Tuesday 5th August

Lab work

A - The chassis coli Odor free

PCR verification

by Romain

Results of the striates on dishes from yesterday:

For the strain MG1655Z1:

• Nothing has grown on the kan dish, but the colony grew on LB dish. That was what we expected, the kan cassette was correctly delete.

For the strain MG1655:

• Few colonies have grown on the kan dish, and all the colonies have grown on LB dish. It's a little failure.

So now, we proceed to the PCR to verify the length of the sequences.

C - Salicylate Inducible Suppressing System

Liquid Culture

by Fabio

2 liquid cultures of BBa_K1372000 with 20ml of LB and 20µl of amp (at 3pm - 150 rpm - 37 °C), from the stock made the 4st August.

D - Lemon scent

Electrophoresis

by Sean & Pierre The strains used were made the 4th August

1. 5 µl of BBa_K762100 non diluted
2. 5 µl of BBa_K762100 diluted 10^-1
3. 5 µl of BBa_K517003

Protocol

Human Pratices

by Pierre

Ethics

I wrote the begining of a global view of the evolution of the definitions of life from Aristotle to modern times.

Bibliography

• Aristotle, On the Soul
• Epicurus, Letter to Herodotus
• Descartes, Discourse on the method
• Darwin, On the origin of species
• Bichat, Physiological researches on life and death

By Hoang Vu

Continue my bibliographical research on Citral A and B and Beta-Pinene. I have now a little more than twenty reviews about various uses and properties of citral

Members there:

• Instructors and advisors: Alice and Sylvie.
• Students: Eugene, Fabio, Hoang Vu, Leila, Juliette, Pierre, Romain, Sean and Terry.

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