Wednesday 3rd September

LabWork

Construction of the fusion protein

We made a classic exttraction of plasmids from the liquid cultures.

Check by electrophoresis if it worked and send it for sequencing.

Lemon scent

by Mélanie

PCR LS

Electrophoresis of the PCR made Yesterday

well 2-3 = PCR with Dream taq or Vent taq

(well 4-5 = checking of the pps5 Extraction)

The PCR have success so I use the PCR clean up kit to purify it

Electrophoresis after purification

Digestion

In order to clone LS in pPSI, i Digest it by PacI

Ligation

We already have some pPSI digested and dephosphorelated

So I do a ligation:

2 hours at room temperature and over night at 4°

pPS5

Plasmid exctraction using the kit (picture is here)

digestion by SalI

Electrophoresis

We saw that we have something strang with our plasmid but we wil check it tomorrow with a good ladder (the ladder here was normally on the well 1)

pPS3 and pPS4

We digest the plasmid by HindIII to direct our insert

well 1 = ladder well 2-3-4 = pPS3 Well 5-6-7 = pPS4

results are very strange

Photo of the Day

[http://iramis.cea.fr/llb/Phocea/Pisp/visu.php?id=35&uid=%20alexei.grinbaum Alexei Grinbaum] , one of those few we have interviewed during this month of september.

To learn more about the ethic aspect of our project, just click here

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