first test of template + restrictione enzymes without Dnmt1
none
TAE 20%
none
80
60
blurry bands
4
1
8/9/2014
allme
0.2
none
0.2
10
TAE 20%
none
80
60
5
1
8/9/2014
1CRFme (semi)
0.2
none
0.2
10
TAE 20%
none
80
60
6
1
8/9/2014
1CRme
0.2
none
0.2
10
TAE 20%
none
80
60
7
1
8/9/2014
unme
0.2
none
0.2
10
TAE 20%
none
80
60
8
1
8/9/2014
allme
0.2
Sau3AI
0.2
1h
1.1
1
10
TAE 20%
none
80
60
9
1
8/9/2014
1CRFme (semi)
0.2
Sau3AI
0.2
1h
1.1
1
10
TAE 20%
none
80
60
10
1
8/9/2014
1CRme
0.2
Sau3AI
0.2
1h
1.1
1
10
TAE 20%
none
80
60
11
1
8/9/2014
unme
0.2
Sau3AI
0.2
1h
1.1
1
10
TAE 20%
none
80
60
12
1
8/9/2014
allme
0.2
HpaIII
0.2
15 min
CutSmart
1
10
TAE 20%
none
80
60
13
1
8/9/2014
1CRFme (semi)
0.2
HpaIII
0.2
15 min
CutSmart
1
10
TAE 20%
none
80
60
14
1
8/9/2014
1CRme
0.2
HpaIII
0.2
15 min
CutSmart
1
10
TAE 20%
none
80
60
15
1
8/9/2014
unme
0.2
HpaIII
0.2
15 min
CutSmart
1
10
TAE 20%
none
80
60
16
2
M
8/10/2014
TAE 20%
even more blurry bands
17
2
8/10/2014
allme
0.4
none
0.2
10
TAE 20%
none
80
90
18
2
8/10/2014
1CRFme (semi)
0.4
none
0.2
10
TAE 20%
none
80
90
19
2
8/10/2014
1CRme
0.4
none
0.2
10
TAE 20%
none
80
90
20
2
8/10/2014
unme
0.4
none
0.2
10
TAE 20%
none
80
90
21
2
8/10/2014
allme
0.4
Sau3AI
0.2
1h
1.1
1
10
TAE 20%
none
80
90
22
2
8/10/2014
1CRFme (semi)
0.4
Sau3AI
0.2
1h
1.1
1
10
TAE 20%
none
80
90
23
2
8/10/2014
1CRme
0.4
Sau3AI
0.2
1h
1.1
1
10
TAE 20%
none
80
90
24
2
8/10/2014
unme
0.4
Sau3AI
0.2
1h
1.1
1
10
TAE 20%
none
80
90
25
2
8/10/2014
allme
0.4
HpaIII
0.2
15 min
CutSmart
1
10
TAE 20%
none
80
90
26
2
8/10/2014
1CRFme (semi)
0.4
HpaIII
0.2
15 min
CutSmart
1
10
TAE 20%
none
80
90
27
2
8/10/2014
1CRme
0.4
HpaIII
0.2
15 min
CutSmart
1
10
TAE 20%
none
80
90
28
2
8/10/2014
unme
0.4
HpaIII
0.2
15 min
CutSmart
1
10
TAE 20%
none
80
90
29
3
8/12/2014
change in Polyacrylamid gel type to reduce
blurryness of gels
allme
0.4
30
3
8/12/2014
1CRFme (semi)
0.4
none
TBE 20%
none
70V
60
nice sharp bands, restriction enzymes do not cut DNA template completly and something like a oligo dimer was visible.
31
3
8/12/2014
1CRme
0.4
none
TBE 20%
none
70V
60
32
3
8/12/2014
unme
0.4
none
TBE 20%
none
70V
60
33
3
8/12/2014
allme
0.4
none
TBE 20%
none
70V
60
34
3
8/12/2014
1CRFme (semi)
0.4
Sau3AI
0.3
2h
1.1
1
10
TBE 20%
none
70V
60
35
3
8/12/2014
1CRme
0.4
Sau3AI
0.3
2h
1.1
1
10
TBE 20%
none
70V
60
36
3
8/12/2014
unme
0.4
Sau3AI
0.3
2h
1.1
1
10
TBE 20%
none
70V
60
37
3
8/12/2014
allme
0.4
Sau3AI
0.3
2h
1.1
1
10
TBE 20%
none
70V
60
38
3
8/12/2014
1CRFme (semi)
0.4
HpaIII
0.3
2h
1.1
1
10
TBE 20%
none
70V
60
39
3
8/12/2014
1CRme
0.4
HpaIII
0.3
2h
1.1
1
10
TBE 20%
none
70V
60
40
3
8/12/2014
unme
0.4
HpaIII
0.3
2h
1.1
1
10
TBE 20%
none
70V
60
41
3
M
8/12/2014
HpaIII
0.3
2h
1.1
1
10
TBE 20%
none
70V
60
42
4
first activity assay of linear Dnmt1, procedure as seen in “Specificity
of Dnmt1 for Methylation of Hemimethylated CpG Sites Resides in Its Catalytic Domain” by P. Bashtrykov published in Chemistry & Biology 19, 572–578, May 25, 2012 http://www.ncbi.nlm.nih.gov/pubmed/22633409
"
