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Team:Paris Saclay/Notebook/September/1
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We need to work on this plasmid tomorrow so we do some culture from the stock | We need to work on this plasmid tomorrow so we do some culture from the stock | ||
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Revision as of 18:22, 29 September 2014
Contents |
Lab Work
B-Construction of the fusion protein
'by Hoang Vu'
We picked out white colonies from Xgal-IPTG petri dish and blue colonies found in non Xgal-IPTG petri dish in a petri dish that contain Ampicillin to check if the blue ones are not came from a carrying of Xgal-IPTG during the precedent pick out.
D-Lemon scent
by melanie
Limone synthase
We had a problem to clone LS in pGMETeasy and pPSI. So from the dish of streaking from 26 AugustWe do PCR of all the clones:
We use the Protocol - PCR for bacterial culture and we use the same PCR condition than on the August 8 but with the appropriate Primer and Biobrick.
PPS5
We check if we have the right insert in our plasmid pPS5 : CAD (cinnalyl alcool deshydrogenase) So we do a PCR in the same condition than August 20
PPS3 and PPS4
We need to work on this plasmid tomorrow so we do some culture from the stock
