Team:Paris Saclay/Notebook/June/30

From 2014.igem.org

(Difference between revisions)
(→2 - Réhydration of selected biobricks:)
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{{Team:Paris_Saclay/notebook_header}}
=Monday 30th June=
=Monday 30th June=
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==Lab work==
==Lab work==
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====Culture or the supercompetents====
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====1 - Culture or the supercompetents:====
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''by Mathias''
''by Mathias''
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1ml of an overnight culture in 100ml of LB.
1ml of an overnight culture in 100ml of LB.
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====2 - Réhydration of selected biobricks:====
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====Réhydration of selected biobricks====
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''by Juliette''
''by Juliette''
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* BBa_K517003 (lemon scent, β-pinene)
* BBa_K517003 (lemon scent, β-pinene)
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====3 - Transformations :====
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====Transformations====
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''by Maher & Mathias''
''by Maher & Mathias''
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Spread on petri dishes with the concentration 10<sup>-1</sup> and 10<sup>-2</sup>.
Spread on petri dishes with the concentration 10<sup>-1</sup> and 10<sup>-2</sup>.
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====4 - Preparations :====
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====Preparations====
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''by Marie and Mathieu''
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* Aliquots of antibiotics :
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* Aliquots of antibiotics
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''by Marie & Mathieu''
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Put 0.203g of Chloramphenicol in 10ml of ethanol. Aliquots of 1ml.
Put 0.203g of Chloramphenicol in 10ml of ethanol. Aliquots of 1ml.
(Do the same for Kanamycin and Ampicillin)
(Do the same for Kanamycin and Ampicillin)
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''by Romain''
* Petri dishes
* Petri dishes
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''by Romain''
 
Add 250µl of antibiotics in 250ml of LB. Pour in petri dishes.
Add 250µl of antibiotics in 250ml of LB. Pour in petri dishes.
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''by Arnaud''
* Range of gelose with different concentration of agar
* Range of gelose with different concentration of agar
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''by Arnaud''
 
Add in 6 bottles of 500ml 2g of LB (lysogeny broth), and in each bottle add successively 1500mg (15mg/ml), 2000mg (20mg/ml), 2500mg (25mg/ml), 3000mg (30mg/ml), 3500mg (35mg/ml) and 4000mg (40mg/ml) of agar. And just before autoclaving complete each bottle with 100ml of reverse osmosis-purified water.
Add in 6 bottles of 500ml 2g of LB (lysogeny broth), and in each bottle add successively 1500mg (15mg/ml), 2000mg (20mg/ml), 2500mg (25mg/ml), 3000mg (30mg/ml), 3500mg (35mg/ml) and 4000mg (40mg/ml) of agar. And just before autoclaving complete each bottle with 100ml of reverse osmosis-purified water.
'''Members present:'''
'''Members present:'''
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* Instructors : Solenne.
* Instructors : Solenne.
* Students : Arnaud, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain.
* Students : Arnaud, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain.
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{{Team:Paris_Saclay/notebook_footer}}
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[https://2014.igem.org/Team:Paris_Saclay/Notebook Back to the calendar]
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Revision as of 08:59, 7 August 2014

Contents

Monday 30th June

Lab work

Culture or the supercompetents

by Mathias

  • MG1655
  • MG1655Z1

1ml of an overnight culture in 100ml of LB.

Réhydration of selected biobricks

by Juliette

  • BBa_I9026 (rhII translational unit)
  • BBa_K592025 (BB0034-amilCP)
  • BBa_K786002 (sensory rhodopsin II & Tar sensitive to blue-green light)
  • BBa_K786003 (sensory rhodopsin I & Tar sensitive to orange light)
  • BBa_K778002
  • BBa_K592011 (cjBlue green chromoprotein)(2 times)
  • BBa_K592009 (amilCP blue chromoprotein)
  • BBa_K103391 (Yellow chromoprotein and Lime yellow-green chromoprotein)(2 times)
  • BBa_J45014 (lemon scent, alcohol acetyltransferase I)
  • BBa_K517003 (lemon scent, β-pinene)

Transformations

by Maher & Mathias

We use the DH5α provided by our advisors for the aforementioned Biobricks. Spread on petri dishes with the concentration 10-1 and 10-2.

Preparations

by Marie and Mathieu

  • Aliquots of antibiotics

Put 0.203g of Chloramphenicol in 10ml of ethanol. Aliquots of 1ml. (Do the same for Kanamycin and Ampicillin)

by Romain

  • Petri dishes

Add 250µl of antibiotics in 250ml of LB. Pour in petri dishes.

by Arnaud

  • Range of gelose with different concentration of agar

Add in 6 bottles of 500ml 2g of LB (lysogeny broth), and in each bottle add successively 1500mg (15mg/ml), 2000mg (20mg/ml), 2500mg (25mg/ml), 3000mg (30mg/ml), 3500mg (35mg/ml) and 4000mg (40mg/ml) of agar. And just before autoclaving complete each bottle with 100ml of reverse osmosis-purified water.

Members present:

  • Instructors : Solenne.
  • Students : Arnaud, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain.

Back to the calendar

Retrieved from "http://2014.igem.org/Team:Paris_Saclay/Notebook/June/30"