Thursday 7th August

Lab work

C - Salicylate Inducible Suppressing System

DNA extraction from gel agarose

In progress

D - Lemon Sent

PCR of pCola plasmid (Geranyl Synthase)

by Melanie

we use the same protocol as yesterday but we have done 8 tubes to apply a gradient in the thermocycleur during the third step:

1. 57°
2. 56.3°
3. 55.1°
4. 53.3°
5. 51°
6. 49.3°
7. 47.9°
8. 47°

PCR protocol: 98° --> 2min

5 PCR cycle:

25 PCR cycle

and last step: 72° during 10min

but we don't have any results

Test the odor of e.coli with pJBEI6409 plasmide

'by melanie' Transformation of competent cells 5mg1655) by electroporation (pJBEI6409) [1] We do some stock

Extraction of the Genomic DNA

by Juliette & Terry

• APRA PJBEI cl. 1
• APRA PJBEI cl. 2
• APRA PJBEI cl. 3

From liquid culture made the 6th August.

Protocol

Polymerase chain reaction

by Sean & Pierre

for this PCR, five tubes of each of the following Bio-bricks were prepared.

BBa_K517003

BBa_K762100

Tubes were placed in PCR machine with the following parameters.

Members present:

• Instructors and advisors: Alice.
• Students: Eugene, Fabio, Hoang Vu, Juliette, Melanie, Pierre, Romain, Sean and Terry.

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