"
Team:Paris Saclay/Notebook/July/9
From 2014.igem.org
(Difference between revisions)
(→Lab work) |
|||
| Line 3: | Line 3: | ||
==Lab work== | ==Lab work== | ||
====Plasmid DNA Extraction==== | ====Plasmid DNA Extraction==== | ||
| - | + | ''by Romain and Sean'' | |
Plasmids used: DH5αZ1 pSB1C3 RBS amilCP Ter clone 9, A and B. | Plasmids used: DH5αZ1 pSB1C3 RBS amilCP Ter clone 9, A and B. | ||
| Line 10: | Line 10: | ||
====Electrophoresis==== | ====Electrophoresis==== | ||
| - | + | ''by Arnaud, Romain and Sean'' | |
| - | Plasmids used: | + | Plasmids used: |
*DH5αZ1 pSB1C3 RBS amilCP Ter clone 9, A. | *DH5αZ1 pSB1C3 RBS amilCP Ter clone 9, A. | ||
*DH5αZ1 pSB1C3 RBS amilCP Ter clone 9, B. | *DH5αZ1 pSB1C3 RBS amilCP Ter clone 9, B. | ||
| Line 21: | Line 21: | ||
====New culture==== | ====New culture==== | ||
| - | + | ''by Romain'' | |
New liquid cultures of: | New liquid cultures of: | ||
Revision as of 08:52, 7 August 2014
Contents |
Wednesday 9th July
Lab work
Plasmid DNA Extraction
by Romain and Sean
Plasmids used: DH5αZ1 pSB1C3 RBS amilCP Ter clone 9, A and B.
Electrophoresis
by Arnaud, Romain and Sean
Plasmids used:
- DH5αZ1 pSB1C3 RBS amilCP Ter clone 9, A.
- DH5αZ1 pSB1C3 RBS amilCP Ter clone 9, B.
- XL1-Blue pCola-ges Kan
- pJBEI-6409 Cm
New culture
by Romain
New liquid cultures of:
- FNR-RBS-amilCp-Ter DH5αZ1 P5B1C3 Cm
- BBa B0015 dble ter pSB1C3 Cm
4x 5ml LB + 20μl Chloramphenicol + a little of bacteria. We incubate cultures at 37°C.
Reunion
Conference with the team to discuss about the project. The handled topics were:
- Definition of Biobricks to make
- Planning of internships
- Definition of Biobricks to render
- Understanding the construction of consecutive genes
- Organisation and planning of experiences to do
- Sponsoring
- Wiki's progress
- Modelling's progress
Members present:
- Instructors and advisors: Alice, Olivier, Solenne and Sylvie.
- Students: Arnaud, Fabio, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain and Sean.
