Team:Paris Saclay/Notebook/August/14

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Contents

1 Thursday 14th August
- 1.1 Lab Work
  - 1.1.1 D-Lemon Scent
  - 1.1.2 Transformation of DH5 alpha

Thursday 14th August

Back to the calendar

Lab Work

by Melanie

Preparation of DH5a CaCl2 Competent cell

D-Lemon Scent

by Melanie

From the 20 ml of odor free culture (pJBEI Apra) done yesterday (by Laetitia)

Plasmide extraction without Nucleospin

Transformation of DH5 alpha

by Sean & Eugene We prepare a LB agar environment with a concentration of:

0.5 mM of IPTG
0.008% of Xgal
1/2000 of Ampicilline

and we spread our preparation in a petri dish and incubate overnight at 37°C.

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