Tuesday 5th August

Lab work

A - The chassis coli Odor free

PCR verification

by Romain

Results of the striates on dishes from yesterday:

For the strain MG1655Z1:

• Nothing has grown on the kan dish, but the colony grew on LB dish. That was what we expected, the kan cassette was correctly delete.

For the strain MG1655:

• Few colonies have grown on the kan dish, and all the colonies have grown on LB dish. It's a little failure.

So now, we proceed to the PCR to verify the length of the sequences.

Strains used: E. coli MG1655Z1 and E. coli MG1655, and oligonucleotides used: iPS75 bis and iPS76 bis.

Protocol

Add into a PCR tube the following:

We follow these quantities for 11 tubes: 5 tubes for the strain MG1655, 5 tubes for the strain MG1655Z1 and 1 tube with bacteria which had grown on the kan dish. Tube was placed in PCR machine with the following parameters.

The different bacterial cultures in each tubes:

• Tube 1 to 5: MG1655
• Tube 6 to 10: MG1655Z1
• Tube 11: MG1655 which had grown on the kan dish.

The electrophoresis results:

• 1. 10 µl of ladder
• 2 to 6. 10 µl in each tube of PCR for the strain MG1655
• 7 to 11. 10 µl in each tube of PCR for the strain MG1655Z1
• 12. 10µl of PCR for the MG1655 which had grown on the kan dish

Results of the PCR: The electrophoresis revealed correctly the expected size of the sequence, except for the holes 2 and 11. The kan resistance sequence had been successfully removed.

Liquid cultures overnight made: 3ml LB medium + 500µl transformed strain MG1655 + Cm

C - Salicylate Inducible Suppressing System

Liquid Culture

by Fabio

2 liquid cultures of BBa_K1372000 with 20ml of LB and 20µl of amp (at 3pm - 150 rpm - 37 °C), from the stock made the 4th August.

D - Lemon scent

Electrophoresis

by Sean & Pierre The strains used were made the 4th August

1. 5 µl of BBa_K762100 non diluted
2. 5 µl of BBa_K762100 diluted 10^-1
3. 5 µl of BBa_K517003

Protocol

Human Practices

by Pierre

Ethics

I wrote the begining of a global view of the evolution of the definitions of life from Aristotle to modern times.

Bibliography

• Aristotle, On the Soul
• Epicurus, Letter to Herodotus
• Descartes, Discourse on the method
• Darwin, On the origin of species
• Bichat, Physiological researches on life and death

by Hoang Vu

Continue my bibliographical research on Citral A and B and Beta-Pinene. I have now a little more than twenty reviews about various uses and properties of citral

Art & Design

by Leila & Juliette

We tested some different concentration of agar in LAG, how we can hang it, how it looks and how easy it is to turn it out : 15 g/l, 20 g/l, 25 g/l, 30 g/l, 35 g/l.

Finally, we though our test were effective and we decided to select only 3 concentration for the future : 20 g/l, 25 g/l and 30 g/l.

Members there:

• Instructors and advisors: Alice and Sylvie.
• Students: Eugene, Fabio, Hoang Vu, Leila, Juliette, Pierre, Romain, Sean and Terry.

Photo of the Day

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