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Team:Paris Saclay/Protocols/PCR clean-up
From 2014.igem.org
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# After the PCR, pool your PCR result in an eppendorf 1,5ml. | # After the PCR, pool your PCR result in an eppendorf 1,5ml. | ||
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# Add 20µl MilliQ water and centrifuge at 11000g, 1 min RT. | # Add 20µl MilliQ water and centrifuge at 11000g, 1 min RT. | ||
# Collect your DNA in an eppendorf 1,5ml. | # Collect your DNA in an eppendorf 1,5ml. | ||
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Revision as of 12:58, 5 August 2014
PCR clean-up
- After the PCR, pool your PCR result in an eppendorf 1,5ml.
- Add 200µl NTI.
- Centrifuge at 11000g, 30 secondes RT.
- Discard the supernatant, wash first time with 700µl NT3.
- Centrifuge at 11000g, 30 secondes RT.
- Discard the supernatant, wash a second time with 700µl NT3.
- Centrifuge at 11000g, 30 secondes RT.
- Centrifuge the column empty.
- Add 20µl MilliQ water and centrifuge at 11000g, 1 min RT.
- Collect your DNA in an eppendorf 1,5ml.
