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Team:Paris Saclay/Notebook/July/3
From 2014.igem.org
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=Thursday 3rd July= | =Thursday 3rd July= | ||
==Lab Work== | ==Lab Work== | ||
| - | ===Phages P1 transduction in MG1655 and MG1655Z1=== | + | ===A - The chassis coli Odor free=== |
| + | ====Phages P1 transduction in MG1655 and MG1655Z1==== | ||
''by Mathias'' | ''by Mathias'' | ||
Revision as of 12:45, 7 August 2014
Contents |
Thursday 3rd July
Lab Work
A - The chassis coli Odor free
Phages P1 transduction in MG1655 and MG1655Z1
by Mathias
In 2ml Eppendorf :
- 4 eppendorf MG1655 (0.1ml + 50µl CaCl)
- 4 eppendorf MG1655Z1 (0.1ml + 50µl CaCl)
Add 100µl of the different phages. Wait 20 minutes!
Add in each eppendorf : 1ml LB liquid + 500µl citrate at 5.10^-3M Wait 1 or 2 hours at 37°C with agitation
Pour in LB Kan petri dishes, incubate at 37°C. (Turn the petri dishes over when there is no more liquid in!)
Plasmid DNA Purification
by Fabio, Maher, Mathieu, Marie and Romain
- BBa_K773002 (x2)
- BBa_K517003 (x2)
- BBa_K773002 (x2)
- BBa_K592011 (x2)
- BBa_K1033910 (x2)
- BBa_K592009 (x2)
- BBa_K786002 (x2)
- BBa_K786003 (x2)
- BBa_J45014 (x2)
- BBa_I9026 (x2)
Members present:
- Instructors : Alice & Solenne.
- Students : Arnaud, Fabio, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain.
