Team:Paris Saclay/Notebook/September/24
From 2014.igem.org
Contents |
Wednesday 24th September
Lab Work
by Mélanie
Lemon scent
Extraction of plasmid (liquide culture of Yesterday)
and PacI digestion to check another time if we really obtained our plasmid
AND YES it is here ! We had obtained pPS3 and pPS4 !
SalI digestion
In order to obtain pPS5, I digestion by SalI the pPS4 plasmid and the topo-CAD plasmid
ligation
ligation of pPS4 and CAD
(transformation will occure on monday)