Team:Paris Saclay/Notebook/July/9

From 2014.igem.org

(Difference between revisions)
(Lab work)
Line 3: Line 3:
==Lab work==
==Lab work==
====Plasmid DNA Extraction====
====Plasmid DNA Extraction====
-
'''by Romain and Sean'''
+
''by Romain and Sean''
Plasmids used: DH5αZ1 pSB1C3 RBS amilCP Ter clone 9, A and B.
Plasmids used: DH5αZ1 pSB1C3 RBS amilCP Ter clone 9, A and B.
Line 10: Line 10:
====Electrophoresis====
====Electrophoresis====
-
'''by Arnaud, Romain and Sean'''
+
''by Arnaud, Romain and Sean''
-
Plasmids used:  
+
Plasmids used:
*DH5αZ1 pSB1C3 RBS amilCP Ter clone 9, A.
*DH5αZ1 pSB1C3 RBS amilCP Ter clone 9, A.
*DH5αZ1 pSB1C3 RBS amilCP Ter clone 9, B.
*DH5αZ1 pSB1C3 RBS amilCP Ter clone 9, B.
Line 21: Line 21:
====New culture====
====New culture====
-
'''by Romain'''
+
''by Romain''
New liquid cultures of:
New liquid cultures of:

Revision as of 08:52, 7 August 2014

Contents

Wednesday 9th July

Lab work

Plasmid DNA Extraction

by Romain and Sean

Plasmids used: DH5αZ1 pSB1C3 RBS amilCP Ter clone 9, A and B.

Protocol

Electrophoresis

by Arnaud, Romain and Sean

Plasmids used:

  • DH5αZ1 pSB1C3 RBS amilCP Ter clone 9, A.
  • DH5αZ1 pSB1C3 RBS amilCP Ter clone 9, B.
  • XL1-Blue pCola-ges Kan
  • pJBEI-6409 Cm

Protocol

New culture

by Romain

New liquid cultures of:

  • FNR-RBS-amilCp-Ter DH5αZ1 P5B1C3 Cm
  • BBa B0015 dble ter pSB1C3 Cm

4x 5ml LB + 20μl Chloramphenicol + a little of bacteria. We incubate cultures at 37°C.

Reunion

Conference with the team to discuss about the project. The handled topics were:

  • Definition of Biobricks to make
  • Planning of internships
  • Definition of Biobricks to render
  • Understanding the construction of consecutive genes
  • Organisation and planning of experiences to do
  • Sponsoring
  • Wiki's progress
  • Modelling's progress


Members present:

  • Instructors and advisors: Alice, Olivier, Solenne and Sylvie.
  • Students: Arnaud, Fabio, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain and Sean.

Back to the calendar