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Revision as of 13:44, 20 August 2014 (view source) MelaCriq (Talk | contribs) (→Bacterial culture) ← Older edit		Revision as of 14:33, 20 August 2014 (view source) MelaCriq (Talk | contribs) (→Bacterial culture) Newer edit →
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	Preculture 20ml of DH5a pPSI (apra 1/2000)		Preculture 20ml of DH5a pPSI (apra 1/2000)
		+
		+
		+	===CAD PCR===
		+	Today we have receive our synthetic gene CAD (cinnamyl alcohol deshydrogenase) :)
		+
		+	so we do a PCR (with go taq):
		+
		+	23.25 µl --> H2O
		+
		+	10 µl --> 5xbuffer
		+
		+	1 µl --> dNTP
		+
		+	4 µl --> MgCl2
		+
		+	5 µl iPS 79 (Primer)
		+
		+	5 µl iPS 80
		+
		+	Very small quantity of DNA
		+
		+	1.5 µl --> Gotaq
		+
		+	0.25

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Revision as of 14:33, 20 August 2014

D- Lemon scent

Bacterial culture

by Laetitia

Here are the results of the bacterial culture of yesterday

Bacteria with the pGEMTeasy without the DNA insert are blue and the bacteria with the pGEMTeasy and the DNA insert (PS, GS or LS gene) are white

Then, we have chosen 3 white colonies for each DNA insert and we have launched 3 corresponding liquid cultures :

- 5mL LB + ampiciline (1/1000e)

- 1 white colony

The 9 cultures are incubated at 37°C

by Melanie

Preculture 20ml of DH5a pPSI (apra 1/2000)

CAD PCR

Today we have receive our synthetic gene CAD (cinnamyl alcohol deshydrogenase) :)

so we do a PCR (with go taq):

23.25 µl --> H2O

10 µl --> 5xbuffer

1 µl --> dNTP

4 µl --> MgCl2

5 µl iPS 79 (Primer)

5 µl iPS 80

Very small quantity of DNA

1.5 µl --> Gotaq

0.25