Team:Paris Saclay/Notebook/August/19
From 2014.igem.org
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=Tuesday 19th August= | =Tuesday 19th August= | ||
==Lab work== | ==Lab work== | ||
+ | |||
+ | ===D - Lemon Scent=== | ||
+ | |||
+ | ==== Digestion of PCR results ==== | ||
+ | ''by Sean, Hoang Vu, Eugene'' | ||
+ | |||
+ | {| class="wikitable centre" width="50%" | ||
+ | |+ | ||
+ | |- | ||
+ | ! scope=col | components | ||
+ | ! scope=col | volumes | ||
+ | |- | ||
+ | |PCR purified LS | ||
+ | |15 μl | ||
+ | |- | ||
+ | |FastDigest green buffer 10X | ||
+ | |2 μl | ||
+ | |- | ||
+ | |PacI | ||
+ | |1 µl | ||
+ | |- | ||
+ | |H<sub>2</sub>O | ||
+ | |2 µl | ||
+ | |} | ||
+ | |||
+ | {| class="wikitable centre" width="50%" | ||
+ | |+ | ||
+ | |- | ||
+ | ! scope=col | components | ||
+ | ! scope=col | volumes | ||
+ | |- | ||
+ | |PCR purified GS | ||
+ | |15 μl | ||
+ | |- | ||
+ | |FastDigest green buffer 10X | ||
+ | |2 μl | ||
+ | |- | ||
+ | |PacI | ||
+ | |1 µl | ||
+ | |- | ||
+ | |H<sub>2</sub>O | ||
+ | |2 µl | ||
+ | |} | ||
+ | |||
+ | {| class="wikitable centre" width="50%" | ||
+ | |+ | ||
+ | |- | ||
+ | ! scope=col | components | ||
+ | ! scope=col | volumes | ||
+ | |- | ||
+ | |PCR purified PS | ||
+ | |15 μl | ||
+ | |- | ||
+ | |FastDigest green buffer 10X | ||
+ | |2 μl | ||
+ | |- | ||
+ | |PacI | ||
+ | |1 µl | ||
+ | |- | ||
+ | |H<sub>2</sub>O | ||
+ | |2 µl | ||
+ | |} | ||
+ | |||
+ | |||
+ | {| class="wikitable centre" width="50%" | ||
+ | |+ | ||
+ | |- | ||
+ | ! scope=col | components | ||
+ | ! scope=col | volumes | ||
+ | |- | ||
+ | |PCR purified pPS1 | ||
+ | |30 μl | ||
+ | |- | ||
+ | |FastDigest green buffer 10X | ||
+ | |4 μl | ||
+ | |- | ||
+ | |PacI | ||
+ | |2 µl | ||
+ | |- | ||
+ | |H<sub>2</sub>O | ||
+ | |4 µl | ||
+ | |} | ||
+ | |||
+ | |||
+ | |||
+ | |||
{{Team:Paris_Saclay/notebook_footer}} | {{Team:Paris_Saclay/notebook_footer}} |
Revision as of 14:29, 19 August 2014
Contents |
Tuesday 19th August
Lab work
D - Lemon Scent
Digestion of PCR results
by Sean, Hoang Vu, Eugene
components | volumes |
---|---|
PCR purified LS | 15 μl |
FastDigest green buffer 10X | 2 μl |
PacI | 1 µl |
H2O | 2 µl |
components | volumes |
---|---|
PCR purified GS | 15 μl |
FastDigest green buffer 10X | 2 μl |
PacI | 1 µl |
H2O | 2 µl |
components | volumes |
---|---|
PCR purified PS | 15 μl |
FastDigest green buffer 10X | 2 μl |
PacI | 1 µl |
H2O | 2 µl |
components | volumes |
---|---|
PCR purified pPS1 | 30 μl |
FastDigest green buffer 10X | 4 μl |
PacI | 2 µl |
H2O | 4 µl |