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Team:Paris Saclay/Notebook/July/8
From 2014.igem.org
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[https://2014.igem.org/Team:Paris_Saclay/Protocols/Transformation_of_supercompetent_Ecoli_cells Protocol] | [https://2014.igem.org/Team:Paris_Saclay/Protocols/Transformation_of_supercompetent_Ecoli_cells Protocol] | ||
| - | NB : The protocol was modified. Instead of spreading 10-1 and 10-2 solutions of bacteria we took a non diluted solution and a 10-1 solution. | + | NB : The protocol was modified. Instead of spreading 10<sup>-1</sup> and 10<sup>-2</sup> solutions of bacteria we took a non diluted solution and a 10<sup>-1</sup> solution. |
====Plasmid DNA preparation:==== | ====Plasmid DNA preparation:==== | ||
Revision as of 14:15, 26 August 2014
Contents |
Tuesday 8th July
Lab work
Rehydration of the following biobricks:
by Sean
- BBa_B0015(CmR)
- BBa_J23119(CmR)
- BBa_K731020(CmR)
- BBa_K206000(CmR)
- BBa_J04500(CmR)
- BBa_J23100(AmpR)
- BBa_J23106(AmpR)
- BBa_J23114(AmpR)
Protocol:
- Pierce foil cover of well containing biobrick of interest.
- Add 10 μl of water in well.
- Transfer contents of well into a 1.5 ml microcentrifuge tube.
Transformation in DH5α of the following biobricks:
by Mathieu & Maher
- BBa_B0015(CmR)
- BBa_J23119(CmR)
- BBa_K731020(CmR)
- BBa_K206000(CmR)
- BBa_J04500(CmR)
- BBa_J23100(AmpR)
- BBa_J23106(AmpR)
- BBa_J23114(AmpR)
- Control + : pUC18 (20ng)
- Control -
NB : The protocol was modified. Instead of spreading 10-1 and 10-2 solutions of bacteria we took a non diluted solution and a 10-1 solution.
Plasmid DNA preparation:
by Romain & Sean
Plasmids used:
- pJBEI-6409
E. coli strain used:
- XL1-Blue
Members present:
- Instructors and advisors: Alice, Solenne and Sylvie.
- Students: Arnaud, Fabio, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain and Sean.
