Team:Exeter/invivo

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<div id="toctitle"><h2>Contents</h2></div>
 
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<li class="toclevel-1"><a href="#1"><span class="tocnumber">1.</span> <span class="toctext">Introduction</span></a></li>
 
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<li class="toclevel-2"><a href="#1.1"><span class="tocnumber">1.1</span> <span class="toctext">Aim</span></a></li>
 
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<li class="toclevel-2"><a href="#1.2"><span class="tocnumber">1.2</span> <span class="toctext">Raman Spectroscopy</span></a></li>
 
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<li class="toclevel-2"><a href="#1.3"><span class="tocnumber">1.3</span> <span class="toctext">The Partition Coefficient</span></a></li>
 
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<li class="toclevel-1"><a href="#2"><span class="tocnumber">2.</span> <span class="toctext">Experimental Method</span></a></li>
 
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<li class="toclevel-2"><a href="#2.1"><span class="tocnumber">2.1</span> <span class="toctext">The Standard Curve</span></a></li>
 
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<li class="toclevel-2"><a href="#2.2"><span class="tocnumber">2.2</span> <span class="toctext">Measuring degradation of TNT and NG <i>in vivo</i></span></a></li>
 
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<li class="toclevel-2"><a href="#2.3"><span class="tocnumber">2.3</span> <span class="toctext">The Partition Coefficient</span></a></li>
 
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<li class="toclevel-1"><a href="#3"><span class="tocnumber">3.</span> <span class="toctext">Results, Analysis and Discussion</span></a></li>
 
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<li class="toclevel-2"><a href="#3.1"><span class="tocnumber">3.1</span> <span class="toctext">The Raman spectrum of TNT and NG </span></a></li>
 
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<li class="toclevel-2"><a href="#3.3"><span class="tocnumber">3.3</span> <span class="toctext">Measuring degradation of NG <i>in vivo</i></span></a></li>
 
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<li class="toclevel-2"><a href="#3.4"><span class="tocnumber">3.4</span> <span class="toctext">The Partition Coefficient</span></a></li>
 
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<li class="toclevel-1"><a href="#4"><span class="tocnumber">4.</span> <span class="toctext">Summary</span></a></li>
 
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<li class="toclevel-1"><a href="#5"><span class="tocnumber">5.</span> <span class="toctext">Referances</span></a></li>
 
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<h1> <span id="0"> Measuring <i>in vivo</i> degredation using Raman spectroscopy </span> </h1>
<h1> <span id="0"> Measuring <i>in vivo</i> degredation using Raman spectroscopy </span> </h1>

Revision as of 21:02, 17 October 2014

Exeter | ERASE

Measuring in vivo degredation using Raman spectroscopy

Introduction

Aim

The aim of this experiment was to measure the degradation rate of trinitrotoluene (TNT) and nitroglycerin (NG) in vivo using Raman spectroscopy. The degradation rate of enzymes Nem-A and Xen-B when transcribed by an E. coli bacterium will be measured. In addition to the degradation rates, a value for the partition coefficient for TNT and NG between water and the cell membrane was determined.

TNT Degradation

During the NemA and XenB-catalysed degradation of TNT, a series of nitrite groups as well aromatic ring reduction leads to formation of amino-dimethyl-tetranitrobiphenyl. During this process a hydride-Meisenheimer complex metabolite is formed. This metabolite has a distinct dark-brown colour [Vorbeck et.al 1994]. This metabolite causes reaction mixtures with XenB or NemA, mixed with TNT, to change from colourless to red, then to yellow [Pak 2000].

References

  • Vorbeck, Claudia; Lenke, Hiltrud; Fischer, Peter; Hans-Joachim, Knackmuss (1994) Identification of a Hydride-MeisenheimerComplex as a Metabolite of 2,4,6-Trinitrotoluene by a Mycobacterium Strain ; Journal of Bacteriology
  • Jeong W. Pak; Kyle L. Knoke; Daniel R. Noguera; Brian G. Fox; Glenn H. Chambliss (2000) Transformation of 2,4,6-Trinitrotoluene by Purified Xenobiotic Reductase B from Pseudomonas fluorescens I-C; Applied and Environmental Microbiology

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