Team:Paris Saclay/Notebook/September/10
From 2014.igem.org
Contents |
Wednesday 10th September
Lab Work
by Mélanie
D-Lemon Scent
Plasmid extraction
From the liquide culture made Yesterday I do a plasmid extraction.
these clones (LS GS CAD) had sent to sequencing problem with ps
digestion
I digest with PacI the plasmid that contain LS and GS
component | volume |
---|---|
H2O | 5μl |
buffer | 3μl |
PacI | 1μl |
Plasmid | 20μl |
Purification
Electrophoresis to isolate the insert and purification with a kit = I failed
culture
As we failed to obtain two right extraction for LS, I try another clone so I do a liquide culture of the clones A and B
Photo of the Day
[http://emmanuelhirsch.fr/ Emmanuel Hirsch] , one of those few we have interviewed during this month of september.
To learn more about the ethic aspect of our project, just click here
Modeling
by Arnaud, Eugène, Juliette and Pierre
Meeting about modeling with advisor Claire to talk about the achievement of our model
- Diffusion of O2 in the gel : maths is OK but biologist need more drawings.
- Bacterial growth on the surface : because of the new aspect of our project, we have to change our point of view. Tomorrow, a real discussion with the biologists is needed to understand more precisely what they are doing in the lab.
- Translecture system : maybe we have to give up this part of our work because of the new project.
- Diffusion of the limonene : Eugène has focused on the concentration of limonene in the atmosphere.