Revision as of 09:32, 22 August 2014

Thursday 21st August

by Sean

by Huang vu and laetitia

We extracted the DNA from the agarose band saves yesterday

By Mélanie

From preculture of bacteria with pPSI we extract the plasmid

By Mélanie

Following the cloning made by Laeticia in pGMETeasy, i extract the plasmid (protocol)

and I do some stock of each stain

by Eugene

We have made an electrophoresis of purified CAD and pMCS5.

30 min incubation in 37°c

night at 16°c

@@ Line 9: / Line 9: @@
 ==== Electro elution of pPS1 digested by Pac1 ====
-"by Huang vu and laetitia"
+''by Huang vu and laetitia''
 We extracted the DNA from the agarose band saves yesterday
 [https://2014.igem.org/Team:Paris_Saclay/Protocols/Electro_extraction_of_DNA_from_agarose_gel protocole of electro elution]
+====Extraction of pPSI====
+'' By Mélanie''
+From preculture of bacteria with pPSI we extract the plasmid
+[https://2014.igem.org/Team:Paris_Saclay/Protocols/Extraction_of_the_Genomic_DNA_from_Bacteria_without_NucleoSpin%C2%AE_Tissue protocol]
+====Extraction of pGMETeasy with LS GS and PS====
+''By Mélanie''
+Following the cloning made by Laeticia in pGMETeasy, i extract the plasmid ([https://2014.igem.org/Team:Paris_Saclay/Protocols/Extraction_of_the_Genomic_DNA_from_Bacteria_by_using_NucleoSpin%C2%AE_Tissue protocol])
+and I do some stock of each stain
 ==== Ligation of CAD in pMCS5 ====