Team:Paris Saclay/Notebook/September/1

Lab Work

B-Construction of the fusion protein

'by Hoang Vu'

We picked out white colonies from Xgal-IPTG petri dish and blue colonies found in non Xgal-IPTG petri dish in a petri dish that contain Ampicillin to check if the blue ones are not came from a carrying of Xgal-IPTG during the precedent pick out.

D-Lemon scent

by melanie

Limone synthase

We had a problem to clone LS in pGMETeasy and pPSI. So from the dish of streaking from 26 AugustWe do PCR of all the clones:

We use the Protocol - PCR for bacterial culture and we use the same PCR condition than on the August 8 but with the appropriate Primer and Biobrick.

PPS5

We check if we have the right insert in our plasmid pPS5 : CAD (cinnalyl alcool deshydrogenase) So we do a PCR in the same condition than August 20

PPS3 and PPS4

We need to work on this plasmid tomorrow so we do some culture from the stock