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Team:Paris Saclay/Notebook/August/27
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| + | ==== Electrophoresis of pPS3 and pPS4==== | ||
| + | ''by Terry'' | ||
| + | Alice, donne moi la photo. | ||
{{Team:Paris_Saclay/notebook_footer}} | {{Team:Paris_Saclay/notebook_footer}} | ||
Revision as of 17:01, 27 August 2014
Contents |
Wednesday 27th August
Lab Work
B - Construction of the fusion protein (color)
PCR clean-up of colonies containing pGEMTeasy+chromoprotein
by Sean
Three colonies were selected from the gel prepared earlier by Laetitia, namely #3,#4, and #6. The three gel samples underwent PCR clean-up.
Gel electrophoresis of PCR clean-up results containing pGEMTeasy+chromoprotein
by Sean
PCR clean-up was performed ealier today. 1µl of each result was used for the electrophoresis. Nothing appeared under UV.
D - lemon scent
Plasmide extraction
By Mélanie
Extraction of pPS5 with the phenol protocol (as previously described)
PCR of Limonene synthase (BBa762100)
by Mélanie
5 tubes to do a gradient
| components | volumes |
|---|---|
| H2O | 29.75μl |
| Gotaq buffer | 10µl |
| dNTP 10mM | 1µl |
| iPS67 | 1µM |
| iPS66 | 1µM |
| DNA | 1µl |
| MgCl2 | 4µl |
| Gotaq | 1.25µl |
| step | temperature (°C) | time |
|---|---|---|
| 1 | 95 | 2min |
| 2 | 95 | 30 sec |
| 3 | 49-55 (gradient) | 30sec |
| 4 | 72 | 2min |
| 5 | 72 | 5min |
Electrophoresis of pPS3 and pPS4
by Terry
Alice, donne moi la photo.
