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Team:Paris Saclay/Notebook/July/16
From 2014.igem.org
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=Wednesday 16th July= | =Wednesday 16th July= | ||
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==Lab work== | ==Lab work== | ||
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===Transformation of competent E.coli cells=== | ===Transformation of competent E.coli cells=== | ||
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''by Fabio, Maher and Romain'' | ''by Fabio, Maher and Romain'' | ||
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'''Samples:''' | '''Samples:''' | ||
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# Concentrated | # Concentrated | ||
# Undiluted | # Undiluted | ||
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'''Results:''' | '''Results:''' | ||
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# Concentrated: 99 colonies | # Concentrated: 99 colonies | ||
# Undiluted: 5 colonies | # Undiluted: 5 colonies | ||
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'''Conclusion:''' | '''Conclusion:''' | ||
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Since the first transformations experiments with the iGEM's Biobricks, none of the diluted samples have grown. The undiluted ones have grown in some cases. We conclude that for future experiments and mainly for the project, we must use concentrated cultures. | Since the first transformations experiments with the iGEM's Biobricks, none of the diluted samples have grown. The undiluted ones have grown in some cases. We conclude that for future experiments and mainly for the project, we must use concentrated cultures. | ||
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===2 - Plasmid DNA Purification=== | ===2 - Plasmid DNA Purification=== | ||
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''by Mathieu'' | ''by Mathieu'' | ||
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==Reunion== | ==Reunion== | ||
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Exposition: The life technologies software, by a ThermoFisher Scientific's representative. (morning) | Exposition: The life technologies software, by a ThermoFisher Scientific's representative. (morning) | ||
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''Arnaud represents us'' | ''Arnaud represents us'' | ||
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Revision as of 08:47, 7 August 2014
Contents |
Wednesday 16th July
Lab work
Transformation of competent E.coli cells
by Fabio, Maher and Romain
We transformed in DH5α the following Biobrick:
- BBa_J23119
Samples:
- Concentrated
- Undiluted
- Diluted 10-1
- Control
Results:
- Concentrated: 99 colonies
- Undiluted: 5 colonies
- Diluted: 0
- Control: 0
Conclusion: Since the first transformations experiments with the iGEM's Biobricks, none of the diluted samples have grown. The undiluted ones have grown in some cases. We conclude that for future experiments and mainly for the project, we must use concentrated cultures.
2 - Plasmid DNA Purification
by Mathieu
- BBa_J23100 (x2)
- BBa_J23106 (x2)
- BBa_J23114 (x2)
from Bacterial Culture made the 15th July
Reunion
Exposition: The life technologies software, by a ThermoFisher Scientific's representative. (morning)
Reunion: Lab meeting to discuss the project. (afternoon)
Meeting with Evry's Team. (night) Arnaud represents us
