Protocols

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• Deadline: 08/oct.
  • Uniformisation des textes.
  • Rappeler Fabio de creer la photo pour le protocol BioBrick Assembly
  • Changer les enzymes du protocol
  • Text explicatif des comment et pourquoi on a fait ces protocols
• Deadline: 12/oct
  • Final review.

1. Preparation of supercompetent E. coli cells
2. Transformation of CaCl2 supercompetent E. coli cells
3. Extraction of the Plasmid DNA from Bacteria by using NucleoSpin® Plasmid
4. Extraction of the Plasmid DNA from Bacteria without NucleoSpin® Plasmid
5. Gel Electrophoresis
6. Transformation of CaCl2 supercompetent E. coli cells
7. PCR for the genomic DNA of bacteria
8. PCR clean-up
9. PCR for bacterial culture
10. Transformation of competent cells by electroporation
11. BioBrick Assembly
12. P1 Phages Stock
13. P1 phages transduction
14. Transformation of competent E. coli by CaCl₂
15. Cloning in pGEMTeasy
16. Electro elution of DNA from agarose gel