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Team:Paris Saclay/Protocols
From 2014.igem.org
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| - | #[https://2014.igem.org/Team:Paris_Saclay/Protocols/Preparation_of_supercompetent_Ecoli_cells Preparation of supercompetent E.coli cells] | + | #[https://2014.igem.org/Team:Paris_Saclay/Protocols/Preparation_of_supercompetent_Ecoli_cells Preparation of supercompetent ''E. coli'' cells] |
| - | #[https://2014.igem.org/Team:Paris_Saclay/Protocols/Transformation_of_supercompetent_Ecoli_cells Transformations of supercompetent E. coli cells] | + | #[https://2014.igem.org/Team:Paris_Saclay/Protocols/Transformation_of_supercompetent_Ecoli_cells Transformations of supercompetent ''E. coli'' cells] |
#[https://2014.igem.org/Team:Paris_Saclay/Protocols/Extraction_of_the_Genomic_DNA_from_Bacteria_by_using_NucleoSpin%C2%AE_Tissue Extraction of the Genomic DNA from Bacteria by using NucleoSpin® Tissue] | #[https://2014.igem.org/Team:Paris_Saclay/Protocols/Extraction_of_the_Genomic_DNA_from_Bacteria_by_using_NucleoSpin%C2%AE_Tissue Extraction of the Genomic DNA from Bacteria by using NucleoSpin® Tissue] | ||
#[https://2014.igem.org/Team:Paris_Saclay/Protocols/Extraction_of_the_Genomic_DNA_from_Bacteria_without_NucleoSpin®_Tissue Extraction of the Genomic DNA from Bacteria without NucleoSpin® Tissue] | #[https://2014.igem.org/Team:Paris_Saclay/Protocols/Extraction_of_the_Genomic_DNA_from_Bacteria_without_NucleoSpin®_Tissue Extraction of the Genomic DNA from Bacteria without NucleoSpin® Tissue] | ||
#[https://2014.igem.org/Team:Paris_Saclay/Protocols/Gel_Electrophoresis Gel Electrophoresis] | #[https://2014.igem.org/Team:Paris_Saclay/Protocols/Gel_Electrophoresis Gel Electrophoresis] | ||
| - | #[https://2014.igem.org/Team:Paris_Saclay/Protocols/Transformation_of_supercompetent_Ecoli_cells_with_CaCl2 Transformation of CaCl<sub>2</sub> supercompetent E.coli cells] | + | #[https://2014.igem.org/Team:Paris_Saclay/Protocols/Transformation_of_supercompetent_Ecoli_cells_with_CaCl2 Transformation of CaCl<sub>2</sub> supercompetent ''E. coli'' cells] |
#[https://2014.igem.org/Team:Paris_Saclay/Protocols/PCR_for_the_genomic_DNA_of_bacteria PCR for the genomic DNA of bacteria] | #[https://2014.igem.org/Team:Paris_Saclay/Protocols/PCR_for_the_genomic_DNA_of_bacteria PCR for the genomic DNA of bacteria] | ||
#[https://2014.igem.org/Team:Paris_Saclay/Protocols/PCR_clean-up PCR clean-up] | #[https://2014.igem.org/Team:Paris_Saclay/Protocols/PCR_clean-up PCR clean-up] | ||
| Line 15: | Line 15: | ||
#[https://2014.igem.org/Team:Paris_Saclay/Protocols/P1_phages_stock P1 Phages Stock] | #[https://2014.igem.org/Team:Paris_Saclay/Protocols/P1_phages_stock P1 Phages Stock] | ||
#[https://2014.igem.org/Team:Paris_Saclay/Protocols/P1_phages_transduction P1 phages transduction] | #[https://2014.igem.org/Team:Paris_Saclay/Protocols/P1_phages_transduction P1 phages transduction] | ||
| - | #[https://2014.igem.org/Team:Paris_Saclay/Protocols/Transformation_of_competent_e.coli_by_CaCl2 Transformation of competent | + | #[https://2014.igem.org/Team:Paris_Saclay/Protocols/Transformation_of_competent_e.coli_by_CaCl2 Transformation of competent ''E. coli'' by CaCl2] |
{{Team:Paris_Saclay/protocols_footer}} | {{Team:Paris_Saclay/protocols_footer}} | ||
Revision as of 18:58, 14 August 2014
Protocols
- Preparation of supercompetent E. coli cells
- Transformations of supercompetent E. coli cells
- Extraction of the Genomic DNA from Bacteria by using NucleoSpin® Tissue
- Extraction of the Genomic DNA from Bacteria without NucleoSpin® Tissue
- Gel Electrophoresis
- Transformation of CaCl2 supercompetent E. coli cells
- PCR for the genomic DNA of bacteria
- PCR clean-up
- PCR for bacterial culture
- Transformation of competent cells by electroporation
- BioBrick Assembly
- P1 Phages Stock
- P1 phages transduction
- Transformation of competent E. coli by CaCl2
