Team:Paris Saclay/Notebook/August/27

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Contents

1 Wednesday 27th August
- 1.1 Lab Work
  - 1.1.1 D - lemon scent
    - 1.1.1.1 Plasmide extraction
    - 1.1.1.2 PCR of Limonene synthase (BBa762100)

Wednesday 27th August

Lab Work

D - lemon scent

Plasmide extraction

By Mélanie

Extraction of pPS5 with the phenol protocol (as previously described)

PCR of Limonene synthase (BBa762100)

by Mélanie

5 tubes to do a gradient


components	volumes
H₂O	29.75μl
Gotaq buffer	10µl
dNTP 10mM	1µl
iPS67	1µM
iPS66	1µM
DNA	1µl
MgCl2	4µl
Gotaq	1.25µl

PCR cycle
step	temperature (°C)	time
1	95	2min
2	95	30 sec
3	49-55 (gradient)	30sec
4	72	2min
5	72	5min

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