Team:Paris Saclay/Protocols/PCR clean-up
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# After the PCR, pool your PCR result in an eppendorf 1,5ml. | # After the PCR, pool your PCR result in an eppendorf 1,5ml. | ||
# Add 200µl NTI. | # Add 200µl NTI. | ||
- | # Centrifuge at 11000g, 30 | + | # Centrifuge at 11000g, 30 seconds RT. |
# Discard the supernatant, wash first time with 700µl NT3. | # Discard the supernatant, wash first time with 700µl NT3. | ||
# Centrifuge at 11000g, 30 secondes RT. | # Centrifuge at 11000g, 30 secondes RT. | ||
# Discard the supernatant, wash a second time with 700µl NT3. | # Discard the supernatant, wash a second time with 700µl NT3. | ||
- | # Centrifuge at 11000g, 30 | + | # Centrifuge at 11000g, 30 seconds RT. |
# Centrifuge the column empty. | # Centrifuge the column empty. | ||
# Add 20µl MilliQ water and centrifuge at 11000g, 1 min RT. | # Add 20µl MilliQ water and centrifuge at 11000g, 1 min RT. | ||
# Collect your DNA in an eppendorf 1,5ml. | # Collect your DNA in an eppendorf 1,5ml. | ||
{{Team:Paris_Saclay/protocols_footer}} | {{Team:Paris_Saclay/protocols_footer}} |
Revision as of 11:51, 11 August 2014
PCR clean-up
- After the PCR, pool your PCR result in an eppendorf 1,5ml.
- Add 200µl NTI.
- Centrifuge at 11000g, 30 seconds RT.
- Discard the supernatant, wash first time with 700µl NT3.
- Centrifuge at 11000g, 30 secondes RT.
- Discard the supernatant, wash a second time with 700µl NT3.
- Centrifuge at 11000g, 30 seconds RT.
- Centrifuge the column empty.
- Add 20µl MilliQ water and centrifuge at 11000g, 1 min RT.
- Collect your DNA in an eppendorf 1,5ml.