2pSB1C3-H6-Smt3-0ZSortA176Cm
AG3765, IH6896
H6R mutation, ok#N/A#N/A
476
475Silvan09-18818C4
25% glycerol in H2O
3E. coliTurbosA176#2pSB1C3-H6-Smt3-0ZSortA176Cm#N/A#N/A#N/A
477
476Silvan09-18818C5
25% glycerol in H2O
3E. coliTurbosA176#2pSB1C3-H6-Smt3-0ZSortA176Cm#N/A#N/A#N/A
478
477Silvan09-18818C7
25% glycerol in H2O
3E. coliTurbosA176#2O176-3pSB1C3-H6-Smt3-0ZSortA176Cm
AG3766, IH6897
ok, ok#N/A#N/A
479
478Silvan09-18818C8
25% glycerol in H2O
3E. coliTurbosA176#2pSB1C3-H6-Smt3-0ZSortA176Cm#N/A#N/A#N/A
480
479Lisa09-19820C1
25% glycerol in H2O
3E. coliTurbosG210#2O210pSB1C3-BsaI-CC-O-IasB-Ssra210Cm#N/A#N/A
481
480Lisa09-19820C2
25% glycerol in H2O
3E. coliTurbosG210#2O210-2pSB1C3-BsaI-CC-O-IasB-Ssra210CmBl1963#N/A#N/A
482
481Lisa09-19820C3
25% glycerol in H2O
3E. coliTurbosG210#2O210-3pSB1C3-BsaI-CC-O-IasB-Ssra210Cm#N/A#N/A
483
482Lisa09-19821C1
25% glycerol in H2O
3E. coliTurbosG211#2O211pSB1C3-BsaI-CC-O-IasB-MTS211Cm#N/A#N/A
484
483Lisa09-19821C2
25% glycerol in H2O
3E. coliTurbosG211#2O211-2pSB1C3-BsaI-CC-O-IasB-MTS211CmAG3770#N/A#N/A
485
484Lisa09-19821C3
25% glycerol in H2O
3E. coliTurbosG211#2O211-3pSB1C3-BsaI-CC-O-IasB-MTS211Cm#N/A#N/A
486
485Lisa09-19822C1
25% glycerol in H2O
3E. coliTurbosG212#2O212pSB1C3-EX-BsaI-A-NN-IasB-myristoilation212Cm#N/A#N/A
487
486Lisa09-19822C2
25% glycerol in H2O
3E. coliTurbosG212#2O212-2pSB1C3-EX-BsaI-A-NN-IasB-myristoilation212CmAG3771#N/A#N/A
488
487Lisa09-19822C3
25% glycerol in H2O
3E. coliTurbosG212#2O212-3pSB1C3-EX-BsaI-A-NN-IasB-myristoilation212Cm#N/A#N/A
489
488Lisa09-19823C1
25% glycerol in H2O
3E. coliTurbosG213#2O213pSB1C3-EX-BsaI-A-NN-IasB-NLS213Cm#N/A#N/A
490
489Lisa09-19823C2
25% glycerol in H2O
3E. coliTurbosG213#2O213-2pSB1C3-EX-BsaI-A-NN-IasB-NLS213CmAG3772#N/A#N/A
491
490Lisa09-19823C3
25% glycerol in H2O
3E. coliTurbosG213#2O213-3pSB1C3-EX-BsaI-A-NN-IasB-NLS213Cm#N/A#N/A
492
491Lisa09-19824C1
25% glycerol in H2O
3E. coliTurbosG214#2O214pSB1C3-EX-BsaI-A-NN-IasB-pelB214Cm#N/A#N/A
493
492Lisa09-19824C2
25% glycerol in H2O
3E. coliTurbosG214#2O214-2pSB1C3-EX-BsaI-A-NN-IasB-pelB214CmAG3773#N/A#N/A
494
493Lisa09-19824C3
25% glycerol in H2O
3E. coliTurbosG214#2O214-3pSB1C3-EX-BsaI-A-NN-IasB-pelB214Cm#N/A#N/A
495
494Lisa09-19825C1
25% glycerol in H2O
3E. coliTurbosG215#2O215pSB1C3-EX-BsaI-A-NN-IasB-PKI_NES215Cm#N/A#N/A
496
495Lisa09-19825C2
25% glycerol in H2O
3E. coliTurbosG215#2O215-2pSB1C3-EX-BsaI-A-NN-IasB-PKI_NES215Cm#N/A#N/A
497
496Lisa09-19825C3
25% glycerol in H2O
3E. coliTurbosG215#2O215-3pSB1C3-EX-BsaI-A-NN-IasB-PKI_NES215Cm#N/A#N/A
498
497Lisa09-19826C1
25% glycerol in H2O
3E. coliTurbosD2012-2-22PO218pSB1A2-BBa_K103006218Amp#N/A#N/A
499
498Lisa09-19826C2
25% glycerol in H2O
3E. coliTurbosD2012-2-22PO218-2pSB1A2-BBa_K103006218Amp#N/A#N/A
500
499Lisa09-19826C3
25% glycerol in H2O
3E. coliTurbosD2012-2-22PO218-3pSB1A2-BBa_K103006218Amp#N/A#N/A
501
500Jakob09-20799C2
25% glycerol in H2O
6E. coliBI21(DE3)O145-3pSBX4C5-H6-mRFPn1-154-NpuN-H6145Cm#N/A
BI2151, BI2163
ok,
502
501Silvan09-20828C1
25% glycerol in H2O
7E. coli
Rosetta (DE3)
O179pSBX1K3-T7RBS-Smt3-2ZSortA:(mDNMT1(731-1602)-FLAG)-H6, pRARE1793.10E+01Kan, Cm#N/A
BI1957, AF9231
,
503
502MaxW09-20840C1
25% glycerol in H2O
6E. coliBI21(DE3)O181-3pSBX1K3-Xylanase181Kan#N/ABI12214ok
504
503MaxW09-20841C1
25% glycerol in H2O
6E. coliBI21(DE3)O216-2pSBX1K3-XylaH6BB216Kan#N/ABI1978ok
505
504MaxW09-20842C1
25% glycerol in H2O
6E. coliBI21(DE3)O217pSBX1K3-XylaH6extBB217Kan#N/A
BI1979, BF8279
bad seq, bad seq
506
505Caro09-21853C1
25% glycerol in H2O
6E. coliBI21(DE3)G228O228-2pSBX4C5-0GateTerDnaE3-S11N_mRFP1-168228CmBF8274mutation im prefix?#N/A#N/A
507
506Caro09-21856C2
25% glycerol in H2O
3E. coliTurbosG192#3pSBX4C5-1GateNpuDnaE(N)*-H6192Cm#N/A#N/A#N/A
508
507Caro09-21857C2
25% glycerol in H2O
3E. coliTurbosG191#3O191-3pSBX4C5-1GateNpuDnaE(C)*-H6191CmBF8277ok short sequencing#N/A#N/A
509
508Caro09-21843C1
25% glycerol in H2O
3E. coliTurbosP322O220pL157(pLexA-MBP-VP16-mCherry-NES-wtAsLOV2-NESmodular)_C141T220Neo/Kan
BF8261, BF8262, BF8281, BF8282
, , good, but wrong -> no mutation, good, but wrong -> no mutation
510
509Caro09-21844C1
25% glycerol in H2O
3E. coliTurbosP322-2O220-2pL157(pLexA-MBP-VP16-mCherry-NES-wtAsLOV2-NESmodular)_C141T220Neo/Kan
BF8263, BF8264, BF8283, BF8284
, , ok, good!, ok, good!
511
510Caro09-21844C2
25% glycerol in H2O
3E. coliTurbosP322-2O220-3pL157(pLexA-MBP-VP16-mCherry-NES-wtAsLOV2-NESmodular)_C141T220Neo/Kan
BF8265, BF8266, BF8285, BF8286
, , ok, good!, ok, good!
512
511Caro09-22872C1
25% glycerol in H2O
6E. coliBI21(DE3)O228O228-4pSBX4C5-0GateTerDnaE3-S11N_mRFP1-168228CmBF8293ok, but one mutation in LacOperonBF8273
mutation im prefix? but less mutation than O228-2,use O228-4
513
512Caro09-22873C1
25% glycerol in H2O
6E. coliBI21(DE3)O229O229-2pSBX4C5-0GateNpuDnaE_C_mRFP169-225229CmBF8294ok, goodBF8275OK good
514
513MaxW09-24880C1
25% glycerol in H2O
3E. coliTurbosG244O244pSBX4C5-2Zv2Npu:LaLys+bad1244Cm28HD73ok#N/A#N/A
515
514MaxW09-24880C2
25% glycerol in H2O
3E. coliTurbosG244O244-2pSBX4C5-2Zv2Npu:LaLys+bad1244Cm#N/A#N/A
516
515MaxW09-24881C1
25% glycerol in H2O
3E. coliTurbosG245O245pSBX4C5-2Zv2Npu:LaLys+may1245Cm28HD74ok#N/A#N/A
517
516MaxW09-24881C2
25% glycerol in H2O
3E. coliTurbosG245O245-2pSBX4C5-2Zv2Npu:LaLys+may1245Cm#N/A#N/A
518
517MaxW09-24882C1
25% glycerol in H2O
3E. coliTurbosG246O246pSBX4C5-2Zv2Npu:LaLys+sgt1246Cm28HD75kacke#N/A#N/A
519
518MaxW09-24882C2
25% glycerol in H2O
3E. coliTurbosG246O246-2pSBX4C5-2Zv2Npu:LaLys+sgt1246Cm#N/A#N/A
520
519MaxW09-24883C1
25% glycerol in H2O
3E. coliTurbosG247O247pSBX4C5-2Zv2Npu:LaLys+sgt2247Cm28HD76kacke#N/A#N/A
521
520MaxW09-24883C2
25% glycerol in H2O
3E. coliTurbosG247O247-2pSBX4C5-2Zv2Npu:LaLys+sgt2247Cm#N/A#N/A
522
521MaxW09-24884C1
25% glycerol in H2O
3E. coliTurbosG248O248pSBX4C5-2Zv2Npu:LaLys+sho1248Cm28HD77ok#N/A#N/A
523
522MaxW09-24884C2
25% glycerol in H2O
3E. coliTurbosG248O248-2pSBX4C5-2Zv2Npu:LaLys+sho1248Cm#N/A#N/A
524
523MaxW09-24885C1
25% glycerol in H2O
3E. coliTurbosG249O249pSBX4C5-2Zv2Npu:LaLys+sho2249Cm28HD78ok#N/A#N/A
525
524MaxW09-24885C2
25% glycerol in H2O
3E. coliTurbosG249O249-2pSBX4C5-2Zv2Npu:LaLys+sho2249Cm#N/A#N/A
526
525Maggie09-24907C1
25% glycerol in H2O
3E. coliTurbosG232O232pSBX1K3-T7RBS-FLAG-Smt3-2ZNpu:(CWE-mDNMT1(731-1602)-H6SGRGT)232Kan
DD1384, DD1391
ok, ok#N/A#N/A
527
526Maggie09-24907C2
25% glycerol in H2O
3E. coliTurbosG232O232-2pSBX1K3-T7RBS-FLAG-Smt3-2ZNpu:(CWE-mDNMT1(731-1602)-H6SGRGT)232Kan
DD1385, DD1392
ok, frameshift mutation behind DNMT1#N/A#N/A
528
527Maggie09-24908C2
25% glycerol in H2O
3E. coliTurbosG231O231
pSBX1K3-T7RBS-Smt3-2ZSortA:(G2-mDNMT1(731-1602)-CGCA=BsaI-mRFPexp-BsaI=CTTC)-H6
231Kan
DD1386, DD1393
, #N/A#N/A
529
528Maggie09-24909C1
25% glycerol in H2O
1E. coliTOP10G231O231-2
pSBX1K3-T7RBS-Smt3-2ZSortA:(G2-mDNMT1(731-1602)-CGCA=BsaI-mRFPexp-BsaI=CTTC)-H6
231Kan#N/A#N/A
530
529Maggie09-24909C2
25% glycerol in H2O
1E. coliTOP10G231O231-3
pSBX1K3-T7RBS-Smt3-2ZSortA:(G2-mDNMT1(731-1602)-CGCA=BsaI-mRFPexp-BsaI=CTTC)-H6
231Kan#N/A#N/A
531
530Maggie09-24910C1
25% glycerol in H2O
3E. coliTurbosG239O239pSBX1K3-T7RBS-2ZSortA:eGFP-H6239Kan#N/A#N/A
532
531Maggie09-24910C2
25% glycerol in H2O
3E. coliTurbosG239O239-2pSBX1K3-T7RBS-2ZSortA:eGFP-H6239Kan28HE02ok#N/A#N/A
533
532Maggie09-24911C1
25% glycerol in H2O
1E. coliTOP10G239O239-3pSBX1K3-T7RBS-2ZSortA:eGFP-H6239Kan
DD1387, DD139428HE01
no result, no resultok#N/A#N/A
534
533Maggie09-24911C2
25% glycerol in H2O
1E. coliTOP10G239O239-4pSBX1K3-T7RBS-2ZSortA:eGFP-H6239Kan#N/A#N/A
535
534Maggie09-24912C2
25% glycerol in H2O
3E. coliTurbosG233pSBX1K3-T7RBS-Smt3-2ZSortA-H6 (mRFP)233Kan#N/A#N/A#N/A
536
535Maggie09-24913C1
25% glycerol in H2O
1E. coliTOP10G233O233-2pSBX1K3-T7RBS-Smt3-2ZSortA-H6 (mRFP)233Kan#N/A#N/A
537
536Maggie09-24913C2
25% glycerol in H2O
1E. coliTOP10G233O233-3pSBX1K3-T7RBS-Smt3-2ZSortA-H6 (mRFP)233Kan
DD1388, DD1395
no result, no result#N/A#N/A
538
537Maggie09-24913C3
25% glycerol in H2O
1E. coliTOP10G233O233-4pSBX1K3-T7RBS-Smt3-2ZSortA-H6 (mRFP)233Kan#N/A#N/A
539
538Maggie09-24917C2
25% glycerol in H2O
3E. coliTurbosG262O262pSBX1K3-T7RBS-FLAG-2ZNpu:(CWE-mDNMT1(731-1602)-mRFPexp-H5SGRGT)262Kan
DD1389, DD139628HE03, 28HE04
no result, no resultok, ok#N/A#N/A
540
539Maggie09-24917C3
25% glycerol in H2O
3E. coliTurbosG262O262-2pSBX1K3-T7RBS-FLAG-2ZNpu:(CWE-mDNMT1(731-1602)-mRFPexp-H5SGRGT)262Kan#N/A#N/A
541
540Maggie09-24917C4
25% glycerol in H2O
3E. coliTurbosG262O262-3pSBX1K3-T7RBS-FLAG-2ZNpu:(CWE-mDNMT1(731-1602)-mRFPexp-H5SGRGT)262Kan#N/A#N/A
542
541Maggie09-24917C5
25% glycerol in H2O
3E. coliTurbosG262O262-4pSBX1K3-T7RBS-FLAG-2ZNpu:(CWE-mDNMT1(731-1602)-mRFPexp-H5SGRGT)262Kan
DD1390, DD1397
no result, no result#N/A#N/A
543
542Consti09-25923C2
25% glycerol in H2O
6E. coliBI21(DE3)O217-2pSBX1K3-XylaH6extBB217Kan#N/ABF8280ok
544
543Silvan09-25931C2
25% glycerol in H2O
3E. coliTurbosG251#2O251pSBX1K3-T7RBS-Smt3-2ZSortA:(G4-mDNMT1(731-1602)-0917/1r)-H6251Kan28HD7996bp deletion#N/A#N/A
545
544Silvan09-25931C3
25% glycerol in H2O
3E. coliTurbosG251#2O251-2pSBX1K3-T7RBS-Smt3-2ZSortA:(G4-mDNMT1(731-1602)-0917/1r)-H6251Kan28HD8096bp deletion#N/A#N/A
546
545Silvan09-25932C1
25% glycerol in H2O
3E. coliTurbosG252#2O252pSBX1K3-T7RBS-Smt3-2ZSortA:(G4-mDNMT1(731-1602)-0915/6f-FLAG)-H6252Kan28HD81mixture#N/A#N/A
547
546Silvan09-25932C2
25% glycerol in H2O
3E. coliTurbosG252#2O252-2pSBX1K3-T7RBS-Smt3-2ZSortA:(G4-mDNMT1(731-1602)-0915/6f-FLAG)-H6252Kan28HD82
probably ok, but short read; possibly G->V mutation in linker
#N/A#N/A
548
547Silvan09-25933C1
25% glycerol in H2O
3E. coliTurbosG253#2O253pSBX1K3-T7RBS-Smt3-2ZSortA:(G4-mDNMT1(731-1602)-0915/4r-FLAG)-H6253Kan28HD83ok#N/A#N/A
549
548Silvan09-25933C2
25% glycerol in H2O
3E. coliTurbosG253#2O253-2pSBX1K3-T7RBS-Smt3-2ZSortA:(G4-mDNMT1(731-1602)-0915/4r-FLAG)-H6253Kan28HD8463bp deletion#N/A#N/A
550
549Silvan09-25934C1
25% glycerol in H2O
3E. coliTurbosS236O236pSBX1K3-T7RBS-H6-Smt3-0ZSortA236Kan
28HD85, 28HD97, 28HD98
wrong primer, ok; start of the read (backbone) looks a bit strange, ok
#N/A#N/A
551
550Silvan09-25934C2
25% glycerol in H2O
3E. coliTurbosS236O236-2pSBX1K3-T7RBS-H6-Smt3-0ZSortA236Kan28HD86wrong primer#N/A#N/A
552
551Silvan09-25935C1
25% glycerol in H2O
3E. coliTurbosS238O238pSBX1K3-T7RBS-H6-0ZSortA238Kan
28HD87, 28HD99, 28HE00
wrong primer, ok, ok#N/A#N/A
553
552Silvan09-25935C2
25% glycerol in H2O
3E. coliTurbosS238O238-2pSBX1K3-T7RBS-H6-0ZSortA238Kan28HD88wrong primer#N/A#N/A
554
553Silvan09-25936C1
25% glycerol in H2O
3E. coliTurbosS125O125-11pSB1C3-Smt3-2ZSortA-H6 (ccdB)125Cm
28HD89, 28HD90
ok, ok#N/A#N/A
555
554Silvan09-25936C2
25% glycerol in H2O
3E. coliTurbosS125O125-12pSB1C3-Smt3-2ZSortA-H6 (ccdB)125Cm#N/A#N/A
556
555Silvan09-25956C1
25% glycerol in H2O
3E. coliTurbosG265O265pSBX1K3-T7RBS-Smt3-2ZSortA:(G2-mDNMT1(731-1602)-0917/1r)-H6265Kan28HD91single base deletion#N/A#N/A
557
556Silvan09-25956C2
25% glycerol in H2O
3E. coliTurbosG265O265-2pSBX1K3-T7RBS-Smt3-2ZSortA:(G2-mDNMT1(731-1602)-0917/1r)-H6265Kan28HD92ok#N/A#N/A
558
557Silvan09-25956C3
25% glycerol in H2O
3E. coliTurbosG265O265-3pSBX1K3-T7RBS-Smt3-2ZSortA:(G2-mDNMT1(731-1602)-0917/1r)-H6265Kan#N/A#N/A
559
558Silvan09-25957C1
25% glycerol in H2O
3E. coliTurbosG250O250pSBX1K3-T7RBS-FLAG-2ZNpu:(CWE-mDNMT1(731-1602)-0917/2r-H6SGRGT)250Kan28HD94ok#N/A#N/A
560
559Silvan09-25957C2
25% glycerol in H2O
3E. coliTurbosG250O250-2pSBX1K3-T7RBS-FLAG-2ZNpu:(CWE-mDNMT1(731-1602)-0917/2r-H6SGRGT)250Kan28HD95single silent mutation#N/A#N/A
561
560Silvan09-25958C1
25% glycerol in H2O
3E. coliTurbosG254O254pSBX1K3-T7RBS-FLAG-2ZNpu:(CWE-mDNMT1(731-1602)-0915/3f-H6SGRGT)254Kan28HD96
sequence ok; signal intensity decreased after DNMT1
#N/A#N/A
562
561Silvan09-25958C2
25% glycerol in H2O
3E. coliTurbosG254O254-2pSBX1K3-T7RBS-FLAG-2ZNpu:(CWE-mDNMT1(731-1602)-0915/3f-H6SGRGT)254Kan28HD93ok#N/A#N/A
563
562Silvan09-25958C3
25% glycerol in H2O
3E. coliTurbosG254O254-3pSBX1K3-T7RBS-FLAG-2ZNpu:(CWE-mDNMT1(731-1602)-0915/3f-H6SGRGT)254Kan#N/A#N/A
564
563Jakob09-26964C1
25% glycerol in H2O
6E. coliBI21(DE3)O180-10pSBX1K3-T7RBS-H6-sfGFP_T65C-H6180Kan#N/ADD1410OK, mutagenesis worked
565
564Lisa09-26946C1
25% glycerol in H2O
3E. coliTurbosG263O263pSB1C3-EX-BsaI-A-NN-IasB-OmpA263Cm
28HE23, 28HE24
, #N/A#N/A
566
565Lisa09-26946C3
25% glycerol in H2O
3E. coliTurbosG263O263-2pSB1C3-EX-BsaI-A-NN-IasB-OmpA263Cm
DD1411, DD141228HE25, 28HE26
, ok, ok#N/A#N/A
567
566Caro09-26950C2
25% glycerol in H2O
6E. coliBI21(DE3)O224O224-4pSBX4C5-0GateSspDnaX-S11N_mRFP1-168224CmBF8268ok good
568
567Caro09-26950C3
25% glycerol in H2O
6E. coliBI21(DE3)O224O224-5pSBX4C5-0GateSspDnaX-S11N_mRFP1-168224CmBF8268ok good
569
568Caro09-26951C2
25% glycerol in H2O
6E. coliBI21(DE3)O225-3O225-4pSBX4C5-0GateSspGyrB-S11C_mRFP169-225225CmBF8288no mRFPc insert
570
569Caro09-26951C3
25% glycerol in H2O
6E. coliBI21(DE3)O225-3O225-5pSBX4C5-0GateSspGyrB-S11C_mRFP169-225225CmBF8288no mRFPc insert
571
570Caro09-26952C2
25% glycerol in H2O
6E. coliBI21(DE3)O226-4O226-5pSBX4C5-0GateSspGyrB-S11N_mRFP1-168226CmBF8290ok, good
572
571Caro09-26952C3
25% glycerol in H2O
6E. coliBI21(DE3)O226-4O226-6pSBX4C5-0GateSspGyrB-S11N_mRFP1-168226CmBF8290ok, good
573
572Caro09-26953C1
25% glycerol in H2O
6E. coliBI21(DE3)O230O230-3pSBX4C5-0GateNpuDnaE_N_mRFP1-168230CmBF8276mutation in mRFP G toD
574
573Caro09-26949C1
25% glycerol in H2O
6E. coliBI21(DE3)O223-2O223-5pSBX4C5-0GateSspDnaX-S11C_mRFP169-225223Cm
575
574MaxW09-27965C1
25% glycerol in H2O
3E. coliTurbosG284O284pSBX1K3-2Zv2Npu:LaLys+bad1284Kan28HE49no insert#N/A#N/A
576
575MaxW09-27965C2
25% glycerol in H2O
3E. coliTurbosG284O284-2pSBX1K3-2Zv2Npu:LaLys+bad1284Kan#N/A#N/A
577
576MaxW09-27966C1
25% glycerol in H2O
3E. coliTurbosG285O285pSBX1K3-2Zv2Npu:LaLys+may1285Kan28HE50ok#N/A#N/A
578
577MaxW09-27966C2
25% glycerol in H2O
3E. coliTurbosG285O285-2pSBX1K3-2Zv2Npu:LaLys+may1285Kan#N/A#N/A
579
578MaxW09-27967C1
25% glycerol in H2O
3E. coliTurbosG286O286pSBX1K3-2Zv2Npu:LaLys+sgt1286Kan28HE51no insert#N/A#N/A
580
579MaxW09-27967C2
25% glycerol in H2O
3E. coliTurbosG286O286-2pSBX1K3-2Zv2Npu:LaLys+sgt1286Kan#N/A#N/A
581
580MaxW09-27968C1
25% glycerol in H2O
3E. coliTurbosG287O287pSBX1K3-2Zv2Npu:LaLys+sgt2287Kan28HE52ok#N/A#N/A
582
581MaxW09-27968C2
25% glycerol in H2O
3E. coliTurbosG287O287-2pSBX1K3-2Zv2Npu:LaLys+sgt2287Kan#N/A#N/A
583
582MaxW09-27969C1
25% glycerol in H2O
3E. coliTurbosG288O288pSBX1K3-2Zv2Npu:LaLys+sho1288Kan28HE53ok#N/A#N/A
584
583MaxW09-27969C2
25% glycerol in H2O
3E. coliTurbosG288O288-2pSBX1K3-2Zv2Npu:LaLys+sho1288Kan#N/A#N/A
585
584MaxW09-27970C1
25% glycerol in H2O
3E. coliTurbosG289O289pSBX1K3-2Zv2Npu:LaLys+sho2289Kan28HE54ok#N/A#N/A
586
585MaxW09-27970C2
25% glycerol in H2O
3E. coliTurbosG289O289-2pSBX1K3-2Zv2Npu:LaLys+sho2289Kan#N/A#N/A
587
586MaxW09-27971C1
25% glycerol in H2O
3E. coliTurbosG290O290pSBX1K3-2Zv2Npu:LaLys+flexlinker290Kan28HE55ok#N/A#N/A
588
587MaxW09-27971C2
25% glycerol in H2O
3E. coliTurbosG290O290-2pSBX1K3-2Zv2Npu:LaLys+flexlinker290Kan#N/A#N/A
589
588Jan09-28929C3-2
25% glycerol in H2O
#N/A#N/A#N/Ap1680pSBX1K3-T7RBS-H6-sfGFP_T65C-H6180Kan#N/A#N/A#N/A
590
589Jan09-28838C1
25% glycerol in H2O
3E. coliTurbosO219pL157(pLexA-MBP-VP16-mCherry-NES-wtAsLOV2-NESmodular)219Neo/Kan#N/A
591
590Jan09-28961C1
25% glycerol in H2O
3E. coliTurbosP374pSBX4C5-2GateNpu*(C)_v2283Cm#N/A
592
591Jan09-28961C2
25% glycerol in H2O
3E. coliTurbosP374O283pSBX4C5-2GateNpu*(C)_v2283Cm
593
592Jan09-28961C3
25% glycerol in H2O
3E. coliTurbosP374O283-2pSBX4C5-2GateNpu*(C)_v2283Cm
594
593Jan09-28961C4
25% glycerol in H2O
3E. coliTurbosP374O283-3pSBX4C5-2GateNpu*(C)_v2283Cm
595
594Jan09-28961C5
25% glycerol in H2O
3E. coliTurbosP374pSBX4C5-2GateNpu*(C)_v2283Cm#N/A
596
595Caro/Jakob09-29983C1
25% glycerol in H2O
1E. coliTOP10A278O278pSBX1K3-DnaXN-sfGFPn-H6278KanDD1413ok one additional His#N/A#N/A
597
596Caro/Jakob09-29983C2
25% glycerol in H2O
1E. coliTOP10A278O278-2pSBX1K3-DnaXN-sfGFPn-H6278KanDD1414ok two additional His#N/A#N/A
598
597Caro/Jakob09-29983C3
25% glycerol in H2O
1E. coliTOP10A278O278-3pSBX1K3-DnaXN-sfGFPn-H6278Kan#N/A#N/A
599
598Caro/Jakob09-29984C1
25% glycerol in H2O
1E. coliTOP10A277O277pSBX1K3-DnaXN*-sfGFPn-H6277KanDD1415ok one additional His+#N/A#N/A
600
599Caro/Jakob09-29984C2
25% glycerol in H2O
1E. coliTOP10A277O277-2pSBX1K3-DnaXN*-sfGFPn-H6277KanDD1416okay#N/A#N/A
601
600Caro/Jakob09-29984C3
25% glycerol in H2O
1E. coliTOP10A277O277-3pSBX1K3-DnaXN*-sfGFPn-H6277Kan#N/A#N/A
602
601Caro/Jakob09-29985C1
25% glycerol in H2O
1E. coliTOP10A276O276pSBX1K3-SspDnaXc-sfGFP^C-H6276KanDD1417ok? two additional His#N/A#N/A
603
602Caro/Jakob09-29985C2
25% glycerol in H2O
1E. coliTOP10A276O276-2pSBX1K3-SspDnaXc-sfGFP^C-H6276KanDD1418do not use! bad mutations#N/A#N/A
604
603Caro/Jakob09-29985C3
25% glycerol in H2O
1E. coliTOP10A276O276-3pSBX1K3-SspDnaXc-sfGFP^C-H6276Kan#N/A#N/A
605
604Caro/Jakob09-29987C1
25% glycerol in H2O
1E. coliTOP10A279O279pSBX1K3-LOV-BsaIIasB-sfGFPc279Kanis most probably O281
DD1419, DD1435
could be O281?? checking with next sequencing, changed sequencing or construct, additional His, could be O281?? checking with next sequencing
#N/A#N/A
606
605Caro/Jakob09-29987C2
25% glycerol in H2O
1E. coliTOP10A279O279-2pSBX1K3-LOV-BsaIIasB-sfGFPc279Kanis most probably O281-2
DD1420, DD1436
could be O281?? checking with next sequencing, changed sequencing or construct, additional His, could be O281?? checking with next sequencing, changed sequencing or construct, additional His
#N/A#N/A
607
606Caro/Jakob09-29987C3
25% glycerol in H2O
1E. coliTOP10A279O279-3pSBX1K3-LOV-BsaIIasB-sfGFPc279Kan#N/A#N/A
608
607Caro/Jakob09-29988C1
25% glycerol in H2O
1E. coliTOP10A280O280pSBX1K3-LOV-12-DnaXC-sfGFPc280Kan
DD1421, DD1437
no LOV domain, no LOV domain#N/A#N/A
609
608Caro/Jakob09-29988C2
25% glycerol in H2O
1E. coliTOP10A280O280-2pSBX1K3-LOV-12-DnaXC-sfGFPc280Kan
DD1422, DD1438
one additional His, frame shift in the end, one additional His, frame shift in the end
#N/A#N/A
610
609Caro/Jakob09-29988C3
25% glycerol in H2O
1E. coliTOP10A280O280-3pSBX1K3-LOV-12-DnaXC-sfGFPc280Kan
28HE14, 28HE18
ok, good, ok, good#N/A#N/A
611
610Caro/Jakob09-29989C1
25% glycerol in H2O
1E. coliTOP10A281O281pSBX1K3-LOV-6-DnaXC-sfGFPc281Kanis most probably O279
DD1423, DD1439
could be O279?? checking with next sequencing, changed sequencing or construct, additional His, could be O279?? checking with next sequencing, changed sequencing or construct, additional His
#N/A#N/A
612
611Caro/Jakob09-29989C2
25% glycerol in H2O
1E. coliTOP10A281O281-2pSBX1K3-LOV-6-DnaXC-sfGFPc281Kanmost probably O279-2
DD1424, DD1440
could be O279?? checking with next sequencing, changed sequencing or construct, additional His, could be O279?? checking with next sequencing, changed sequencing or construct, additional His
#N/A#N/A
613
612Caro/Jakob09-29989C3
25% glycerol in H2O
1E. coliTOP10A281O281-3pSBX1K3-LOV-6-DnaXC-sfGFPc281Kan#N/A#N/A
614
613Caro/Jakob09-29990C1
25% glycerol in H2O
1E. coliTOP10A267O267pSBX1K3-H6-mRFPn-DnaXN-H6267KanDD1425
bad read at the beginning, probably frame shift mutations in between
#N/A#N/A
615
614Caro/Jakob09-29990C2
25% glycerol in H2O
1E. coliTOP10A267O267-2pSBX1K3-H6-mRFPn-DnaXN-H6267KanDD1426
turned out to be pSBX1K3-sfGFPn-NpuDnaEN
#N/A#N/A
616
615Caro/Jakob09-29990C3
25% glycerol in H2O
1E. coliTOP10A267O267-3pSBX1K3-H6-mRFPn-DnaXN-H6267Kan28HE15ok, good#N/A#N/A
617
616Caro/Jakob09-29991C1
25% glycerol in H2O
1E. coliTOP10A268O268pSBX1K3-H6-mRFPn-DnaXN*-H6268KanDD1427Intein DnaXN insert not included#N/A#N/A
618
617Caro/Jakob09-29991C2
25% glycerol in H2O
1E. coliTOP10A268O268-2pSBX1K3-H6-mRFPn-DnaXN*-H6268KanDD1428Intein DnaXN insert not included#N/A#N/A
619
618Caro/Jakob09-29991C3
25% glycerol in H2O
1E. coliTOP10A268O268-3pSBX1K3-H6-mRFPn-DnaXN*-H6268Kan28HE16ok, good#N/A#N/A
620
619Caro/Jakob09-29992C1
25% glycerol in H2O
1E. coliTOP10A269O269pSBX1K3-H6-LOV-BsaIIasB-mRFPc-H6269Kan
DD1429, DD1441
frame shift between His, #N/A#N/A
621
620Caro/Jakob09-29992C2
25% glycerol in H2O
1E. coliTOP10A269O269-2pSBX1K3-H6-LOV-BsaIIasB-mRFPc-H6269Kan
DD1430, DD1442
ok one additional His, #N/A#N/A
622
621Caro/Jakob09-29992C3
25% glycerol in H2O
1E. coliTOP10A269O269-3pSBX1K3-H6-LOV-BsaIIasB-mRFPc-H6269Kan#N/A#N/A
623
622Caro/Jakob09-29993C1
25% glycerol in H2O
1E. coliTOP10A270O270pSBX1K3-H6-LOV-12-DnaXC-mRFPc-H6270Kan
DD1431, DD1443
okay, #N/A#N/A
624
623Caro/Jakob09-29993C2
25% glycerol in H2O
1E. coliTOP10A270O270-2pSBX1K3-H6-LOV-12-DnaXC-mRFPc-H6270Kan
DD1432, DD1444
okay, one point mutation in Lac Operator, #N/A#N/A
625
624Caro/Jakob09-29993C3
25% glycerol in H2O
1E. coliTOP10A270O270-3pSBX1K3-H6-LOV-12-DnaXC-mRFPc-H6270Kan#N/A#N/A
626
625Caro/Jakob09-29994C1
25% glycerol in H2O
1E. coliTOP10A271O271pSBX1K3-H6-LOV-6-DnaXC-mRFPc-H6271Kan
DD1433, DD1445
6 amino acids missing in helixintein construct,
#N/A#N/A
627
626Caro/Jakob09-29994C2
25% glycerol in H2O
1E. coliTOP10A271O271-2pSBX1K3-H6-LOV-6-DnaXC-mRFPc-H6271Kan
DD1434, DD1446
LOV domain missing, #N/A#N/A
628
627Caro/Jakob09-29996C1
25% glycerol in H2O
1E. coliTOP10A273O273pSBX1K3-H6-DnaXC*-mRFPc-H6273Kan28HE71
is pSBX1K3-H6-DnaXC-mRFPc-H6, mutation H->Q ?, short read
#N/A#N/A
629
628Caro/Jakob09-29996C2
25% glycerol in H2O
1E. coliTOP10A273O273-2pSBX1K3-H6-DnaXC*-mRFPc-H6273Kan28HE72bad read, end of mRFP okay#N/A#N/A
630
629Caro/Jakob09-29996C3
25% glycerol in H2O
1E. coliTOP10A273O273-3pSBX1K3-H6-DnaXC*-mRFPc-H6273Kan28HE17many mutations#N/A#N/A
631
630Jan1072C1
25% glycerol in H2O
6E. coliBI21(DE3)G327O327pSBX4C5-1GateGp41-1(N)-H6327CmDD1447ok#N/A#N/A
632
631Jan1073C1
25% glycerol in H2O
6E. coliBI21(DE3)G328O328pSBX4C5-H6-1GateGp41-1(C)328CmDD1449ok#N/A#N/A
633
632Jan1074C1
25% glycerol in H2O
6E. coliBI21(DE3)G329O329pSBX4C5-DoubleGateGp41-1(N)329Cm
DD1451, DD1459
ok, not completely sequenced, ok, not completely sequenced
#N/A#N/A
634
633Jan1075C1
25% glycerol in H2O
6E. coliBI21(DE3)G330O330pSBX4C5-DoubleGateGp41-1(C)330Cm
DD1453, DD1461
ok, not completely sequenced, ok, not completely sequenced
#N/A#N/A
635
634Jan1076C1
25% glycerol in H2O
6E. coliBI21(DE3)G331O331pSBX4C5-DoubleNpuDnaE(N)331Cm
DD1455, DD1463
sequencing not long enough, poor sequenzing could be ok intein not sequenced
#N/A#N/A
636
635Jan1077C1
25% glycerol in H2O
6E. coliBI21(DE3)G332O332pSBX4C5-DoubleGateNpuDnaE(C)332Cm
DD1457, DD1465
sequencing not long enough, intein not sequenced, short sequenzin but ok
#N/A#N/A
637
636MaxW09-291006C1
25% glycerol in H2O
3E. coliTurbosG291O291pSBX1K3-2Zv2Npu:LaLys+bad2291Kan28HE56bad#N/A#N/A
638
637MaxW09-291006C2
25% glycerol in H2O
3E. coliTurbosG291O291-2pSBX1K3-2Zv2Npu:LaLys+bad2291Kan#N/A#N/A
639
638MaxW09-291007C1
25% glycerol in H2O
3E. coliTurbosG292O292pSBX1K3-2Zv2Npu:LaLys+bad3292Kan28HE57bad#N/A#N/A
640
639MaxW09-291007C2
25% glycerol in H2O
3E. coliTurbosG292O292-2pSBX1K3-2Zv2Npu:LaLys+bad3292Kan#N/A#N/A
641
640MaxW09-291008C1
25% glycerol in H2O
3E. coliTurbosG293O293pSBX1K3-2Zv2Npu:LaLys+bad4293Kan28HE58bad#N/A#N/A
642
641MaxW09-291008C2
25% glycerol in H2O
3E. coliTurbosG293O293-2pSBX1K3-2Zv2Npu:LaLys+bad4293Kan#N/A#N/A
643
642MaxW09-291009C1
25% glycerol in H2O
3E. coliTurbosG294O294pSBX1K3-2Zv2Npu:LaLys+may2294Kan28HE59no insert#N/A#N/A
644
643MaxW09-291009C2
25% glycerol in H2O
3E. coliTurbosG294O294-2pSBX1K3-2Zv2Npu:LaLys+may2294Kan#N/A#N/A
645
644MaxW09-291010C1
25% glycerol in H2O
3E. coliTurbosG295O295pSBX1K3-2Zv2Npu:LaLys+may3295Kan28HE60half insert#N/A#N/A
646
645MaxW09-291010C2
25% glycerol in H2O
3E. coliTurbosG295O295-2pSBX1K3-2Zv2Npu:LaLys+may3295Kan#N/A#N/A
647
646MaxW09-291011C1
25% glycerol in H2O
3E. coliTurbosG296O296pSBX1K3-2Zv2Npu:LaLys+ord1296Kan#N/A#N/A
648
647MaxW09-291011C2
25% glycerol in H2O
3E. coliTurbosG296O296-2pSBX1K3-2Zv2Npu:LaLys+ord1296Kan28HE61ok#N/A#N/A
649
648MaxW09-291012C1
25% glycerol in H2O
3E. coliTurbosG297O297pSBX1K3-2Zv2Npu:LaLys+ord2297Kan28HE62deletions#N/A#N/A
650
649MaxW09-291012C2
25% glycerol in H2O
3E. coliTurbosG297O297-2pSBX1K3-2Zv2Npu:LaLys+ord2297Kan#N/A#N/A
651
650MaxW09-291013C1
25% glycerol in H2O
3E. coliTurbosG298O298pSBX1K3-2Zv2Npu:LaLys+ord3298Kan28HE63ok#N/A#N/A
652
651MaxW09-291013C2
25% glycerol in H2O
3E. coliTurbosG298O298-2pSBX1K3-2Zv2Npu:LaLys+ord3298Kan#N/A#N/A
653
652MaxW09-291014C1
25% glycerol in H2O
3E. coliTurbosG299O299pSBX1K3-2Zv2Npu:LaLys+ord4299Kan28HE64half insert#N/A#N/A
654
653MaxW09-291014C2
25% glycerol in H2O
3E. coliTurbosG299O299-2pSBX1K3-2Zv2Npu:LaLys+ord4299Kan#N/A#N/A
655
654MaxW09-291015C1
25% glycerol in H2O
3E. coliTurbosG300O300pSBX1K3-2Zv2Npu:LaLys+ord5300Kan28HE65no insert#N/A#N/A
656
655MaxW09-291015C2
25% glycerol in H2O
3E. coliTurbosG300O300-2pSBX1K3-2Zv2Npu:LaLys+ord5300Kan#N/A#N/A
657
656MaxW09-291016C2
25% glycerol in H2O
3E. coliTurbosG301O301pSBX1K3-2Zv2Npu:LaLys+ord6301Kan28HE66no insert#N/A#N/A
658
657MaxW09-291016C3
25% glycerol in H2O
3E. coliTurbosG301O301-2pSBX1K3-2Zv2Npu:LaLys+ord6301Kan#N/A#N/A
659
658MaxW09-291017C1
25% glycerol in H2O
3E. coliTurbosG302O302pSBX1K3-2Zv2Npu:LaLys+sgt3302Kan28HE67no insert#N/A#N/A
660
659MaxW09-291017C2
25% glycerol in H2O
3E. coliTurbosG302O302-2pSBX1K3-2Zv2Npu:LaLys+sgt3302Kan#N/A#N/A
661
660MaxW09-291018C1
25% glycerol in H2O
3E. coliTurbosG303O303pSBX1K3-2Zv2Npu:LaLys+sgt4303Kan28HE68no insert#N/A#N/A
662
661MaxW09-291018C2
25% glycerol in H2O
3E. coliTurbosG303O303-2pSBX1K3-2Zv2Npu:LaLys+sgt4303Kan#N/A#N/A
663
662MaxW09-291019C1
25% glycerol in H2O
3E. coliTurbosG304O304pSBX1K3-2Zv2Npu:LaLys+sho3304Kan28HE69no insert#N/A#N/A
664
663MaxW09-291019C2
25% glycerol in H2O
3E. coliTurbosG304O304-2pSBX1K3-2Zv2Npu:LaLys+sho3304Kan#N/A#N/A
665
664MaxW09-291020C1
25% glycerol in H2O
3E. coliTurbosG305O305pSBX1K3-2Zv2Npu:LaLys+rigid_RGT305Kan28HE70insertions#N/A#N/A
666
665MaxW09-291020C2
25% glycerol in H2O
3E. coliTurbosG305O305-2pSBX1K3-2Zv2Npu:LaLys+rigid_RGT305Kan#N/A#N/A
667
666Schorly09-291044C1
25% glycerol in H2O
6E. coliBI21(DE3)O285pSBX1K3-2Zv2Npu:LaLys+may1285Kan#N/A28HE50ok
668
667Schorly09-291046C1
25% glycerol in H2O
6E. coliBI21(DE3)O287pSBX1K3-2Zv2Npu:LaLys+sgt2287Kan#N/A28HE52ok
669
668Schorly09-291047C1
25% glycerol in H2O
6E. coliBI21(DE3)O288pSBX1K3-2Zv2Npu:LaLys+sho1288Kan#N/A28HE53ok
670
669Schorly09-291048C1
25% glycerol in H2O
6E. coliBI21(DE3)O289pSBX1K3-2Zv2Npu:LaLys+sho2289Kan#N/A28HE54ok
671
670Schorly09-291049C1
25% glycerol in H2O
6E. coliBI21(DE3)O290pSBX1K3-2Zv2Npu:LaLys+flexlinker290Kan#N/A28HE55ok
672
671Konrad Herbst10-01KH38.2
25% glycerol in H2O
3E. coliTurbosO364-2364from Konrad Herbst
673
672Konrad Herbst10-01KH39PstI.1
25% glycerol in H2O
3E. coliTurbosO365-2365from Konrad Herbst
674
673Konrad Herbst10-01KH39.2
25% glycerol in H2O
3E. coliTurbosO366-2366from Konrad Herbst
675
674Jan1072C2
25% glycerol in H2O
6E. coliBI21(DE3)G327O327-2pSBX4C5-1GateGp41-1(N)-H6327CmDD1448will be repeated#N/A#N/A
676
675Jan1073C2
25% glycerol in H2O
6E. coliBI21(DE3)G328O328-2pSBX4C5-H6-1GateGp41-1(C)328CmDD1450ok#N/A#N/A
677
676Jan1074C2
25% glycerol in H2O
6E. coliBI21(DE3)G329O329-2pSBX4C5-DoubleGateGp41-1(N)329Cm
DD1452, DD1460
bad sequenzing, bad sequenzing#N/A#N/A
678
677Jan1075C2
25% glycerol in H2O
6E. coliBI21(DE3)G330O330-2pSBX4C5-DoubleGateGp41-1(C)330Cm
DD1454, DD1462
insertion in the prefix, mutation in mRFP, poor sequenzing, but ok
#N/A#N/A
679
678Jan1076C2
25% glycerol in H2O
6E. coliBI21(DE3)G331O331-2pSBX4C5-DoubleNpuDnaE(N)331Cm
DD1456, DD1464
sequencing not long enough, ok intein not sequenzed
#N/A#N/A
680
679Jan1077C2
25% glycerol in H2O
6E. coliBI21(DE3)G332O332-2pSBX4C5-DoubleGateNpuDnaE(C)332Cm
DD1458, DD1466
poor sequenzing, especially in the beginningg, but no indication that it was not ok, poor sequrnzing could be ok
#N/A#N/A
681
680Caro1066C1
25% glycerol in H2O
3E. coliTurbosG310O310pSBX4C5-0GateSspGyrB-S11C*_mRFP*169-225310Cmmost probably 308
28HE08, 28HE21
ok good, but not 310, DnaXC* inside (O308), ok, good but is actually DnaXC*-mRFPc (O308)
#N/A#N/A
682
681Caro1067C1
25% glycerol in H2O
3E. coliTurbosG311O311pSBX4C5-0GateSspGyrB-S11N*_mRFP*1-168311Cmmost probably 309
28HE09, 28HE35
ok good, but not 312 inside, DnaXN* inside (O309-2), ok, good, but actually DnaXN*-mRFPn (O309)
#N/A#N/A
683
682Caro1068C1
25% glycerol in H2O
3E. coliTurbosG312O312pSBX4C5-0GateTerDnaE3-S11C*_mRFP*169-225312Cmmost probably 310
28HE10, 28HE36
ok good, but GyrBC* (O310) inside , ok , good, but actually GyrBC*-mRFPc (O310)
#N/A#N/A
684
683Caro1069C1
25% glycerol in H2O
3E. coliTurbosG313O313pSBX4C5-0GateTerDnaE3-S11N*_mRFP*1-168313Cmmost probably 311
28HE11, 28HE37
ok good, but GyrBN* (O311) inside, ok, good, but actually GyrBN*-mRFPn (O311)
#N/A#N/A
685
684Caro1037C1
25% glycerol in H2O
3E. coliTurbosA272O272pSBX1K3-H6-DnaXC-mRFPc-H6272Kanmost probably 273
28HE44, 28HE19
actually pSBX1K3-H6-DnaXC*-mRFPc-H6, construct or sequencing changed?, ok good, but actually O273
#N/A#N/A
686
685Jan1072C3
25% glycerol in H2O
#N/A#N/A#N/AG327#N/A#N/A#N/A#N/A#N/A#N/A#N/A
687
686Jan1073C3
25% glycerol in H2O
#N/A#N/A#N/AG328#N/A#N/A#N/A#N/A#N/A#N/A#N/A
688
687Jan1074C3
25% glycerol in H2O
#N/A#N/A#N/AG329#N/A#N/A#N/A#N/A#N/A#N/A#N/A
689
688Jan1075C3
25% glycerol in H2O
#N/A#N/A#N/AG330#N/A#N/A#N/A#N/A#N/A#N/A#N/A
690
689Jan1076C3
25% glycerol in H2O
#N/A#N/A#N/AG331#N/A#N/A#N/A#N/A#N/A#N/A#N/A
691
690Jan1077C3
25% glycerol in H2O
#N/A#N/A#N/AG332#N/A#N/A#N/A#N/A#N/A#N/A#N/A
692
691Caro1063C2
25% glycerol in H2O
3E. coliTurbosG227#2O227-5pSBX4C5-0GateTerDnaE3-S11C_mRFP169-225227Cm28HE05good, ok#N/A#N/A
693
692Lisa10-031078C1
25% glycerol in H2O
3E. coliTurbosA240#5O240pSBX4C5-2GateSspDnaB(C)240CmDD1467ok#N/A#N/A
694
693Lisa10-031078C2
25% glycerol in H2O
3E. coliTurbosA240#5O240-2pSBX4C5-2GateSspDnaB(C)240Cm#N/A#N/A
695
694Lisa10-031078C3
25% glycerol in H2O
3E. coliTurbosA240#5O240-3pSBX4C5-2GateSspDnaB(C)240CmDD1468
ok (maybe there is something in the beginning of mRFP)
#N/A#N/A
696
695Lisa10-031079C1
25% glycerol in H2O
3E. coliTurbosA241#5O241pSBX4C5-2GateSspDnaB(C)*241Cm#N/A#N/A
697
696Lisa10-031079C2
25% glycerol in H2O
3E. coliTurbosA241#5O241-2pSBX4C5-2GateSspDnaB(C)*241CmDD1469ok#N/A#N/A
698
697Lisa10-031079C3
25% glycerol in H2O
3E. coliTurbosA241#5O241-3pSBX4C5-2GateSspDnaB(C)*241CmDD1470ok#N/A#N/A
699
698Lisa10-031080C1
25% glycerol in H2O
3E. coliTurbosA242#5O242pSBX4C5-2GateSspDnaB(N)242CmDD1471perfect mRFP read, call GATC#N/A#N/A
700
699Lisa10-031080C2
25% glycerol in H2O
3E. coliTurbosA242#5O242-2pSBX4C5-2GateSspDnaB(N)242CmDD1472perfect mRFP read, call GATC#N/A#N/A
701
700Lisa10-031080C3
25% glycerol in H2O
3E. coliTurbosA242#5O242-3pSBX4C5-2GateSspDnaB(N)242Cm#N/A#N/A
702
701Lisa10-031081C1
25% glycerol in H2O
3E. coliTurbosA243#5O243pSBX4C5-2GateSspDnaB(N)*243Cm#N/A#N/A
703
702Lisa10-031081C2
25% glycerol in H2O
3E. coliTurbosA243#5O243-2pSBX4C5-2GateSspDnaB(N)*243CmDD1473perfect mRFP read, call GATC#N/A#N/A
704
703Lisa10-031081C3
25% glycerol in H2O
3E. coliTurbosA243#5O243-3pSBX4C5-2GateSspDnaB(N)*243CmDD1474perfect mRFP read, call GATC#N/A#N/A
705
704Lisa10-031082C1
25% glycerol in H2O
3E. coliTurbosA325#2O325pSBX4C5-2GateSspDnaE(N)325CmDD1475, perfect mRFP read, call GATC, #N/A#N/A
706
705Lisa10-031082C2
25% glycerol in H2O
3E. coliTurbosA325#2O325-2pSBX4C5-2GateSspDnaE(N)325CmDD1476, perfect mRFP read, call GATC, #N/A#N/A
707
706Lisa10-031082C3
25% glycerol in H2O
3E. coliTurbosA325#2O325-3pSBX4C5-2GateSspDnaE(N)325Cm#N/A#N/A
708
707Lisa10-031083C1
25% glycerol in H2O
3E. coliTurbosA326#2O326pSBX4C5-2GateSspDnaE(C)326CmDD1477double insert insertion#N/A#N/A
709
708Lisa10-031083C2
25% glycerol in H2O
3E. coliTurbosA326#2O326-2pSBX4C5-2GateSspDnaE(C)326Cm#N/A#N/A
710
709Lisa10-031083C3
25% glycerol in H2O
3E. coliTurbosA326#2O326-3pSBX4C5-2GateSspDnaE(C)326CmDD1478tripple insert insertion#N/A#N/A
711
710Lisa10-031090C1
25% glycerol in H2O
3E. coliTurbosG346pSB1C3-EX-BsaI-CBD346Cm#N/A#N/A#N/A
712
711Lisa10-031090C2
25% glycerol in H2O
3E. coliTurbosG346O346pSB1C3-EX-BsaI-CBD346CmDD1479no.....GG could not work#N/A#N/A
713
712Lisa10-031090C3
25% glycerol in H2O
3E. coliTurbosG346pSB1C3-EX-BsaI-CBD346Cm#N/A#N/A#N/A
714
713Lisa10-031090C4
25% glycerol in H2O
3E. coliTurbosG346O346-2pSB1C3-EX-BsaI-CBD346CmDD1480no.....GG could not work#N/A#N/A
715
714Lisa10-031090C5
25% glycerol in H2O
3E. coliTurbosG346O346-3pSB1C3-EX-BsaI-CBD346Cm28HE43no.....GG could not work#N/A#N/A
716
715Charlottetoday1114C1
25% glycerol in H2O
6E. coliBI21(DE3)O296-2pSBX1K3-2Zv2Npu:LaLys+ord1296Kan#N/A28HE61ok
717
716Charlottetoday1115C1
25% glycerol in H2O
6E. coliBI21(DE3)O298pSBX1K3-2Zv2Npu:LaLys+ord3298Kan#N/A28HE63ok
718
717MaxW10-031097C1
25% glycerol in H2O
3E. coliTurbosS347O347pSB1C3-2Zv2Npu347Cm#N/A#N/A
719
718MaxW10-031098C1
25% glycerol in H2O
3E. coliTurbosS348O348pSB1C3-T7RBS-LambdaLys-IGEMHD_v3348Cm#N/A#N/A
720
719MaxW10-031099C1
25% glycerol in H2O
3E. coliTurbosS349O349pSB1C3-T7RBS-H6-NpuDnaE^C-sfGFP^C-H6349Cm#N/A#N/A
721
720MaxW10-031100C1
25% glycerol in H2O
3E. coliTurbosS350O350pSB1C3-T7RBS-H6-NpuDnaE^C SN->AG-sfGFP^C-H6350Cm#N/A#N/A
722
721MaxW10-031101C1
25% glycerol in H2O
3E. coliTurbosS351O351pSB1C3-T7RBS-H6-sfGFP^N-C->G NpuDnaE^N-H6351Cm#N/A#N/A
723
722MaxW10-031102C1
25% glycerol in H2O
3E. coliTurbosS352O352pSB1C3-2Zv2Npu:(LambdaLys-H6)352Cm#N/A#N/A
724
723MaxW10-031103C1
25% glycerol in H2O
3E. coliTurbosS353O353pSB1C3-2Zv2Npu:(LambdaLys-Rigid)353Cm#N/A#N/A
725
724MaxW10-031104C1
25% glycerol in H2O
3E. coliTurbosS354O354pSB1C3-2Zv2Npu:Xyla_BsaI_circ_His354Cm#N/A#N/A
726
725MaxW10-031105C2
25% glycerol in H2O
3E. coliTurbosS355O355pSB1C3-2Zv2Npu:Xyla_circ_noHis355Cm#N/A#N/A
727
726MaxW10-031106C1
25% glycerol in H2O
3E. coliTurbosS356O356pSB1C3-1GateNpuDnaE(N)356Cm#N/A#N/A
728
727MaxW10-031107C1
25% glycerol in H2O
3E. coliTurbosS357O357pSB1C3-1GateNpuDnaE(C)357Cm#N/A#N/A
729
728MaxW10-031108C1
25% glycerol in H2O
3E. coliTurbosS358O358pSB1C3-2Zv2Npu:Xyla(H6)destrCext358Cm#N/A#N/A
730
729MaxW10-031109C1
25% glycerol in H2O
3E. coliTurbosS359O359pSB1C3-T7RBS-FLAG-Smt3-2Zv2Npu359Cm#N/A#N/A
731
730MaxW10-031110C2
25% glycerol in H2O
3E. coliTurbosS360O360pSB1C3-Xylanase360Cm#N/A#N/A
732
731MaxW10-031111C1
25% glycerol in H2O
3E. coliTurbosS361O361pSB1C3-LiLaLy(H6)361Cm#N/A#N/A
733
732MaxW10-031112C1
25% glycerol in H2O
3E. coliTurbosS362O362pSB1C3-XylaH6BB362Cm#N/A#N/A
734
733MaxW10-031113C1
25% glycerol in H2O
3E. coliTurbosS363O363pSB1C3-XylaH6extBB363Cm#N/A#N/A
735
734Lisa10-041135C2
25% glycerol in H2O
3E. coliTurbosO240O371pSBX4C5-1GateSspDnaB(C)371CmDD1467ok
736
735Lisa10-041135C3
25% glycerol in H2O
3E. coliTurbosO240O371-2pSBX4C5-1GateSspDnaB(C)371CmDD1467ok
737
736Lisa10-041136C1
25% glycerol in H2O
3E. coliTurbosO240-3O371-3pSBX4C5-1GateSspDnaB(C)371CmDD1468
ok (maybe there is something in the beginning of mRFP)
738
737Lisa10-041136C2
25% glycerol in H2O
3E. coliTurbosO240-3O371-4pSBX4C5-1GateSspDnaB(C)371CmDD1468
ok (maybe there is something in the beginning of mRFP)
739
738Lisa10-041137C1
25% glycerol in H2O
3E. coliTurbosO241-2O372pSBX4C5-1GateSspDnaB(C*)372CmDD1469ok
740
739Lisa10-041137C2
25% glycerol in H2O
3E. coliTurbosO241-2O372-2pSBX4C5-1GateSspDnaB(C*)372CmDD1469ok
741
740Lisa10-041138C1
25% glycerol in H2O
3E. coliTurbosO241-3O372-3pSBX4C5-1GateSspDnaB(C*)372CmDD1470ok
742
741Lisa10-041138C2
25% glycerol in H2O
3E. coliTurbosO241-3O372-3pSBX4C5-1GateSspDnaB(C*)372CmDD1470ok
743
742Lisa10-041139C2
25% glycerol in H2O
3E. coliTurbosO242O373pSBX4C5-1GateSspDnaB(N)373CmDD1471perfect mRFP read, call GATC
744
743Lisa10-041139C3
25% glycerol in H2O
3E. coliTurbosO242O373-2pSBX4C5-1GateSspDnaB(N)373CmDD1471perfect mRFP read, call GATC
745
744Lisa10-041140C1
25% glycerol in H2O
3E. coliTurbosO242-2O373-3pSBX4C5-1GateSspDnaB(N)373CmDD1472perfect mRFP read, call GATC
746
745Lisa10-041140C2
25% glycerol in H2O
3E. coliTurbosO242-2O373-4pSBX4C5-1GateSspDnaB(N)373CmDD1472perfect mRFP read, call GATC
747
746Lisa10-041141C2
25% glycerol in H2O
3E. coliTurbosO243-2O374pSBX4C5-1GateSspDnaB(N*)374CmDD1473perfect mRFP read, call GATC
748
747Lisa10-041141C3
25% glycerol in H2O
3E. coliTurbosO243-2O374-2pSBX4C5-1GateSspDnaB(N*)374CmDD1473perfect mRFP read, call GATC
749
748Lisa10-041143C1
25% glycerol in H2O
3E. coliTurbosO325O369pSBX4C5-1GateSspDnaE(N)369Cm
DD1475,
perfect mRFP read, call GATC,
750
749Lisa10-041143C2
25% glycerol in H2O
3E. coliTurbosO325O369-2pSBX4C5-1GateSspDnaE(N)369Cm
DD1475,
perfect mRFP read, call GATC,
751
750Lisa10-041144C1
25% glycerol in H2O
3E. coliTurbosO325-2O369-3pSBX4C5-1GateSspDnaE(N)369Cm
DD1476,
perfect mRFP read, call GATC,
752
751Lisa10-041144C3
25% glycerol in H2O
3E. coliTurbosO325-2O369-4pSBX4C5-1GateSspDnaE(N)369Cm
DD1476,
perfect mRFP read, call GATC,
753
752Lisa10-041145C2
25% glycerol in H2O
3E. coliTurbosO326O370pSBX4C5-1GateSspDnaE(C)370CmDD1477double insert insertion
754
753Lisa10-041145C3
25% glycerol in H2O
3E. coliTurbosO326O370-2pSBX4C5-1GateSspDnaE(C)370CmDD1477double insert insertion
755
754Lisa10-041146C2
25% glycerol in H2O
3E. coliTurbosO326-3O370-3pSBX4C5-1GateSspDnaE(C)370CmDD1478tripple insert insertion
756
755Lisa10-041146C3
25% glycerol in H2O
3E. coliTurbosO326-3O370-4pSBX4C5-1GateSspDnaE(C)370CmDD1478tripple insert insertion
757
756MaxW10-041116C1
25% glycerol in H2O
3E. coliTurbosS375O375pSB1C3-T7RBS-H6-sfGFP^N-NpuDnaE^N-H6375Cm#N/A#N/A
758
757MaxW10-041117C1
25% glycerol in H2O
3E. coliTurbosS376O376pSB1C3-1Gate-NpuDnaE(N)-H6376Cm#N/A#N/A
759
758MaxW10-041118C1
25% glycerol in H2O
3E. coliTurbosS377O377pSB1C3-H6-1GateNpuDnaE(C)377Cm#N/A#N/A
760
759MaxW10-041119C1
25% glycerol in H2O
3E. coliTurbosS378O378pSB1C3-T7RBS-H6-sfGFP_T65C-H6378Cm#N/A#N/A
761
760MaxW10-041120C1
25% glycerol in H2O
3E. coliTurbosS379O379pSB1C3-1GateSspDnaX-S11(C)-H6379Cm#N/A#N/A
762
761MaxW10-041121C1
25% glycerol in H2O
3E. coliTurbosS380O380pSB1C3-1GateSspDnaX-S11N_H6380Cm#N/A#N/A
763
762MaxW10-041122C1
25% glycerol in H2O
3E. coliTurbosS381O381pSB1C3-1GateSspGyrB-S11N_H6381Cm#N/A#N/A
764
763MaxW10-041123C1
25% glycerol in H2O
3E. coliTurbosS382O382pSB1C3-1GateTerDnaE3-S11C_H6382Cm#N/A#N/A
765
764MaxW10-041124C1
25% glycerol in H2O
3E. coliTurbosS383O383pSB1C3-1GateTerDnaE3-S11(N)-H6383Cm#N/A#N/A
766
765Caro10-051036C1
25% glycerol in H2O
3E. coliTurbosA271O271-3pSBX1K3-H6-LOV-6-DnaXC-mRFPc-H6271KanDG7164#N/A#N/A
767
766Caro10-051036C2
25% glycerol in H2O
3E. coliTurbosA271O271-4pSBX1K3-H6-LOV-6-DnaXC-mRFPc-H6271KanDG7165#N/A#N/A
768
767Caro10-051036C3
25% glycerol in H2O
3E. coliTurbosA271O271-5pSBX1K3-H6-LOV-6-DnaXC-mRFPc-H6271Kan#N/A#N/A
769
768Caro10-051095C1
25% glycerol in H2O
3E. coliTurbosA272#3O272-4pSBX1K3-H6-DnaXC-mRFPc-H6272KanDG7162#N/A#N/A
770
769Caro10-051095C2
25% glycerol in H2O
3E. coliTurbosA272#3O272-5pSBX1K3-H6-DnaXC-mRFPc-H6272KanDG7163#N/A#N/A
771
770Caro10-051095C3
25% glycerol in H2O
3E. coliTurbosA272#3O272-6pSBX1K3-H6-DnaXC-mRFPc-H6272Kan#N/A#N/A
772
771Caro10-051096C1
25% glycerol in H2O
3E. coliTurbosA275#3O275-4pSBX1K3-SspDnaXc*-sfGFP^C-H6275KanDG7166#N/A#N/A
773
772Caro10-051096C2
25% glycerol in H2O
3E. coliTurbosA275#3O275-5pSBX1K3-SspDnaXc*-sfGFP^C-H6275KanDG7167#N/A#N/A
774
773Caro10-051096C4
25% glycerol in H2O
3E. coliTurbosA275#3O275-7pSBX1K3-SspDnaXc*-sfGFP^C-H6275Kan#N/A#N/A
775
774Caro10-051125C1
25% glycerol in H2O
3E. coliTurbosG312#2O312-4pSBX4C5-0GateTerDnaE3-S11C*_mRFP*169-225312CmDG7168#N/A#N/A
776
775Caro10-051125C2
25% glycerol in H2O
3E. coliTurbosG312#2O312-5pSBX4C5-0GateTerDnaE3-S11C*_mRFP*169-225312Cm#N/A#N/A
777
776Caro10-051125C3
25% glycerol in H2O
3E. coliTurbosG312#2O312-6pSBX4C5-0GateTerDnaE3-S11C*_mRFP*169-225312Cm#N/A#N/A
778
777Caro10-051126C1
25% glycerol in H2O
3E. coliTurbosG313#2O313-4pSBX4C5-0GateTerDnaE3-S11N*_mRFP*1-168313CmDG7169ok, good#N/A#N/A
779
778Caro10-051126C2
25% glycerol in H2O
3E. coliTurbosG313#2O313-5pSBX4C5-0GateTerDnaE3-S11N*_mRFP*1-168313Cm#N/A#N/A
780
779Caro10-051126C3
25% glycerol in H2O
3E. coliTurbosG313#2O313-6pSBX4C5-0GateTerDnaE3-S11N*_mRFP*1-168313Cm#N/A#N/A
781
780Caro10-051127C1
25% glycerol in H2O
3E. coliTurbosG314#2O314-4pSBX4C5-0GateNpuDnaE_C*_mRFP*169-225314CmDG7170#N/A#N/A
782
781Caro10-051127C2
25% glycerol in H2O
3E. coliTurbosG314#2O314-5pSBX4C5-0GateNpuDnaE_C*_mRFP*169-225314CmDG7171#N/A#N/A
783
782Caro10-051127C3
25% glycerol in H2O
3E. coliTurbosG314#2O314-6pSBX4C5-0GateNpuDnaE_C*_mRFP*169-225314Cm#N/A#N/A
784
783Caro10-051128C1
25% glycerol in H2O
3E. coliTurbosG315#2O315-4pSBX4C5-0GateNpuDnaE_N*_mRFP*1-168315CmDG7172ok, good#N/A#N/A
785
784Caro10-051128C2
25% glycerol in H2O
3E. coliTurbosG315#2O315-5pSBX4C5-0GateNpuDnaE_N*_mRFP*1-168315CmDG7173
ok, but probably mutation in promoter, if possible use O315-4
#N/A#N/A
786
785Caro10-051128C3
25% glycerol in H2O
3E. coliTurbosG315#2O315-6pSBX4C5-0GateNpuDnaE_N*_mRFP*1-168315Cm#N/A#N/A
787
786Jan10-051149C1
25% glycerol in H2O
3E. coliTurbosS398O398pSB1C3-1GateNpuDnaE(N)*-H6398Cm#N/A#N/A
788
787Jan10-051150C1
25% glycerol in H2O
3E. coliTurbosS399O399pSB1C3-1GateSspGyrB-S11C_H6399Cm#N/A#N/A
789
788Jan10-051151C1
25% glycerol in H2O
3E. coliTurbosS400O400pSB1C3-H6-1GateNpu*(C)_v2400Cm#N/A#N/A
790
789Jan10-051152C1
25% glycerol in H2O
3E. coliTurbosS401O401pSB1C3-1Gate-NpuDnaE(N)-H6-H6-1GateNpuDnaE(C)401Cm#N/A#N/A
791
790Jan10-051153C1
25% glycerol in H2O
3E. coliTurbosS402O402pSB1C3-1GateNpuDnaE(N)*-H6-H6-1GateNpu*(C)_v2402Cm#N/A#N/A
792
791Jan10-051154C1
25% glycerol in H2O
3E. coliTurbosS403O403pSB1C3-T7RBS_BBv2403Cm#N/A#N/A
793
792Jan10-051154C2
25% glycerol in H2O
3E. coliTurbosS403O403-2pSB1C3-T7RBS_BBv2403Cm#N/A#N/A
794
793Jan10-051155C1
25% glycerol in H2O
3E. coliTurbosS284O284-3pSBX1K3-2Zv2Npu:LaLys+bad1284Kan#N/A#N/A
795
794MaxW10-061179C1
25% glycerol in H2O
3E. coliTurbosS417O417pSB1C3-1GateGp41-1(N)-H6417Cm#N/A#N/A
796
795MaxW10-061181C1
25% glycerol in H2O
3E. coliTurbosS418O418pSB1C3-H6-1GateGp41-1(C)418Cm#N/A#N/A
797
796MaxW10-061183C1
25% glycerol in H2O
3E. coliTurbosS419O419pSB1C3-DoubleGateGp41-1(N)419Cm#N/A#N/A
798
797MaxW10-061185C1
25% glycerol in H2O
3E. coliTurbosS420O420pSB1C3-DoubleGateGp41-1(C)420Cm#N/A#N/A
799
798MaxW10-061187C1
25% glycerol in H2O
3E. coliTurbosS421O421pSB1C3-DoubleNpuDnaE(N)421Cm#N/A#N/A
800
799MaxW10-061189C1
25% glycerol in H2O
3E. coliTurbosS422O422pSB1C3-DoubleGateNpuDnaE(C)422Cm#N/A#N/A
801
800MaxW10-061190C1
25% glycerol in H2O
3E. coliTurbosS404O404pSBX1A3-BBa_J04450404Amp#N/A#N/A
802
801MaxW10-061191C1
25% glycerol in H2O
3E. coliTurbosS405O405pSBX1C3-BBa_J04450405Cm#N/A#N/A
803
802MaxW10-061192C1
25% glycerol in H2O
3E. coliTurbosS406O406pSBX1K3-BBa_J04450406Kan#N/A#N/A
804
803MaxW10-061194C1
25% glycerol in H2O
3E. coliTurbosS408O408pSBX4A5-BBa_J04450408Amp#N/A#N/A
805
804MaxW10-061195C1
25% glycerol in H2O
3E. coliTurbosS409O409pSBX4C5-BBa_J04450409Cm#N/A#N/A
806
805Silvan10-07954C1-3
25% glycerol in H2O
7E. coli
Rosetta (DE3)
O232pSBX1K3-T7RBS-FLAG-Smt3-2ZNpu:(CWE-mDNMT1(731-1602)-H6SGRGT), pRARE23231Kan, Cm#N/A
DD1384, DD1391
ok, ok
807
806Silvan10-07955C1-2
25% glycerol in H2O
6E. coliBI21(DE3)O239-3pSBX1K3-T7RBS-2ZSortA:eGFP-H6239Kan#N/A
DD1387, DD139428HE01
no result, no resultok
808
807Silvan10-07978C1-3
25% glycerol in H2O
7E. coli
Rosetta (DE3)
O250pSBX1K3-T7RBS-FLAG-2ZNpu:(CWE-mDNMT1(731-1602)-0917/2r-H6SGRGT), pRARE25031Kan, Cm#N/A28HD94ok
809
808Silvan10-07980C1-2
25% glycerol in H2O
7E. coli
Rosetta (DE3)
O253pSBX1K3-T7RBS-Smt3-2ZSortA:(G4-mDNMT1(731-1602)-0915/4r-FLAG)-H6, pRARE25331Kan, Cm#N/A28HD83ok
810
809Silvan10-071001C1-3
25% glycerol in H2O
7E. coli
Rosetta (DE3)
O254-2pSBX1K3-T7RBS-FLAG-2ZNpu:(CWE-mDNMT1(731-1602)-0915/3f-H6SGRGT), pRARE25431Kan, Cm#N/A28HD93ok
811
810Silvan10-071002C1-2
25% glycerol in H2O
7E. coli
Rosetta (DE3)
O265-2pSBX1K3-T7RBS-Smt3-2ZSortA:(G2-mDNMT1(731-1602)-0917/1r)-H6, pRARE26531Kan, Cm#N/A28HD92ok
812
811Lisa10-071156C2
25% glycerol in H2O
3E. coliTurbosS414O414pSB1C3-1GateSspDnaB(C*)414Cm
DG7142, IC5216, IC5221
bad, SspDnaEN, SspDnaEN#N/A#N/A
813
812Lisa10-071157C1
25% glycerol in H2O
3E. coliTurbosS414#2O414-2pSB1C3-1GateSspDnaB(C*)414CmDG7143#N/A#N/A
814
813Lisa10-071158C1
25% glycerol in H2O
3E. coliTurbosS414#3O414-3pSB1C3-1GateSspDnaB(C*)414CmDG7144#N/A#N/A
815
814Lisa10-071159C2
25% glycerol in H2O
3E. coliTurbosS414#4O414-4pSB1C3-1GateSspDnaB(C*)414CmDG7145#N/A#N/A
816
815Lisa10-071160C2
25% glycerol in H2O
3E. coliTurbosS415O415pSB1C3-1GateSspDnaB(N)415Cm
DG7146, IC5213, IC5218
bad, actually SspDnaEC, actually SspDnaEC#N/A#N/A
817
816Lisa10-071161C1
25% glycerol in H2O
3E. coliTurbosS415#2O415-2pSB1C3-1GateSspDnaB(N)415CmDG7147bad#N/A#N/A
818
817Lisa10-071162C1
25% glycerol in H2O
3E. coliTurbosS415#3O415-3pSB1C3-1GateSspDnaB(N)415CmDG7148bad#N/A#N/A
819
818Lisa10-071163C2
25% glycerol in H2O
3E. coliTurbosS415#4O415-4pSB1C3-1GateSspDnaB(N)415CmDG7149bad#N/A#N/A
820
819Lisa10-071164C1
25% glycerol in H2O
3E. coliTurbosS416O416pSB1C3-1GateSspDnaB(N*)416Cm
DG7150, IC5215, IC5220
bad, actually SspDnaBC!!!!!! wuhuuuuuuuuuu yippie, actually SspDnaBC!!!!!! wuhuuuuuuuuuu yippie
#N/A#N/A
821
820Lisa10-071166C2
25% glycerol in H2O
3E. coliTurbosS411O411pSB1C3-1GateSspDnaE(N)411CmDG7152#N/A#N/A
822
821Lisa10-071167C1
25% glycerol in H2O
3E. coliTurbosS411#2O411-2pSB1C3-1GateSspDnaE(N)411CmDG7153#N/A#N/A
823
822Lisa10-071168C1
25% glycerol in H2O
3E. coliTurbosS411#3O411-3pSB1C3-1GateSspDnaE(N)411CmDG7154#N/A#N/A
824
823Lisa10-071169C1
25% glycerol in H2O
3E. coliTurbosS411#4O411-4pSB1C3-1GateSspDnaE(N)411Cm
DG7155, IC5217, IC5222
, actually SspDnaBC*, actually SspDnaBC*#N/A#N/A
825
824Lisa10-071165C2
25% glycerol in H2O
3E. coliTurbosS416#2O416-2pSB1C3-1GateSspDnaB(N*)416CmDG7151not mixed up, missing?#N/A#N/A
826
825Lisa10-071170C2
25% glycerol in H2O
3E. coliTurbosS412O412pSB1C3-1GateSspDnaE(C)412CmDG7156#N/A#N/A
827
826Lisa10-071171C1
25% glycerol in H2O
3E. coliTurbosS412#2O412-2pSB1C3-1GateSspDnaE(C)412Cm
DG7157, IC5260
, actually sspdnaBN#N/A#N/A
828
827Lisa10-071172C1
25% glycerol in H2O
3E. coliTurbosS412#3O412-3pSB1C3-1GateSspDnaE(C)412Cm
DG7158, IC5261
, actually sspdnaBN#N/A#N/A
829
828Lisa10-071173C2
25% glycerol in H2O
3E. coliTurbosS412#4O412-4pSB1C3-1GateSspDnaE(C)412CmDG7159#N/A#N/A
830
829Lisa10-071175C1
25% glycerol in H2O
3E. coliTurbosS413#2O413pSB1C3-1GateSspDnaB(C)413CmDG7160#N/A#N/A
831
830Lisa10-071175C2
25% glycerol in H2O
3E. coliTurbosS413#2O413-2pSB1C3-1GateSspDnaB(C)413Cm
DG7161, IC5214, IC5219
bad, actually SspDnaBN*, actually SspDnaBN*
#N/A#N/A
832
831Caro10-071205C1
25% glycerol in H2O
3E. coliTurbosS386#3O386pSBX4C5-0GateTer*-coex_mRFP168/169386Cm
IC5185, IC5186
good, ok, good, ok#N/A#N/A
833
832Caro10-071207C1
25% glycerol in H2O
3E. coliTurbosS387#2O387pSBX4C5-0GateNpu*-coex_mRFP168/169387Cm
IC5187, IC5188
good, ok, good, ok#N/A#N/A
834
833Caro10-071208C1
25% glycerol in H2O
3E. coliTurbosS387#3O387-2pSBX4C5-0GateNpu*-coex_mRFP168/169387Cm#N/A#N/A
835
834Caro10-071201C2
25% glycerol in H2O
3E. coliTurbosS384O384pSBX4C5-0GateDnaX*-coex_mRFP168/169384Cm
IC5189, IC5190
no DnaXN, no DNnaXN#N/A#N/A
836
835Caro10-071203C1
25% glycerol in H2O
3E. coliTurbosS386O386-2pSBX4C5-0GateTer*-coex_mRFP168/169386Cm#N/A#N/A
837
836Caro10-071204C2
25% glycerol in H2O
3E. coliTurbosS386#2O386-3pSBX4C5-0GateTer*-coex_mRFP168/169386Cm#N/A#N/A
838
837Caro10-071209C1
25% glycerol in H2O
3E. coliTurbosS397O397pSBX1K3-DnaX-mRFP168/169_coex397Kan
IC5191, IC5192
good, ok, good, ok#N/A#N/A
839
838Caro10-071198C1
25% glycerol in H2O
3E. coliTurbosS255#3O255-3pSBX4C5-0GateSspDnaX-coex_mRFP168/169255Cm
IC5193, IC5194
good, ok, good, ok#N/A#N/A
840
839Caro10-071198C3
25% glycerol in H2O
3E. coliTurbosS255#3O255-4pSBX4C5-0GateSspDnaX-coex_mRFP168/169255Cm#N/A#N/A
841
840Caro10-071199C1
25% glycerol in H2O
3E. coliTurbosS256#2O256pSBX4C5-0GateSspGyrB-coex_mRFP168/169256Cm
IC5195, IC5196
good, ok, good, ok#N/A#N/A
842
841Caro10-071200C1
25% glycerol in H2O
3E. coliTurbosS257#3O257-3pSBX4C5-0GateTerDnaE3-coex_mRFP168/169257Cm
IC5197, IC5198
mutations after Biobrick, good ok, mutations after Biobrick, good ok
#N/A#N/A
843
842Caro10-071213C2
25% glycerol in H2O
3E. coliTurbosS393O393pSBX1K3-H6-LOV-BsaIIasB-mRFP_coex393Kan
IC5199, IC5200
ok, good, ok, good#N/A#N/A
844
843Caro10-071212C1
25% glycerol in H2O
3E. coliTurbosS396O396pSBX1K3-DnaX-mRFP*168/169_coex396Kan
IC5201, IC5202
ok, good, ok, good#N/A#N/A
845
844Caro10-071212C2
25% glycerol in H2O
3E. coliTurbosS396O396-2pSBX1K3-DnaX-mRFP*168/169_coex396Kan#N/A#N/A
846
845Caro10-071211C1
25% glycerol in H2O
3E. coliTurbosS397#3O397-2pSBX1K3-DnaX-mRFP168/169_coex397Kan#N/A#N/A
847
846Caro10-071210C2
25% glycerol in H2O
3E. coliTurbosS397#2O397-3pSBX1K3-DnaX-mRFP168/169_coex397Kan#N/A#N/A
848
847Caro10-071215C1
25% glycerol in H2O
3E. coliTurbosS395O395pSBX1K3-H6-LOV-6-DnaXC-mRFP_coex395Kan
IC5203, IC5204
ok, good, probably frameshift after CDS, ok, good, probably frameshift after CDS
#N/A#N/A
849
848Caro10-071216C1
25% glycerol in H2O
3E. coliTurbosS395#2O395-2pSBX1K3-H6-LOV-6-DnaXC-mRFP_coex395Kan#N/A#N/A
850
849Caro10-071217C1
25% glycerol in H2O
3E. coliTurbosS395#3O395-3pSBX1K3-H6-LOV-6-DnaXC-mRFP_coex395Kan#N/A#N/A
851
850Caro10-071218C1
25% glycerol in H2O
3E. coliTurbosS391O391pSBX1K3-DnaX-sfGFP_coex391Kan
IC5205, IC5206
ok, good, ok,good#N/A#N/A
852
851Caro10-071219C1
25% glycerol in H2O
3E. coliTurbosS392O392pSBX1K3-DnaX*-sfGFP_coex392Kan
IC5207, IC5208
ok, good, ok,good#N/A#N/A
853
852Caro10-071220C3
25% glycerol in H2O
3E. coliTurbosS392#2O392-2pSBX1K3-DnaX*-sfGFP_coex392Kan#N/A#N/A
854
853Caro10-071221C1
25% glycerol in H2O
3E. coliTurbosS392#3O392-3pSBX1K3-DnaX*-sfGFP_coex392Kan#N/A#N/A
855
854Caro10-071222C1
25% glycerol in H2O
3E. coliTurbosS388O388pSBX1K3-LOV-BsaIIasB-sfGFP_coex388Kan
IC5209, IC5210
ok, good, ok,good#N/A#N/A
856
855Caro10-071223C1
25% glycerol in H2O
3E. coliTurbosS389O389pSBX1K3-LOV-12-DnaXC-sfGFP_coex389Kan
IC5211, IC5212
ok, good, ok,good#N/A#N/A
857
856Silvan10-0766C1-17
25% glycerol in H2O
7E. coli
Rosetta (DE3)
pRARE31Cm#N/A
858
857Silvan10-071226C1
25% glycerol in H2O
3E. coliTurbosG235pSB1C3-Smt3-2ZSortA-H6 (mRFP)235Cm#N/A#N/A#N/A
859
858Silvan10-071226C2
25% glycerol in H2O
3E. coliTurbosG235O235pSB1C3-Smt3-2ZSortA-H6 (mRFP)235Cm
IC5225, IC5231
C->A point mutation in mRFP barcode, mixture of mRFP and ccdB
#N/A#N/A
860
859Silvan10-071226C3
25% glycerol in H2O
3E. coliTurbosG235O235-2pSB1C3-Smt3-2ZSortA-H6 (mRFP)235CmIC5226, C->A point mutation in mRFP barcode, #N/A#N/A
861
860Silvan10-071228C1
25% glycerol in H2O
3E. coliTurbosS234pSB1C3-T7RBS-Smt3-2ZSortA-H6 (mRFP)234Cm#N/A#N/A#N/A
862
861Silvan10-071228C2
25% glycerol in H2O
3E. coliTurbosS234O234pSB1C3-T7RBS-Smt3-2ZSortA-H6 (mRFP)234Cm#N/A#N/A
863
862Silvan10-071228C3
25% glycerol in H2O
3E. coliTurbosS234O234-2pSB1C3-T7RBS-Smt3-2ZSortA-H6 (mRFP)234Cm#N/A#N/A
864
863Silvan10-071229C1
25% glycerol in H2O
3E. coliTurbosS423O423pSB1C3-T7RBS-H6-Smt3-0ZSortA423Cm#N/A#N/A
865
864Silvan10-071229C2
25% glycerol in H2O
3E. coliTurbosS423O423-2pSB1C3-T7RBS-H6-Smt3-0ZSortA423Cm#N/A#N/A
866
865Silvan10-071229C3
25% glycerol in H2O
3E. coliTurbosS423O423-3pSB1C3-T7RBS-H6-Smt3-0ZSortA423Cm#N/A#N/A
867
866Silvan10-071230C1
25% glycerol in H2O
3E. coliTurbosS425O425pSB1C3-T7RBS-H6-0ZSortA425Cm#N/A#N/A
868
867Silvan10-071230C2
25% glycerol in H2O
3E. coliTurbosS425O425-2pSB1C3-T7RBS-H6-0ZSortA425Cm#N/A#N/A
869
868Silvan10-071230C3
25% glycerol in H2O
3E. coliTurbosS425O425-3pSB1C3-T7RBS-H6-0ZSortA425Cm#N/A#N/A
870
869Silvan10-071231C1
25% glycerol in H2O
3E. coliTurbosA177#4O177-4pSB1C3-2ZSortA-H6177Cm
IC5227, IC5232
ok, ok#N/A#N/A
871
870Silvan10-071231C2
25% glycerol in H2O
3E. coliTurbosA177#4O177-5pSB1C3-2ZSortA-H6177Cm
IC5228, IC5233
ok, ok#N/A#N/A
872
871Silvan10-071231C3
25% glycerol in H2O
3E. coliTurbosA177#4O177-6pSB1C3-2ZSortA-H6177Cm#N/A#N/A
873
872Silvan10-071232C1
25% glycerol in H2O
3E. coliTurbosA426O426pSB1C3-H6-Smt3-mDNMT1(731-1602)426Cm
IC5229, IC5234, IC5236, DD1482
is actually pSB1C3-2ZSortA-H6 with silent G->T mutation in mRFP, is actually pSB1C3-2ZSortA-H6 with silent G->T mutation in mRFP, no result, is actually pSB1C3-2ZSortA-H6 with silent G->T mutation in mRFP
#N/A#N/A
874
873Silvan10-071232C2
25% glycerol in H2O
3E. coliTurbosA426O426-2pSB1C3-H6-Smt3-mDNMT1(731-1602)426Cm
IC5230, IC5235, DD1481, DD1483
ok, ok, ok, ok#N/A#N/A
875
874Silvan10-071232C3
25% glycerol in H2O
3E. coliTurbosA426O426-3pSB1C3-H6-Smt3-mDNMT1(731-1602)426Cm#N/A#N/A
876
875Caro10-101235C1
25% glycerol in H2O
6E. coliBI21(DE3)O389pSBX1K3-LOV-12-DnaXC-sfGFP_coex389Kan#N/A
IC5211, IC5212
ok, good, ok,good
877
876Caro10-101236C1
25% glycerol in H2O
6E. coliBI21(DE3)O392pSBX1K3-DnaX*-sfGFP_coex392Kan#N/A
IC5207, IC5208
ok, good, ok,good
878
877Caro10-101237C1
25% glycerol in H2O
6E. coliBI21(DE3)O391pSBX1K3-DnaX-sfGFP_coex391Kan#N/A
IC5205, IC5206
ok, good, ok,good
879
878Caro10-101238C1
25% glycerol in H2O
6E. coliBI21(DE3)O278pSBX1K3-DnaXN-sfGFPn-H6278Kan#N/ADD1413ok one additional His
880
879Caro10-101239C1
25% glycerol in H2O
6E. coliBI21(DE3)O276pSBX1K3-SspDnaXc-sfGFP^C-H6276Kan#N/ADD1417ok? two additional His
881
880Caro10-101240C1
25% glycerol in H2O
6E. coliBI21(DE3)O395pSBX1K3-H6-LOV-6-DnaXC-mRFP_coex395Kan#N/A
IC5203, IC5204
ok, good, probably frameshift after CDS, ok, good, probably frameshift after CDS
882
881Caro10-101241C1
25% glycerol in H2O
6E. coliBI21(DE3)O396pSBX1K3-DnaX-mRFP*168/169_coex396Kan#N/A
IC5201, IC5202
ok, good, ok, good
883
882Caro10-101242C1
25% glycerol in H2O
6E. coliBI21(DE3)O397pSBX1K3-DnaX-mRFP168/169_coex397Kan#N/A
IC5191, IC5192
good, ok, good, ok
884
883Caro10-101243C1
25% glycerol in H2O
6E. coliBI21(DE3)O272-4pSBX1K3-H6-DnaXC-mRFPc-H6272Kan#N/ADG7162
885
884Caro10-101244C1
25% glycerol in H2O
6E. coliBI21(DE3)O267-3pSBX1K3-H6-mRFPn-DnaXN-H6267Kan#N/A28HE15ok, good
886
885Caro10-101245C1
25% glycerol in H2O
6E. coliBI21(DE3)O260-5O260-6pSBX1K3-BBa_I13507-GSGIDECHT260Kan
DD2690, DD2691
,
887
886Caro10-101245C2
25% glycerol in H2O
6E. coliBI21(DE3)O260-5O260-7pSBX1K3-BBa_I13507-GSGIDECHT260Kan
DD2692, DD2693
,
888
887Caro10-101245C3
25% glycerol in H2O
6E. coliBI21(DE3)O260-5pSBX1K3-BBa_I13507-GSGIDECHT260Kan#N/A
889
888Caro10-101247C1
25% glycerol in H2O
6E. coliBI21(DE3)O59-14pSBX1K3-BBa_I1350759Kan#N/A
890
889Caro10-101248C1
25% glycerol in H2O
7E. coli
Rosetta (DE3)
O271-327131,29#N/A#N/ADG7164
891
890Caro10-101246C1
25% glycerol in H2O
6E. coliBI21(DE3)O261-3O261-4pSBX1K3-BBa_I13507-GSGIDECHT-Jalpha261Kan
DD2694, DD2695
,
892
891Caro10-101246C2
25% glycerol in H2O
6E. coliBI21(DE3)O261-3O261-5pSBX1K3-BBa_I13507-GSGIDECHT-Jalpha261Kan
DD2696, DD2697
,
893
892Caro10-101256C1
25% glycerol in H2O
3E. coliTurbosO261-3O261-6pSBX1K3-BBa_I13507-GSGIDECHT-Jalpha261Kan
DD2698, DD2699
,
894
89310-131257C1
25% glycerol in H2O
#N/A#N/A#N/AO394#N/A#N/A#N/A#N/A#N/A
DD2689, DD2688
ok, good, ok, good
895
894
25% glycerol in H2O
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
896
895
25% glycerol in H2O
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
897
896
25% glycerol in H2O
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
898
897
25% glycerol in H2O
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
899
898
25% glycerol in H2O
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
900
899
25% glycerol in H2O
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
901
900
25% glycerol in H2O
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
902
901
25% glycerol in H2O
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
903
902
25% glycerol in H2O
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
904
903
25% glycerol in H2O
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
905
904
25% glycerol in H2O
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
906
905
25% glycerol in H2O
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
907
906
25% glycerol in H2O
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
908
907
25% glycerol in H2O
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
909
908
25% glycerol in H2O
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
910
909
25% glycerol in H2O
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
911
910
25% glycerol in H2O
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
912
911
25% glycerol in H2O
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
913
912
25% glycerol in H2O
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
914
913
25% glycerol in H2O
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
915
914
25% glycerol in H2O
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
916
915
25% glycerol in H2O
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
917
916
25% glycerol in H2O
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
918
917
25% glycerol in H2O
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
919
918
25% glycerol in H2O
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
920
919
25% glycerol in H2O
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
921
920
25% glycerol in H2O
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
922
921
25% glycerol in H2O
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
923
922
25% glycerol in H2O
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
924
923
25% glycerol in H2O
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
925
924
25% glycerol in H2O
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
926
925
25% glycerol in H2O
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
927
926
25% glycerol in H2O
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
928
927
25% glycerol in H2O
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
929
928
25% glycerol in H2O
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
930
929
25% glycerol in H2O
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
931
930
25% glycerol in H2O
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
932
931
25% glycerol in H2O
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
933
932
25% glycerol in H2O
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
934
933
25% glycerol in H2O
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
935
934
25% glycerol in H2O
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
936
935
25% glycerol in H2O
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
937
936
25% glycerol in H2O
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
938
937
25% glycerol in H2O
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
939
938
25% glycerol in H2O
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
940
939
25% glycerol in H2O
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
941
940
25% glycerol in H2O
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
942
941
25% glycerol in H2O
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
943
942
25% glycerol in H2O
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
944
943
25% glycerol in H2O
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
945
944
25% glycerol in H2O
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
946
945
25% glycerol in H2O
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
947
946
25% glycerol in H2O
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
948
947
25% glycerol in H2O
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
949
948
25% glycerol in H2O
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
950
949
25% glycerol in H2O
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
951
950
25% glycerol in H2O
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
952
951
25% glycerol in H2O
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
953
952
25% glycerol in H2O
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
954
953
25% glycerol in H2O
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
955
954
25% glycerol in H2O
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
956
955
25% glycerol in H2O
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
957
956
25% glycerol in H2O
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
958
957
25% glycerol in H2O
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
959
958
25% glycerol in H2O
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
960
959
25% glycerol in H2O
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
961
960
25% glycerol in H2O
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
962
961
25% glycerol in H2O
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
963
962
25% glycerol in H2O
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
964
963
25% glycerol in H2O
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
965
964
25% glycerol in H2O
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
966
965
25% glycerol in H2O
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
967
966
25% glycerol in H2O
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
968
967
25% glycerol in H2O
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
969
968
25% glycerol in H2O
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
970
969
25% glycerol in H2O
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
971
970
25% glycerol in H2O
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
972
971
25% glycerol in H2O
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
973
972
25% glycerol in H2O
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
974
973
25% glycerol in H2O
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
975
974
25% glycerol in H2O
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
976
975
25% glycerol in H2O
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
977
976
25% glycerol in H2O
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
978
977
25% glycerol in H2O
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
979
978
25% glycerol in H2O
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
980
979
25% glycerol in H2O
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
981
980
25% glycerol in H2O
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
982
981
25% glycerol in H2O
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
983
982
25% glycerol in H2O
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
984
983
25% glycerol in H2O
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
< insert growth id
< insert growth id
985
984
25% glycerol in H2O
< insert growth id
#N/A#N/A
< insert growth id
< insert growth id
< insert growth id
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986
985
25% glycerol in H2O
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#N/A#N/A
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