Friday 1st August

Lab work

A - The frame coli Odor free

Stria on LB dishes

by Romain

Strains used: MG1655 and MG1655Z1, transformed the 30th July, striated on 4 LB dishes per strain.

C - Salicylate Inducible Suppressing System

Liquid Culture

by Fabio

8 liquid cultures with 3ml of LB and 3µl of amp (at 10am - 8°C), all from the colonies made the 31st July. The colonies will be put at 37°C on sunday 3rd in order to have grown colonies on monday.

D - Lemon scent

Results of the Transformation of electrocompetent cells

by Arnaud & Terry

Transformation of electrocompetent cells made the July 29th. Strains used: DY330. Plasmid used: pJBEI-6409 (code for enzymes to produce limonene from glucose in E. coli)

Results:

• Nothing has grown on the control dishes.
• Failure, nothing has grown on the dishes which contain DY330 with pJBE1

New Transformation of electrocompetent cells

by Terry

Strain used: DY330. Plasmid used: pJBEI-6409 (code for enzymes to produce limonene from glucose in E. coli)

Make 3 électroporations in cold electroporation cuvettes:

• A control cuvette(without DNA): 50µl of DY330.
• A second cuvette: 50µl of DY330 culture + 2µl of pJBEI-6409 plasmid.
• A third cuvette: 50µl of DY330 culture + 0.5µl of pJBEI-6409 plasmid.

Electroporation : 2500V, 132W, 40µF.

After that, add 1ml of cold LB in each cuvette and transfer in tubes to incubate during 1 to 2h at 30°C.

Spread on 7 dishes LB + Cm:

• 100µl of control (without plasmid)
• 50µl of transformed DY330 with 0.5µl of pJBEI-6409
• 100µl of transformed DY330 with 0.5µl of pJBEI-6409
• The rest of transformed DY330 with 0.5µl pJBEI-6409
• 50µl of transformed DY330 with 2µl of pJBEI-6409
• 100µl of transformed DY330 with 2µl of pJBEI-6409
• The rest of transformed DY330 with 2µl pJBEI-6409

Incubate for 2 days at 30°C.

Members there:

• Instructors and advisors: Alice, Solenne and Sylvie.
• Students: Arnaud, Fabio, Romain, Sean and Terry.

Back to the calendar