Team:Heidelberg/AwesomeSheet/Blots

From 2014.igem.org

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– Blots

	A	B	C	D	E	F	G	H	I	J	K	L	M	N	O	P	Q	R	S	T	U	V	W	X	Y	Z	AA	AB	AC	AD	AE	AF	AG	AH	AI
1			Transfer												BIocking
2	ID	Gel ID	Membrane	Transfer-Buffer	U	I/A	t	H20	Ponceau-Stain	H20	Picture	Wash					Sovent		Wash			Primary				Wash			Secondary				Wash
3					V	mA/cm^2	min	t	t	t		#	t		c	Name		t	#	Reagent	t	Antibody	Solvent	V	c	#	Reagent	t	Antibody	Solvent	V	c	#	Reagent	t
4								min	min	min			min		% (m/V)			min			min			ml				min			ml				min
5					20		60	10	10	1			10

6	1
7	2
8	3
9	4
10	5
11	6	7	PVFD		20		60	60	30	1			40		5	BSA	PBS-T	720	3	TBS-T	10	mouse anti-H5				3	TBS-T	10					4	TBS-T	10
12	7	22	PVFD		20	4	60
13	8	23	PVFD	Tris-Glycin+20% MetOH	25	4	60	10	10	40			20		3	BSA	TBS	60	3	TBS-T/TBS-T/TBS	10	mouse anti-H5	3% BSA in TBS	5	1/1/1000	3	TBS-T/TBS-T/TBS	10	goat? anti mouse	3% BSA in TBS	5	12:20	4	TBS-T/TBS-T/TBS-/&TBS	10
14	9	32			25	4	50	20	2	10		3		TBS-T/TBS/TBS	3	BSA	TBS
15	10	33
16	11	34
17	12	31
18	13	31
19	14	31
20	15	31
21	16	49	PVFD	Tris-Glycin+20% MetOH		0.04/1/4	420	10	5	10		2	10		3	BSA	TBS	60	3	TBS-T	10	mouse anti-H5	3% BSA in TBS			3	TBS-T		goat anti-mouse					TBS-T
22	17	50	PVFD	Tris-Glycin+20% MetOH		0.04/1/4	420	10	5	10		2	10		3	BSA	TBS	60	3	TBS-T	10	mouse anti-H5	3% BSA in TBS			3	TBS-T		goat anti-mouse					TBS-T
23	18	51	PVFD	Tris-Glycin+20% MetOH		0.04/1/4	420	10	5	10		2	10		3	BSA	TBS	60	3	TBS-T	10	mouse anti-H5	3% BSA in TBS			3	TBS-T		goat anti-mouse					TBS-T
24	19	52	PVFD	Tris-Glycin+20% MetOH		4	420	10	2	10		2	10		3	BSA	TBS	60	3	TBS-T	10	mouse anti-H5	3% BSA in TBS			3	TBS-T		goat anti-mouse					TBS-T
25	20	53	PVFD	Tris-Glycin+20% MetOH		4	420	10	2	10		2	10		3	BSA	TBS	60	3	TBS-T	10	mouse anti-H5	3% BSA in TBS			3	TBS-T		goat anti-mouse					TBS-T
26	21	54	PVFD	Tris-Glycin+20% MetOH		4	420	10	2	10		2	10		3	BSA	TBS	60	3	TBS-T	10	mouse anti-H5	3% BSA in TBS			3	TBS-T		goat anti-mouse					TBS-T
27	22	58	PVFD	Tris-Glycin+20% MetOH	25	4	60	0	2	10		2	10		3	BSA	TBS	60		TBS-T	10	mouse anti-H5					TBS-T		goat anti-mouse
28	23
29	24
30	25
31	26
32	27
33	28
34	29
35	30
36	31
37	32
38	33
39	34
40	35
41	36
42	37
43	38
44	39
45	40
46	41
47	42
48	43
49	44
50	45
51	46
52	47
53	48
54	49
55	50
56	51
57	52
58	53
59	54
60	55
61	56
62	57
63	58
64	59
65	60
66	61
67	62
68	63
69	64
70	65
71	66
72	67
73	68
74	69
75	70
76	71
77	72
78	73
79	74
80	75
81	76
82	77
83	78
84	79
85	80
86	81
87	82
88	83
89	84
90	85

Team:Heidelberg/AwesomeSheet/Blots

From 2014.igem.org

– Blots

Awesome Sheet PA Gels Dnmt1 Methylation Assay Blots Waste SURPREMErun Strains Stocks Samples Purifications Plate Reader Marker LIGHTRun Enzymes Competent cells Induction Growth 3AL AGels AssmProd Assembly GoldenGate Parts Submission Parts PCR products PCRs Plasmids Plates Primer Res SL Trafos