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Team:Paris Saclay/Notebook/August/27
From 2014.igem.org
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Three colonies were selected from the gel prepared earlier by Laetitia, namely #3,#4, and #6. The three gel samples underwent PCR clean-up. | Three colonies were selected from the gel prepared earlier by Laetitia, namely #3,#4, and #6. The three gel samples underwent PCR clean-up. | ||
| - | ====Gel electrophoresis of | + | ====Gel electrophoresis of PCR clean-up results containing pGEMTeasy+chromoprotein==== |
''by Sean'' | ''by Sean'' | ||
| - | + | PCR clean-up was performed ealier [https://2014.igem.org/Team:Paris_Saclay/Notebook/August/27#PCR_clean-up_of_colonies_containing_pGEMTeasy.2Bchromoprotein today]. 1µl of each result was used for the electrophoresis. Nothing appeared under UV. | |
===D - lemon scent === | ===D - lemon scent === | ||
Revision as of 16:53, 27 August 2014
Contents |
Wednesday 27th August
Lab Work
B - Construction of the fusion protein (color)
PCR clean-up of colonies containing pGEMTeasy+chromoprotein
by Sean
Three colonies were selected from the gel prepared earlier by Laetitia, namely #3,#4, and #6. The three gel samples underwent PCR clean-up.
Gel electrophoresis of PCR clean-up results containing pGEMTeasy+chromoprotein
by Sean
PCR clean-up was performed ealier today. 1µl of each result was used for the electrophoresis. Nothing appeared under UV.
D - lemon scent
Plasmide extraction
By Mélanie
Extraction of pPS5 with the phenol protocol (as previously described)
PCR of Limonene synthase (BBa762100)
by Mélanie
5 tubes to do a gradient
| components | volumes |
|---|---|
| H2O | 29.75μl |
| Gotaq buffer | 10µl |
| dNTP 10mM | 1µl |
| iPS67 | 1µM |
| iPS66 | 1µM |
| DNA | 1µl |
| MgCl2 | 4µl |
| Gotaq | 1.25µl |
| step | temperature (°C) | time |
|---|---|---|
| 1 | 95 | 2min |
| 2 | 95 | 30 sec |
| 3 | 49-55 (gradient) | 30sec |
| 4 | 72 | 2min |
| 5 | 72 | 5min |
