Team:Paris Saclay/Notebook/July/9

From 2014.igem.org

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{{Team:Paris_Saclay/notebook_header}}
=Wednesday 9th July=
=Wednesday 9th July=
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==Lab work==
==Lab work==
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====Plasmid DNA Extraction====
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====1 - Plasmid DNA Extraction:====
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'''by Romain and Sean'''
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'''Romain & Sean'''
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Plasmids used: DH5αZ1 pSB1C3 RBS amilCP Ter clone 9, A and B.
Plasmids used: DH5αZ1 pSB1C3 RBS amilCP Ter clone 9, A and B.
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[https://2014.igem.org/Team:Paris_Saclay/Protocols/Extraction_of_the_Genomic_DNA_from_Bacteria_by_using_NucleoSpin®_Tissue Protocol]
[https://2014.igem.org/Team:Paris_Saclay/Protocols/Extraction_of_the_Genomic_DNA_from_Bacteria_by_using_NucleoSpin®_Tissue Protocol]
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====2 - Electrophoresis:====
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====Electrophoresis====
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'''by Arnaud, Romain and Sean'''
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'''Arnaud & Romain & Sean'''
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Plasmids used:  
Plasmids used:  
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[https://2014.igem.org/Team:Paris_Saclay/Protocols/Gel_Electrophoresis Protocol]
[https://2014.igem.org/Team:Paris_Saclay/Protocols/Gel_Electrophoresis Protocol]
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====3 - New culture:====
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====New culture====
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'''by Romain'''
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'''Romain'''
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New liquid cultures of:
New liquid cultures of:
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Members present:
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'''Members present:'''
* Instructors and advisors: Alice, Olivier, Solenne and Sylvie.
* Instructors and advisors: Alice, Olivier, Solenne and Sylvie.
* Students: Arnaud, Fabio, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain and Sean.
* Students: Arnaud, Fabio, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain and Sean.
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{{Team:Paris_Saclay/notebook_footer}}
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[https://2014.igem.org/Team:Paris_Saclay/Notebook Back to the calendar]
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Revision as of 08:51, 7 August 2014

Contents

Wednesday 9th July

Lab work

Plasmid DNA Extraction

by Romain and Sean

Plasmids used: DH5αZ1 pSB1C3 RBS amilCP Ter clone 9, A and B.

Protocol

Electrophoresis

by Arnaud, Romain and Sean

Plasmids used:

  • DH5αZ1 pSB1C3 RBS amilCP Ter clone 9, A.
  • DH5αZ1 pSB1C3 RBS amilCP Ter clone 9, B.
  • XL1-Blue pCola-ges Kan
  • pJBEI-6409 Cm

Protocol

New culture

by Romain

New liquid cultures of:

  • FNR-RBS-amilCp-Ter DH5αZ1 P5B1C3 Cm
  • BBa B0015 dble ter pSB1C3 Cm

4x 5ml LB + 20μl Chloramphenicol + a little of bacteria. We incubate cultures at 37°C.

Reunion

Conference with the team to discuss about the project. The handled topics were:

  • Definition of Biobricks to make
  • Planning of internships
  • Definition of Biobricks to render
  • Understanding the construction of consecutive genes
  • Organisation and planning of experiences to do
  • Sponsoring
  • Wiki's progress
  • Modelling's progress


Members present:

  • Instructors and advisors: Alice, Olivier, Solenne and Sylvie.
  • Students: Arnaud, Fabio, Juliette, Maher, Marie, Mathias, Mathieu, Pierre, Romain and Sean.

Back to the calendar

Retrieved from "http://2014.igem.org/Team:Paris_Saclay/Notebook/July/9"