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• Home
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  • Detection of Xenobiotics
  • iLOV Characterisation
  • Our Parts
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  • Lab Book
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• 

Lab Book

On this page you can find out more about how our project progressed over time. Our data was originally written in a standard lab book. We have scanned in each page of the book. Each page is listed with along with the page number, the date the page covers, a short description of work carried out that day as well as who did it.

Page No.	Date:	Description:	Carried out by:
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%281%29_.JPG | 1]]	30/06/14	Transformation of iGEM standardised DNA into plasmids for culturing. RBSs and protocols listed.	Martyn Bennet, Beth Hickton
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%282%29_.JPG | 2]]	31/06/14	Transformation of iGEM DNA into plasmids for culturing, cont. List of promoters transformed./td>	Beth Hickton, Edward Muir
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%283%29_.JPG | 3]]	01/07/14	List of promoters transformed, cont. Transformations were plated.	Beth Hickton, Martyn Bennet
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%284%29_.JPG | 4]]	02/07/14	The previous day transformations were plated.	Beth Hickton
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%285%29_.JPG | 5]]	02/07/14	Transformation of iGEM re-suspended promoters.	Beth Hickton
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%286%29_.JPG | 6]]	02/07/14	Preparation of competent E. coli cells	Beth Hickton
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%287%29_.JPG | 7]]	03/07/14	Promoter transformation results.	Jessica Rollit, Martyn Bennet
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%288%29_.JPG | 8]]	03/07/14	E. coli growth rate.	Edward Muir, Beth Hickton
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%289%29_.JPG | 9]]	04/07/14	Transformation of promoters and gene constructs.	Edward Muir, Beth Hickton
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2810%29_.JPG | 10]]	07/07/14	Putting constructs into the iGEM vector and testing for transformation efficiency.	Martyn Bennet, Edward Muir, Beth Hickton
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2811%29_.JPG | 11]]	08/07/14	Repeat construct addition into iGEM vector and testing transformation efficiency.	Edward Muir, Beth Hickton, Martyn Bennet
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2812%29_.JPG | 12]]	08/07/14	Transforming constructs into Top10 and DH5.	Beth Hickton, Martyn Bennet, Edward Muir
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2813%29_.JPG | 13]]	09/07/14	Results of Construction of iGEM vector.	Jessica Rollit, Beth Hickton
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2814%29_.JPG | 14]]	10/07/14	A range of mini-preps	Jessica Rollit, Beth Hickton
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2815%29_.JPG | 15]]	11/07/14	Measuring the length of DNA.	Edward Muir
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2816%29_.JPG | 16]]	11/07/14	Measuring the length of DNA, cont.	Edward Muir
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2817%29_.JPG | 17]]	11/07/14	Mini-prepping picked cultures containing promoters.	Callum Bailey, Elize Hernandez
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2818%29_.JPG | 18]]	11/07/14	Parts sent for sequencing	Callum Bailey, Elize Hernandez
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2819%29_.JPG | 19]]	11/07/14	Linearizing DNA.	Edward Muir
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2820%29_.JPG | 20]]	11/07/14	Creating glycerol stocks.	Beth Hickton, Max Smart
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2821%29_.JPG | 21]]	14/07/14	GFP and RFP resuspension and transformation.	Edward Muir, Martyn Bennet, Beth Hickton
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2822%29_.JPG | 22]]	14/07/14	GFP and RFP resuspension and transformation, cont.	Beth Hickton, Edward Muir
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2823%29_.JPG | 23]]	16/07/14	Mini-prepping GFP and RFP.	Jessica Rollit, Edward Muir, Fran Penrose
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2824%29_.JPG | 24]]	16/07/14	GFP and RFP digestion/ligation to PSB1C3.	Beth Hickton, Edward Muir
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2825%29_.JPG | 25]]	16/07/14	Plasmid digestions.	Edward Muir, Callum Bailey, Benjamin Miller
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2826%29_.JPG | 26]]	16/07/14	RFP/GFP ligations.	Edward Muir
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2827%29_.JPG | 27]]	16/07/14	RFP/GFP ligations, cont.	Edward Muir
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2828%29_.JPG | 28]]	17/07/14	Transformation of GFP/RFP with terminator.	Edward Muir
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2829%29_.JPG | 29]]	18/07/14	Making LB agar for promoters experiment.	Katie Pearce
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2830%29_.JPG | 30]]	18/07/14	Promoter experiment – quantities.	Edward Muir
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2831%29_.JPG | 31]]	18/07/14	Promoter experiment – quantities, cont.	Edward Muir
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2832%29_.JPG | 32]]	18/07/14	Promoter experiment – quantities, cont. 2.	Edward Muir
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2833%29_.JPG | 33]]	21/07/14	Promoter experiment preliminary timetable.	Edward Muir
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2834%29_.JPG | 34]]	22/07/14	Electrophoresis of RFP and GFP (with terminators).	Edward Muir
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2835%29_.JPG | 35]]	22/07/14	Preparation of competent E. coli cells.	Beth Hickton, Peter Reader
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2836%29_.JPG | 36]]	23/07/14	GFP/RFP + terminator liquid broths mini-prepped.	Beth Hickton, Peter Reader, Jessica Rollit
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2837%29_.JPG | 37]]	23/07/14	Potassium Chromate preparation.	Peter Reader, Benjamin Miller, Beth Hickton
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2838%29_.JPG | 38]]	23/07/14	Transformation of other promoters.	Edward Muir
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2839%29_.JPG | 39]]	24/07/14	Moving GFP+T and RFP+T onto AMP backbones.	Edward Muir, Katie Pearce
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2840%29_.JPG | 40]]	24/07/14	Ligation of GFP+T and RFP+T onto AMP backbones.	Edward Muir, Beth Hickton
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2841%29_.JPG | 41]]	24/07/14	Potassium Dichromate colorimetry and spectrophotometry.	Beth Hickton, Edward Muir
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2842%29_.JPG | 42]]	28/07/14	Plating Anderson RBSs.	Beth Hickton, Edward Muir, Elize Hernandez
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2843%29_.JPG | 43]]	28/07/14	Mini-prep of reporters, promoters and RBSs.	Beth Hickton, Elize Hernandez, Edward Muir
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2844%29_.JPG | 44]]	29/07/14	COSHH Form created for Nitroglycerine experiments.	Beth Hickton, Peter Reader, Benjamin Miller
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2845%29_.JPG | 45]]	30/07/14	Cr(VI) / Ligand experimentation.	Peter Reader
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2846%29_.JPG | 46]]	31/07/14	Mini-prep of constructs.	Edward Muir, Elize Hernandez, Beth Hickton
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2847%29_.JPG | 47]]	31/07/14	Digestion and ligation of promoter/RBS combos.	Edward Muir, Callum Bailey
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2848%29_.JPG | 48]]	31/07/14	Oligo preparation.	Edward Muir, Callum Bailey
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2849%29_.JPG | 49]]	31/07/14	Ligation of promoter and RBs digestions.	Edward Muir, Callum Bailey, Benjamin Miller
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2850%29_.JPG | 50]]	01/08/14	Digestion and Ligation of constructs 004 and 007.	Edward Muir, Jessica Rollit, Beth Hickton
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2851%29_.JPG | 51]]	01/08/14	Digestion and Ligation of constructs 004 and 007, cont.	Edward Muir
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2852%29_.JPG | 52]]	05/08/14	Colonies picked and mini-prepped.	Callum Bailey
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2853%29_.JPG | 53]]	05/08/14	Colonies picked and mini-prepped, cont.	Callum Bailey
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2854%29_.JPG | 54]]	06/08/14	Testing promoter/RBS combination efficiency.	Callum Bailey, Edward Muir
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2855%29_.JPG | 55]]	06/08/14	Testing promoter/RBS combination efficiency, cont.	Callum Bailey, Edward Muir
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2856%29_.JPG | 56]]	07/08/14	Picking colonies from promoter/RBS combination transformations.	Edward Muir, Callum Bailey
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2857%29_.JPG | 57]]	07/08/14	T. Cam experiment.	Edward Muir, Callum Bailey
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2858%29_.JPG | 58]]	11/08/14	Promoter/RBS combinations again.	Edward Muir, Callum Bailey
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2859%29_.JPG | 59]]	11/08/14	Promoter/RBS combinations again, cont.	Edward Muir, Callum Bailey
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2860%29_.JPG | 60]]	11/08/14	Inoculation of enzymes for construct 001 and 003.	Edward Muir, Callum Bailey
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2861%29_.JPG | 61]]	12/08/14	Inoculation of enzymes for construct 001 and 003, cont.	Edward Muir, Callum Bailey
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2862%29_.JPG | 62]]	12/08/14	Transformation of a sample promoter/RBS combination.	Callum Bailey
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2863%29_.JPG | 63]]	12/08/14	T. Cam experiment for solvents and ether.	Edward Muir
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2864%29_.JPG | 64]]	13/08/14	Combination transformation results and construct testing.	Edward Muir
[[media:Exeter_iGEM_2014_Lab_Book_Write-Up_%2865%29_.JPG | 65]]	13/08/14	Putting oligos into GFP and RFP.	Edward Muir

These lab book photos are a record of how we produced our synthetic bacterium. All experiments after this point are recorded in their relevant lab reports.

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