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Team:Paris Saclay/Notebook/August/18
From 2014.igem.org
(Difference between revisions)
(→D - Lemon Scent) |
(→PCR of BBa_K517003) |
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''By Hoang Vu and Eugène'' | ''By Hoang Vu and Eugène'' | ||
| - | We realized a PCR to | + | We realized a PCR to amplify the β-pinene synthase gene. |
| - | #H2O - 29.5 | + | #H2O - 29.5 µl |
| - | #Buffer 5X - 10 | + | #Buffer 5X - 10 µl |
| - | #MgCl2 - 4 | + | #MgCl2 - 4 µl |
| - | #dNTP - | + | #dNTP - 1µl |
| - | #IPS68bis (25µM) - | + | #IPS68bis (25µM) - 2µl |
| - | #IPS69 (25µM) - | + | #IPS69 (25µM) - 2µl |
| - | #DNA - | + | #DNA - 1µl |
| - | #GoTag enzyme - 0. | + | #GoTag enzyme - 0.5µl |
====PCR of BBa_K762100==== | ====PCR of BBa_K762100==== | ||
Revision as of 08:16, 19 August 2014
Contents |
Monday 18th August
Lab work
D - Lemon Scent
PCR of BBa_K517003
By Hoang Vu and Eugène
We realized a PCR to amplify the β-pinene synthase gene.
- H2O - 29.5 µl
- Buffer 5X - 10 µl
- MgCl2 - 4 µl
- dNTP - 1µl
- IPS68bis (25µM) - 2µl
- IPS69 (25µM) - 2µl
- DNA - 1µl
- GoTag enzyme - 0.5µl
PCR of BBa_K762100
Five tubes were prepared. Protocol was modified to match the one used for BBa_K517003 and p cola (PCR conducted on 8th August).
Primers used: iPS66 and iPS67.
