Team:Paris Saclay/Notebook/September/15

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Contents

1 Lab Work
- 1.1 lemon scent
- 1.2 Construction of the fusion protein (color)
  - 1.2.1 PCR
  - 1.2.2 transformation for stock of the w6 clone

Lab Work

by Mélanie

lemon scent

Transformation with the ligation (futur pPS2/4)

liquide culture of some PS clone (topo)

Transformation of LS GS CAS (topo) to do stock

PCR pSCBC3 cloning

PCR of GS : primer with ecoRI and PSTI restriction site

Construction of the fusion protein (color)

PCR

We have decided to separate the two protein So I do a PCR to have the yellow and the blue protein and i cloned them in a topo plasmid.

transformation for stock of the w6 clone

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