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Team:Paris Saclay/Notebook/August/14
From 2014.igem.org
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Plasmide extraction without Nucleospin | Plasmide extraction without Nucleospin | ||
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| + | === Transformation of DH5 alpha === | ||
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| + | We prepare a LB agar environment with a concentration of: | ||
| + | * 0.5 mM of IPTG | ||
| + | * 0.008% of Xgal | ||
| + | * 1/2000 of Ampicilline | ||
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| + | and we spread our preparation in a petri dish and incubate overnight at 37°C. | ||
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[https://2014.igem.org/Team:Paris_Saclay/Protocols/Extraction_of_the_Genomic_DNA_from_Bacteria_without_NucleoSpin%C2%AE_Tissue Protocol] | [https://2014.igem.org/Team:Paris_Saclay/Protocols/Extraction_of_the_Genomic_DNA_from_Bacteria_without_NucleoSpin%C2%AE_Tissue Protocol] | ||
Revision as of 13:56, 14 August 2014
Contents |
Thursday 14th August
Lab Work
by Melanie
Preparation of DH5a CaCl2 Competent cell
D-Lemon Scent
by Melanie
From the 20 ml of odor free culture (pJBEI Apra) done yesterday (by Laetitia)
Plasmide extraction without Nucleospin
Transformation of DH5 alpha
We prepare a LB agar environment with a concentration of:
- 0.5 mM of IPTG
- 0.008% of Xgal
- 1/2000 of Ampicilline
and we spread our preparation in a petri dish and incubate overnight at 37°C.
